Molecular and serotyping characterization of shiga toxogenic Escherichia coli associated with food collected from Saudi Arabia

Al-Zogibi, Onizan G.; Mohamed, Moussa I.; Hessain, Ashgan M.; El-Jakee, J.; Kabli, Saleh A.

doi:10.1016/j.sjbs.2015.02.019

Cited by 19 publications

(23 citation statements)

References 29 publications

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“…Almost one third (28.9%) of the examined bovine milk in this study showed the existence of either or both E. coli or S. aureus pathogens. E. coli was detected in 13.2% of the examined milk samples, which was comparable with previous report (15.9%) in Saudi Arabia [24]. Higher prevalence was recorded in Ethiopia (29.6%) by Garedew et al [4].…”

Section: Discussionsupporting

confidence: 87%

Public health risks of Escherichia coli and Staphylococcus aureus in raw bovine milk sold in informal markets in Egypt

Elmonir

Abo-Remela

Sobeih

2018

J Infect Dev Ctries

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Introduction: Milk is an important food in Egypt and most of it is sold as raw milk in informal markets. Methodology: This study was conducted to investigate the public health risks of Escherichia coli and Staphylococcus aureus in milk sold in informal markets in Egypt. A total of 121 milk samples were analyzed for occurrence, virulence genes and antibiotic resistance of E. coli and S. aureus. Results: A total of 35/121 (28.9%) of milk samples were contaminated with 16/121 (13.2%) E. coli, 22/121 (18.2%) S. aureus, and 3/121 (2.5%) both isolates. Shiga-toxin producing E. coli (STEC), Enterotoxigenic E. coli (ETEC) and Enterotoxigenic S. aureus were detected in 5/121 (4.1%), 2/121 (1.7%) and 8/121 (6.6%) of the examined milk samples, respectively. Multiple drug resistances (MDRs) were showed by 14/16 (87.5%) and 21/22 (95.5%) of E. coli and S. aureus isolates, respectively. E. coli isolates showed high resistance for cephalothin (87.5%), ampicillin (68.8%) and tetracycline (68.8%), but were sensitive for gentamicin and chloramphenicol. Resistance phenotypes of E. coli were diverse; however, STEC isolates were significantly associated with co-resistance to cephalothin, ampicillin and tetracycline (P< 0.05). Two (9.1%) of S. aureus isolates were methicillin-resistant (MRSA) but sensitive to gentamicin (GS-MRSA). Five (22.7%) of S. aureus isolates were gentamicin-resistant methicillin-sensitive S. aureus (GR-MSSA). S. aureus isolates also showed high resistance for ampicillin (100%), tetracycline (90.1%) and sulfamethoxazole-trimethoprim (90.1%). Conclusion: These findings highlighted the potential public health hazards of E. coli and S. aureus pathogens in raw milk sold in informal markets in Egypt.

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Section: Discussionsupporting

confidence: 87%

Public health risks of Escherichia coli and Staphylococcus aureus in raw bovine milk sold in informal markets in Egypt

Elmonir

Abo-Remela

Sobeih

2018

J Infect Dev Ctries

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“…Furthermore, several studies have demonstrated the activity of essential oils against pathogens in food systems [7][8][9]. In the same way, the present study shows that under the effect of M. suaveolens essential oil; there is an important abatement of S. aureus and E. coli during the storage period, these pathogens have been cited among the most incriminated in food poisoning [43][44][45][46]. In addition, the use of essential oils in food preservation makes it possible to improve the taste quality and to give a taste appreciated for the consumer [7].…”

Section: Discussionsupporting

confidence: 78%

Application of Mentha suaveolens essential oil as an antimicrobial agent in fresh turkey sausages

2018

J App Biol Biotech

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The aim of this study is to evaluate the antimicrobial effect of Mentha suaveolens essential oil against pathogenic bacteria in fresh turkey sausages. The essential oil was extracted by hydrodistillation. The antibacterial activity was carried out by agar diffusion and microplates methods against Escherichia coli, Salmonella, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumonia, and Streptococcus faecalis. The antioxidant activity was carried out by ferric reducing antioxidant power and free radical scavenging activities against 2,2-diphenyl-1-picrylhydrazyl. The antimicrobial effect on sausages was conducted by the enumeration of S. aureus and E. coli during the storage period of fresh sausages manufactured with different concentrations of essential oil. The results showed that the essential oil of M. suaveolens has an antibacterial effect against Gram-negative and Gram-positive bacteria in addition to its antioxidant activity (EC 50 = 3.95 ± 0.03 mg/mL and IC 50 = 3.11 ± 0.02 mg/mL). Moreover, the addition of essential oil to fresh sausages has a significant effect against the tested pathogenic bacteria. The present data clearly demonstrate that the essential oil of M. suaveolens has a remarkable antimicrobial and antioxidant activities and can be used as a food additive to extend the shelf life of food products.

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“…In Egypt, cattle, goats and sheep are considered a potential source of E. coli infection to humans, with the important serotypes are O26, O86, O111, O126, O127, O157, O158 and O164 [34]. They shed the bacteria into the environment throughout their faeces without even suffering from clinical disease, thus allowing meat and milk contamination causing human foodborne illness following consumption of such food [35]. In this regard, Food Regulations Act stated that coliform count shouldn't exceed 1.7 log CFU/mL and E. coli shouldn't be present in even one mL of a milk sample [29].…”

Section: Discussionmentioning

confidence: 99%

Zoonotic Importance of Some Pathogenic Bacteria Isolated from Small Ruminants' Milk and Hands of Dairy Workers

El-Mahallawy

Elhariri

Elhelw

et al. 2017

Zagazig Veterinary Journal

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Staphylococcus aureus and enteropathogenic Escherichia coli are among zoonotic bacterial food-borne pathogens causing illness ranged from diarrhoea to fatal conditions. This study was undertaken to evaluate the occurrence of Staph. aureus and E. coli in small ruminants' raw milk and hands of dairy workers at small-scale production units, Giza, Egypt. A total of 420 raw milk samples were obtained from apparently healthy sheep and goats, also, hand swabs (n=46) from workers at the units under investigation were examined. Overall, Staph. aureus (13.1%, 55/420) and E. coli (26.2%, 110/420) isolates were obtained from raw milk of sheep and goats. However, only Staph. aureus (15.2%, 7/46) was recovered from hand swabs of dairy workers. Using PCR, all the tested Staph. aureus isolates from milk and hand swabs yielded specific bands of Thermonuclease (nuc) gene at 279 bp, however, amplification of virulence (eae) gene encoding the intimin protein of E. coli produced an amplicon of 917 bp in 45% of the tested E. coli isolates. In conclusion, our findings provide an overview about Staph. aureus and E. coli contamination in raw milk of small ruminants locally bred by smallholders and suggest probably transmission of Staph. aureus from hands of dairy workers that contaminate the milk or udder of apparently healthy animals. Reporting of such organisms from milk indicates the need for strict hygienic measures during milking at these production units.

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Molecular and serotyping characterization of shiga toxogenic Escherichia coli associated with food collected from Saudi Arabia

Cited by 19 publications

References 29 publications

Public health risks of Escherichia coli and Staphylococcus aureus in raw bovine milk sold in informal markets in Egypt

Public health risks of Escherichia coli and Staphylococcus aureus in raw bovine milk sold in informal markets in Egypt

Application of Mentha suaveolens essential oil as an antimicrobial agent in fresh turkey sausages

Zoonotic Importance of Some Pathogenic Bacteria Isolated from Small Ruminants' Milk and Hands of Dairy Workers

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