Molecular and Serological Survey of Selected Viruses in Free-Ranging Wild Ruminants in Iran

Hemmatzadeh, Farhid; Boardman, Wayne; Alinejad, A; Hematzade, Azar; Moghadam, Majid Kharazian

doi:10.1371/journal.pone.0168756

Cited by 20 publications

(16 citation statements)

References 69 publications

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“…Presence of wildlife population along the national park in Borgu, Niger State, Nigeria, where cloven-hoofed species come in contact with livestock was shown to be the probable exposure factor that contributed to high FMD seropositivity in livestock observed in the area [37]. e results from this study corroborate with other studies in South Africa, Zimbabwe, Zambia, Botswana, Namibia, India, Chad, and Iran that demonstrated FMDV antibodies in wildlife [10,11,28,[40][41][42][43][44][45]. High FMDV prevalence in waterbucks observed in this study reflects their ecology and living ecosystem which is consistent with other findings in East Africa and Zimbabwe [27,41,46].…”

Section: Discussionsupporting

confidence: 86%

Seroprevalence of Foot and Mouth Disease Virus Infection in Some Wildlife and Cattle in Bauchi State, Nigeria

Atuman

Kudi

Abdu

et al. 2020

Veterinary Medicine International

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Foot and mouth disease (FMD) is an important transboundary viral disease of both domestic and wild cloven-hoofed animals characterized by high morbidity with devastating consequence on the livestock worldwide. Despite the endemic nature of FMD in Nigeria, little is known about the epidemiology of the disease at the wildlife-livestock interface level. To address this gap, blood samples were collected between 2013 and 2015 from some wildlife and cattle, respectively, within and around the Yankari Game Reserve and Sumu Wildlife Park in Bauchi State, Nigeria. Wild animals were immobilized using a combination of etorphine hydrochloride (M99 ® Krüger-Med South Africa) at 0.5-2 mg/kg and azaperone (Stresnil ® , Janssen Pharmaceuticals (Pty.) Ltd., South Africa) at 0.1 mg/kg using a Dan-Inject ® rifle (Dan-Inject APS, Sellerup Skovvej, Denmark) fitted with a 3 ml dart syringe and for reversal, naltrexone (Trexonil ® Kruger-Med South Africa) at 1.5 mg IM was used, and cattle were restrained by the owners for blood collection. Harvested sera from blood were screened for presence of antibodies against the foot and mouth disease virus (FMDV) using the PrioCHECK ® 3ABC NSP ELISA kit, and positive samples were serotyped using solid-phase competitive ELISA, (IZSLER Brescia, Italy). Out of the 353 sera collected from cattle and wildlife 197 (65.7%) and 13 (24.5%) (P < 0.05), respectively, tested positive for antibodies to the highly conserved nonstructural 3ABC protein of FMDV by the FMDV-NS blocking ELISA. Classification of cattle into breed and sex showed that detectable antibodies to FMDV were higher (P < 0.05) in White Fulani 157 (72.8%) than in Red Bororo 23 (39.7%) and Sokoto Gudali 17 (33.3%) breeds of cattle, whereas in females, detectable FMDV antibodies were higher (P < 0.05) 150 (72.8%) than in males 47 (50.0%). In the wildlife species, antibodies to FMDV were detected in the waterbucks 2 (28.6%), elephant 1 (25.0%), wildebeests 4 (33.3%), and elands 6 (25.0%). Four serotypes of FMDV: O, A, SAT 1, and SAT 2 were detected from the 3ABC positive reactors in waterbucks, elephants, wildebeests, and elands. e results showed presence of antibodies to FMDV in some wildlife and cattle and suggested that wildlife could equally play an important role in the overall epidemiology of FMD in Nigeria. FMD surveillance system, control, and prevention program should be intensified in the study area.

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Section: Discussionsupporting

confidence: 86%

Seroprevalence of Foot and Mouth Disease Virus Infection in Some Wildlife and Cattle in Bauchi State, Nigeria

Atuman

Kudi

Abdu

et al. 2020

Veterinary Medicine International

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“…MCF cases have been documented in both India (15) and Iran (16,17), which border Pakistan. In Pakistan in most rural areas, livestock farms, animal hospitals and research centres, it is a common practice to keep MCF reservoir and susceptible animals in close proximity (18,19).…”

Section: Introductionmentioning

confidence: 99%

WITHDRAWN: First Study of Molecular detection of Ovine Herpesvirus 2 DNA using heminested PCR in Pakistan

Riaz

Dry

Dalziel

et al. 2020

Preprint

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Background Malignant catarrhal fever (MCF) is a highly fatal lymphoproliferative disease of cattle, deer, bison, water buffalo and pigs caused by the gamma herpesviruses; alcelaphine herpesvirus 1 (AlHV-1) and ovine herpesvirus 2 (OvHV-2). AlHV-1 is known to cause wildebeest-associated MCF whereas OvHV-2 is known to cause sheep-associated MCF. AlHV-1 and OvHV-2 cause inapparent infection in their reservoir hosts (wildebeest for AlHV-1 and sheep for OvHV-2) but fatal lymphoproliferative disease in susceptible species. Methods This study was aimed to find prevalence of OvHV-2 in sheep, goat, cattle and buffalo in Rawalpindi and Islamabad cities of Pakistan, using molecular and phylogenetic methods. Blood samples were collected consisting of sheep (n = 54), goat (n = 50), cattle (n = 46) and buffaloes (n = 50) from slaughter houses and farms. The samples were subjected to hemi-nested PCR, sequencing, and phylogenetic analysis of OvHV-2 POL gene and OvHV-2 tegument protein gene. Results The highest percentage of positive samples was found to be in sheep (13%) whereas positive samples from goat, cattle and buffaloes had percentage of 11%, 9% and 6.5% respectively. Four positive PCR products from sheep samples were also sequenced. The sequences obtained were submitted to NCBI GenBank database under the accession numbers of MK852173 for POL gene, and MK840962, MK852171 and MK852172 for ORF75 tegument protein gene. Phylogenetic analysis revealed close identities with some sequences of this virus from worldwide including neighbouring countries including China, India and Iran. Conclusion This study is the first study in Pakistan on the prevalence of OvHV-2 in carrier and susceptible species. This report provides important data about the presence of OvHV-2 in apparently healthy cattle and buffaloes, which might transmitted to them from OvHV-2 positive sheep or goats. It is concluded that a countrywide, large scale OvHV-2 prevalence study should be conducted to avoid economic losses due to any outbreak of MCF.

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“…This finding being the first of its kind in the study area reveals that FMD could be a problem in wildlife in Nigeria. This is not surprising as FMD is endemic in Nigerian livestock (18, 20, 39, 23). Presence of wildlife population along the national park in Borgu Niger State Nigeria where cloven hoofed species come in contact with live stock was shown to be the probable exposure factor that contributed to high FMD sero-positivity in livestock observed in the area (37).…”

Section: Discussionmentioning

confidence: 95%

“…The first reported case of FMD outbreak in Nigeria was in 1924, which was attributed to type O virus (16). Subsequently, other serotypes (A, SAT 1 and SAT 2) were reported (17, 18, 19, 20, 21, 22) and recently SAT 3 serotype (23).…”

Section: Introductionmentioning

confidence: 99%

Seroprevalence of Foot and Mouth Disease Virus Infection in some Wildlife and Cattle in Bauchi State, Nigeria

Atuman

Kudi

Abdu

et al. 2019

Preprint

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18Background 19 Foot and mouth disease (FMD) is one of the most economically important transboundary 20 animal diseases with devastating consequence on livestock production and wildlife 21 conservation. The objectives of the study were: to determine the seroprevalence of FMDV in 22 wildlife and cattle and identify circulating FMDV serotypes in wildlife and identify potential 23 risk factors that will contribute to transmission of the disease at the wildlife-livestock 24 interface in Yankari Game Reserve and Sumu Wildlife Park in Bauchi State, Nigeria. 25 Methods 26Blood samples were collected between 2013 to 2015 from some wildlife and cattle 27 respectively within and around Yankari Game Reserve (YGR) and Sumu Wildlife Park 28 (SWP) in Bauchi State, Nigeria. The Wild animals were immobilized for blood collection 29 using a combination of Etorphine Hydrochloride (M99® Krüger-Med South Africa ) at 0.5-2 30 mg/kg and Azaperone (Stresnil®, Janssen Pharmaceuticals (Pty.) Ltd., South Africa) at 0.1 31 mg/kg using a Dan-Inject® rifle (Dan-Inject APS, Sellerup Skovvej, Denmark) fitted with 3 32 ml dart syringe and for reversal, Naltrexone (Trexonil® Kruger-Med South Africa) at 1.5 mg 33 IM was used, cattle were restrained by the owners for blood collection. Harvested Sera from 34 blood were screened for presence of Antibodies against FMDV using prioCHECK® 3 ABC 35 NSP ELISA kit and positive samples from wildlife were serotyped using Solid-Phase 36Competitive ELISA, (IZSLER Brescia-Italy). Data obtained were analysed using Graphpad 37 Prism version 7. 38 Results 39The results showed that 197 (65.7%) of the 300 serum samples from cattle and 13 (24.5%) of 40 the 53 serum samples from wildlife tested positive for antibodies to the highly conserved 2 41 non-structural 3-ABC protein of FMDV and statistically significant (P <0.05). Classification 42 of cattle into breed and sex showed that detectable antibodies to FMDV were higher (P 43 <0.05) in White Fulani 157 (72.8%) than red Bororo 23 (39.7%) and Sokoto Gudali 17 44 (33.3%) breeds of cattle whereas in females detectable FMDV antibodies were higher (P 45 <0.05) 150 (72.8%) than in males 47 (50.0%). In the wildlife species, antibodies to FMDV 46 were detected in waterbuck 2 (28.6%), elephant 1 (25.0%), wildebeest 4 (33.3%) and eland 6 47 (25.0%). Four serotypes of FMDV: O, A, SAT-1 and SAT-2 were detected from the 3-ABC 48 positive reactors in waterbuck, elephant, wildebeest and eland. Contact of wildlife and cattle 49 during utilization of the rich resources in the conservation areas is a potential risk factor for 50 the spread of FMDV in the study area. 51 Conclusions 52 Presence of FMDV antibodies in cattle and some wildlife were observed and serotypes of 53 FMDV: O, A, SAT-1 and SAT-2 were detected from the 3-ABC positive reactors in some of 54 the wildlife. The study highlights the need for active surveillance of FMDV in wildlife and 55 pastoral cattle within and around wildlife conservation areas in Nigeria. FMD surveillance 56 system, control and prevention progra...

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Molecular and Serological Survey of Selected Viruses in Free-Ranging Wild Ruminants in Iran

Cited by 20 publications

References 69 publications

Seroprevalence of Foot and Mouth Disease Virus Infection in Some Wildlife and Cattle in Bauchi State, Nigeria

Seroprevalence of Foot and Mouth Disease Virus Infection in Some Wildlife and Cattle in Bauchi State, Nigeria

WITHDRAWN: First Study of Molecular detection of Ovine Herpesvirus 2 DNA using heminested PCR in Pakistan

Seroprevalence of Foot and Mouth Disease Virus Infection in some Wildlife and Cattle in Bauchi State, Nigeria

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