Molecular and serological surveillance of Hepatitis E virus in wild and domestic carnivores in Brandenburg, Germany

Dähnert, Lisa; Conraths, Franz Josef; Reimer, Nicole; Groschup, Martin H.; Eiden, Martin

doi:10.1111/tbed.12877

Cited by 28 publications

(33 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These food items have been suggested to be a source of HEV (10,12,39) and are frequently imported to Northern Norway. Recently, an anti-HEV IgG seroprevalence of 56.6% and 32.2% was found in dogs and cats in Germany (40). Since no viral RNA could be isolated, the genotype is still not known.…”

Section: Discussionmentioning

confidence: 99%

Seroprevalence of hepatitis E virus (HEV) in a general adult population in Northern Norway: the Tromsø study

Olsøy

Henriksen

Weissbach

et al. 2019

Med Microbiol Immunol

View full text Add to dashboard Cite

Hepatitis E virus (HEV) is a major cause of acute viral hepatitis in many parts of the world but only a few cases have been diagnosed in Norway. To investigate the HEV exposure rate in a presumed low-risk area, we have conducted a population-based study of anti-HEV IgG seroprevalence in Northern Norway. A total of 1800 serum samples from 900 women and 900 men, age 40-79 years, were randomly selected from the 21083 participants in the 7 th Tromsø Study, representing the 32591 inhabitants of the Tromsø municipality that were ≥40 years. All samples were analyzed by ELISA-1 (recomWell HEV IgG). Samples testing positive or borderline, as well as a 1.5-fold excess of negative samples, were retested by ELISA-2 (DiaPro HEV IgG). If still borderline or a result discordant from ELISA-1, the sample was retested by ELISA-3 (Wantai HEV IgG) and strip-immunoassay (recomLine HEV IgG). Anti-HEV IgG was detected in 205 individuals (11.4%), yielding an estimated seroprevalence of 10.4% in the age-matched population of Tromsø. Using logistic regression analysis followed by multivariable backward elimination analysis, increasing age (OR 1.036; p<0.001) and higher education (OR 2.167; p<0.001) were found as potential risk factors, whereas travel abroad or eating of red meat was not. Our results indicate that HEV-infection is common in Northern Norway and suggest that HEV testing should be included in the evaluation of elevated liver enzymes.

show abstract

Section: Discussionmentioning

confidence: 99%

Seroprevalence of hepatitis E virus (HEV) in a general adult population in Northern Norway: the Tromsø study

Olsøy

Henriksen

Weissbach

et al. 2019

Med Microbiol Immunol

View full text Add to dashboard Cite

show abstract

“…To investigate the prevalence of HEV in domestic pets in the Netherlands, we have collected serum samples from 162 dogs, we found that 18.52% (30/162) dogs were positive for anti-HEV antibodies in serum. Literature data on the seroprevalence of HEV antibodies in dogs ranges from 0.8% in UK [11], 28.2% in South Korea [7], 13.54 to 36.55% in different regions of China [6,[12][13][14], and 56.6% in Germany [15]. Genomic HEV RNA was not detectable in these serum samples by qRT-PCR.…”

Section: Resultsmentioning

confidence: 83%

Hepatitis E virus seroprevalence in pets in the Netherlands and the permissiveness of canine liver cells to the infection

Spee

et al. 2020

Ir Vet J

View full text Add to dashboard Cite

Hepatitis E virus (HEV) as an emerging zoonotic pathogen causes a major public health issue. Transmission from domestic, wildlife and zoo animals to human has been widely reported. Whether pets also serve as reservoirs remains an intriguing question. In this study, we found the sero-positive rates of HEV-specific antibodies in pet dogs, cats and horses of 18.52% (30/162), 14.89% (7/47) and 18.18% (4/22) in the Netherlands. Although HEV viral RNA was not detected in these animals, we have demonstrated that dog liver cells are susceptible to HEV infection in vitro. These results call more attention to address the potential role of pets in the zoonotic transmission of HEV.

show abstract

“…Although other alternatives to serum have been tested for the detection of anti-HEV antibodies, they are scarce, and the results have not always been compared with the serum results. A previous study detected anti-HEV antibodies in body cavity transudate obtained from raccoons, raccoon dogs, cats, and dogs [47]. Meat juice has also been used in some studies to determine HEV seroprevalence in hunted wild boars and slaughtered pigs with satisfactory results [48,56].…”

Section: Discussionmentioning

confidence: 99%

“…The enzyme-linked immunosorbent assay (ELISA) technique is the main diagnostic method to perform HEV surveillance studies, and many commercial and 'in-house' kits have been described [44][45][46]. Serum samples are typically used to assay anti-HEV antibodies, although meat juice and body cavity transudate have also been used in a few studies [47,48]. To detect the presence of HEV RNA, several reverse transcriptase-polymerase chain reactions (RT-PCR) techniques have been described, with the method proposed by Jothikumar et al in 2006, one of the most frequently used because of its high specificity and sensitivity [49,50].…”

Section: Introductionmentioning

confidence: 99%

Liver Transudate, a Potential Alternative to Detect Anti-Hepatitis E Virus Antibodies in Pigs and Wild Boars (Sus scrofa)

et al. 2020

View full text Add to dashboard Cite

In recent years, cases of hepatitis E virus (HEV) infection have increased in Europe in association with the consumption of contaminated food, mainly from pork products but also from wild boars. The animal’s serum is usually tested for the presence of anti-HEV antibodies and viral RNA but, in many cases such as during hunting, an adequate serum sample cannot be obtained. In the present study, liver transudate was evaluated as an alternative matrix to serum for HEV detection. A total of 125 sera and liver transudates were tested by enzyme-linked immunosorbent assay at different dilutions (1:2, 1:10, 1:20), while 58 samples of serum and liver transudate were checked for the presence of HEV RNA by RT-qPCR. Anti- HEV antibodies were detected by ELISA in 68.0% of the serum samples, and in 61.6% of the undiluted transudate, and in 70.4%, 56.8%, and 44.8% of 1:2, 1:10, or 1:20 diluted transudate, respectively. The best results were obtained for the liver transudate at 1:10 dilution, based on the Kappa statistic (0.630) and intraclass correlation coefficient (0.841). HEV RNA was detected by RT-qPCR in 22.4% of the serum samples and 6.9% of the transudate samples, all samples used for RT-qPCR were positive by ELISA. Our results indicate that liver transudate may be an alternative matrix to serum for the detection of anti-HEV antibodies.

show abstract

Molecular and serological surveillance of Hepatitis E virus in wild and domestic carnivores in Brandenburg, Germany

Cited by 28 publications

References 11 publications

Seroprevalence of hepatitis E virus (HEV) in a general adult population in Northern Norway: the Tromsø study

Seroprevalence of hepatitis E virus (HEV) in a general adult population in Northern Norway: the Tromsø study

Hepatitis E virus seroprevalence in pets in the Netherlands and the permissiveness of canine liver cells to the infection

Liver Transudate, a Potential Alternative to Detect Anti-Hepatitis E Virus Antibodies in Pigs and Wild Boars (Sus scrofa)

Contact Info

Product

Resources

About