Molecular and serological diagnosis of Chikungunya virus infection

Chikungunya Virus Infection during Pregnancy, Réunion, France, 2006

“…We excluded women who had positive serologic results but did not report symptoms or have a positive RT-PCR result because we could not identify the date of infection. Studies during the outbreak in Réunion showed that IgM tended to persist for 12 to 24 months and cannot be used to identify the date of infection ( 21 ). …”

Expression of the capsid protein of Chikungunya virus in a baculovirus for serodiagnosis of Chikungunya disease

“…Serological diagnostic methods such as the hemagglutination inhibition test (HI test) and ELISA have also been used to diagnose CHIKV disease. Although the HI test is a simple and rapid test, the results can be difficult to interpret due to cross-reactivity with other viruses (Chhabra et al, 2008;Grivard et al, 2007;Karabatsos, 1975). ELISA is another popular method to detect viral antigen-specific antibodies due to its high sensitivity and specificity, and ELISA makes it possible to screen a large number of small volume samples (Kitagawa et al, 1983;Thein et al, 1992).…”

“…Currently, serological tests for the diagnosis of CHIK fever are the hemagglutination test (HI test), enzyme-linked immunosorbent assays (ELISA), and indirect immunofluorescence test (Grivard et al, 2007;Karabatsos, 1975;Litzba et al, 2008). Presently, whole virus antigens have been used as a diagnostic reagent for the diagnosis of CHIK fever.…”

Development of Field-Based Real-Time Reverse Transcription–Polymerase Chain Reaction Assays for Detection of Chikungunya and O’nyong-nyong Viruses in Mosquitoes

“…The molecular diagnosis of CHIKV has only been evaluated for human samples [16][17][18][19][20][21][22][23] and no real-time RT-PCR assay exists for detecting ONNV present in human or mosquito F igure 1. Detection of CHIKV in infected mosquitoes using real-time reverse transcription-polymerase chain reaction.…”

“…Several groups have developed real-time RT-PCR assays for the molecular diagnosis of CHIK involving human samples. [16][17][18][19][20][21][22][23] However, these assays have not been evaluated for detecting CHIKV RNA in infected mosquitoes. Additionally, no real-time RT-PCR assay exists for detecting ONNV RNA in human or mosquito samples.…”