Molecular and serological detection of bovine babesiosis in Indonesia

Guswanto, Azirwan; Allamanda, Puttik; Mariamah, Euis Siti; Sodirun, Sodirun; Wibowo, Putut Eko; Indrayani, Liliek; Nugroho, Rudi Harso; Wirata, I Ketut; Jannah, Nur; Dias, Lepsi Putri; Wirawan, Hadi Purnama; Yanto, Rochmadi; Tuvshintulga, Bumduuren; Sivakumar, Thillaiampalam; Yokoyama, Naoki; Igarashi, Ikuo

doi:10.1186/s13071-017-2502-0

Cited by 34 publications

(42 citation statements)

References 36 publications

(50 reference statements)

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“…The agreement between the single-ICT and the dual-ICT kappa score was comparable to that previously recorded by Guswanto et al [31]. On the other hand, a comparison of the results of either single-ICT or dual-ICT test with ELISA showed slight agreement to detect babesiosis, contrary to the good agreement reported by Guswanto et al [31]. This detected disparity raises concerns about low sensitivity, specificity, and cross-reactivity, especially in countries where the disease under investigation is endemic [46].…”

Section: Discussionsupporting

confidence: 89%

Section: Discussionsupporting

confidence: 87%

“…In 2008, Neilsen et al [42] developed a lateral flow assay targeting the major surface protein 5 (Msp-5) to detect A. marginale, while Kim et al [27] developed and tested a single and dual assay targeting the BbigRAP1/CT17 and BbovMSA-2c proteins for the detection of B. bigemina and B. bovis, respectively. In subsequent studies, Guswanto et al [31] combined BbovSBP-4 and BbigRAP1/CT17 to screen babesiosis among samples in Indonesia. In the present study, we used the latter to diagnose B. bigemina and B. bovis infections among cattle samples collected from Uganda.…”

Section: Discussionmentioning

confidence: 99%

“…In comparison, the BbovSBP-4 showed superior antigenic characteristics since it is released at the point when merozoites egress from the red blood cell [27][28][29][30]. On the other hand, the C-terminal truncated rhoptry-associated protein 1 (BbigRAP1/CT17) was tested and used successfully to detect B. bigemina infection [27,31]. Later, Kim et al [27] combined the BbigRAP1/CT17 and B. bovis MSA-2c to develop dual ICT strips, but achieved a low viability.…”

Section: Introductionmentioning

confidence: 99%

“…Later, Kim et al [27] combined the BbigRAP1/CT17 and B. bovis MSA-2c to develop dual ICT strips, but achieved a low viability. Comparatively, Guswanto et al [31] succeeded by combining BbovSBP-4 and BbigRAP1/CT17 and used the new dual ICT to diagnose B. bovis and B. bigemina in cattle samples collected from Indonesia. In this study, ICT strips using BbigRAP1/CT17 and BbovSBP-4 were prepared for serological investigation of B. bigemina and B. bovis in cattle samples collected from Uganda.…”

Section: Introductionmentioning

confidence: 99%

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Assessing the Immunochromatographic Test Strip for Serological Detection of Bovine Babesiosis in Uganda

et al. 2020

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In Uganda, bovine babesiosis continues to cause losses to the livestock industry because of shortages of cheap, quick, and reliable diagnostic tools to guide prescription measures. In this study, the presence of antibodies to Babesia bigemina and Babesia bovis in 401 bovine blood samples obtained from eastern and central areas of Uganda were detected using enzyme-linked immunosorbent assays (ELISAs) and immunochromatographic test strips (ICTs). The ELISA and ICT test used targeted the B. bigemina C-terminal rhoptry-associated protein (RAP-1/CT17) and B. bovis spherical body protein-4 (SPB-4). Using ELISA, single-ICT and dual-ICT, positive samples for B. bovis were detected in 25 (6.2%), 17 (4.3%), and 14 (3.7%) samples respectively, and positive samples for B. bigemina were detected in 34 (8.4%), 27 (6.7%), and 25 (6.2%), respectively. Additionally, a total of 13 animals (3.2%) had a mixed infection. The correlation between ELISA and single-ICT strips results revealed slight agreement with kappa values ranging from 0.088 to 0.191 between both methods, while the comparison between dual-ICT and single-ICT results showed very good agreement with kappa values >0.80. This study documented the seroprevalence of bovine babesiosis in central and eastern Uganda, and showed that ICT could, after further optimization, be a useful rapid diagnostic test for the diagnosis of bovine babesiosis in field settings.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Assessing the Immunochromatographic Test Strip for Serological Detection of Bovine Babesiosis in Uganda

et al. 2020

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Cross‐sectional survey of cattle haemopathogens in Constantine, Northeast Algeria

Foughali

Ziam

Aiza

et al. 2021

Veterinary Medicine & Sci

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Piroplasmosis and anaplasmosis are non-contagious vector-borne diseases transmitted by several haematophagous arthropods.Bovine tropical theileriosis is a protozoan disease caused by Theileria annulata transmitted by ticks of the genus Hyalomma, affecting lymphocyte and then red blood cells. (Bilgic et al., 2019;Dolan, 1989;Nourollahi-fard et al., 2015). Babesiosis is haemoprotozoan diseases caused by the presence and multiplication of Babesia spp. in erythrocytes (Bock et al., 2004;Bouattour et al., 2004). They are transmitted by several ixodid ticks, mainly Rhipicephalus (Boophilus) (Barré & Camus, 1983;Uilenberg, 2006). Babesia divergens is transmitted by Ixodes ricinus (Zintl et al., 2003) but was never reported in Algeria.Babesia bovis infection is characterised by fever, icterus, anaemia,

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Molecular detection of Babesia and Theileria from crossbred cattle in Sirajganj and Rangpur districts of Bangladesh

Hossain

Raut

Singh

et al. 2022

Veterinary Medicine & Sci

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Background Babesia and Theileria are potential threats to the livestock industry, causing considerable economic losses. These tick‐borne blood parasites are more prevalent in crossbred cattle than local cattle in Bangladesh. Objectives To confirm the species of Babesia and Theileria in crossbred cattle from the northern part of Bangladesh using conventional and molecular tools. Methods A total of 385 crossbred cattle blood samples were subjected to DNA extraction and PCR. For molecular detection, B. bigemina rhoptry‐associated protein 1a, B. bovis spherical body protein‐4, and Theileria spp. 18S rRNA were used as the marker genes. Results Using PCR, only 72 (18.7%) samples were found piroplasm positive, of which 12.2% Theileria, 4.7% Babesia, and 1.8% mixed infections. Both Babesia (7.3%), Theileria (7.7%) and mixed (2.8%) infections were detected in Sirajganj, and only Theileria (20.4%) was detected in Rangpur district. By PCR and nPCR we detected B. bigemina and T. annulata in Sirajganj district, and Theileria sp. in Rangpur district. The target gene sequences of isolated pathogens confirmed B. bigemina and T. annulata, and Theileria sp from these samples. Blood smears of all samples were also examined microscopically for Babesia and/or Theileria spp. and 14.3% of samples were found positive, of which 5.9% Babesia and 8.3% Theileria. Generally, the pathogens detected in Sirajgang and Rangpur were genetically related to South Asia, particularly South East Asian isolates. Conclusions These findings provide information for a better understanding of the epidemiology of Babesia and Theileria as well as to improve the approaches for diagnosis and control of tick‐borne diseases in Bangladesh.

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Molecular and serological detection of bovine babesiosis in Indonesia

Cited by 34 publications

References 36 publications

Assessing the Immunochromatographic Test Strip for Serological Detection of Bovine Babesiosis in Uganda

Assessing the Immunochromatographic Test Strip for Serological Detection of Bovine Babesiosis in Uganda

Cross‐sectional survey of cattle haemopathogens in Constantine, Northeast Algeria

Molecular detection of Babesia and Theileria from crossbred cattle in Sirajganj and Rangpur districts of Bangladesh

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