Molecular and Serological Assessment of Chronic Parvovirus B19 among Chronic Hemolytic Anemia Children

Makled, Amal F.; Salama, Ahmed A; El-Hawy, Mahmoud A.

doi:10.12816/0046268

Cited by 2 publications

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“…The second round primer was 5′-CAAAAGCATGTGGAGTGAGG-3′ and 5′-CC TTATAATGGTGCTCTGGG-3′ to give a product of 104 bp. About 10 µl of second round PCR products were analyzed by 2% agarose gel electrophoresis then bands were visualized after ethidium bromide staining 14, 15 .…”

Section:  Nested Pcr For Parvovirus B19-dna Detectionmentioning

confidence: 99%

Molecular and Serological Study of Parvovirus B19 Infection among Rheumatoid Arthritis Patients at Menoufia University Hospital

Elkhyat

Saied

Galbat

et al. 2022

Egyptian Journal of Medical Microbiology

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Background: Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease. Conflicting reports are available about the association between human parvovirus B19 (B19) infection and RA. Most studies were done in European and Asian countries, but only few studies were performed in Africa. Objectives: This study aimed to investigate the seroprevalence of parvovirus B19 infection in RA patients compared with healthy controls and to search for possible association of B19 viremia with disease activity and severity. Methodology: This case-control study was conducted on 50 RA patients who fulfilled the 2010 American College of Rheumatology/European League against Rheumatism classification criteria for rheumatoid arthritis and 30 matched healthy controls. All participants were examined for parvovirus B19 infection by serological detection of anti-B19 IgM and IgG by ELISA and B19 DNA by nested PCR. Results: Parvovirus B19 DNA was detected in 17 (34%) of patients While controls were (6.7%) with a significant difference (0.005). There was significant difference between patients and controls (P=0.007) regarding IgG anti-B19 antibody but not anti-B19 IgM (P =0.59). There was a significant association between B19 viremia and all activity parameters. B19 positive patients had higher levels of ESR and CRP, higher DAS28 scores and more affected joints than B19 negative patients with statistically significant differences. B19 positive patients had significantly higher levels of RF and anti-CCP. Furthermore, B19 positive patients were more likely to have joint erosion. Conclusion: This study revealed that parvovirus B19 infection may play a role in the aetiopathogenesis of RA.

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Section:  Nested Pcr For Parvovirus B19-dna Detectionmentioning

confidence: 99%

Molecular and Serological Study of Parvovirus B19 Infection among Rheumatoid Arthritis Patients at Menoufia University Hospital

Elkhyat

Saied

Galbat

et al. 2022

Egyptian Journal of Medical Microbiology

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“…The transmission of parvovirus B19 occurs mainly via respiratory droplets but it can also spread by contaminated blood, organ transplantation and from mother to fetus 6 .The high-risk period for spread is early in the acute phase of infection. Infected patients are most contagious during this phase &patients can be asymptomatic or present with non-specific flu-like illness 7 . While the typical symptoms attributed to PV-B19 infection can be the results of other infective processes 8 .…”

Section: Introductionmentioning

confidence: 99%

Detection of Human Parvovirus B19 Infection in Cases of Systemic Lupus Erythematosus Attending Benha University Hospitals

Shafik

Elgazar

El-Nahal

et al. 2022

Egyptian Journal of Medical Microbiology

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Background: Systemic lupus erythematosus is an autoimmune disease. Parvovirus B19 infection can break tolerance to self-DNA and promote pathogenesis of autoimmunity and might induce either idiopathic SLE in a person who is genetically susceptibl or it might induce a SLE-like picture. Objectives: Detection of PV-B19 DNA and it ' s IgM and IgG antibodies in the serum of SLE patients and in apparently healthy volunteer in Benha University Hospitals. Methodology: The study was conducted on 60 subjects classified into 2 groups: Group I: Including 40 SLE patients fulfilling SLICC Classification Criteria , 20 of them were in exacerbation state and the other 20 cases were in remission state and Group II: Including 20 age and sex matched apparently healthy volunteers serve as a control group . All patients were subjected to full history taking, clinical examination and laboratory investigations. PV-B19 IgM and IgG were measured using anti PV-B19 ELISA kits and PV-B19 DNA was detected by using real time polymerase chain reaction (PCR). Results: PV-B19 DNA was detected in 9 cases (45%) with active disease, Out of the 9 patients with PV-B19 DNA, only 1 had positive PCR results alone , 1 had positive PCR and IgM results and 7 had positive PCR, IgM and IgG results. on the other hand, PV-B19 DNA was detected in only 1 case (5%) out of 20 remittent cases (p=0.003). Active cases were significantly associated with higher frequency of IgM and IgG when compared to remittent cases (p=0.010, 0.020, respectively).There was significant differences in SLEDAI between active and remitted cases (p<0.001). Conclusion: Combined use of PCR and ELISA are needed for accurate diagnosis of PV-B19 infection.

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Molecular and Serological Assessment of Chronic Parvovirus B19 among Chronic Hemolytic Anemia Children

Cited by 2 publications

References 0 publications

Molecular and Serological Study of Parvovirus B19 Infection among Rheumatoid Arthritis Patients at Menoufia University Hospital

Molecular and Serological Study of Parvovirus B19 Infection among Rheumatoid Arthritis Patients at Menoufia University Hospital

Detection of Human Parvovirus B19 Infection in Cases of Systemic Lupus Erythematosus Attending Benha University Hospitals

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