Molecular and serological assessment of parvovirus B-19 infection in Egyptian children with sickle cell disease

Makhlouf, Manal Mohamed; Elwakil, Sahar; Ibrahim, Nihal Salah

doi:10.1016/j.jmii.2015.10.016

Cited by 9 publications

(9 citation statements)

References 20 publications

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“…Among 64 haemoglobin SS (sickle cell anaemia) and 36 haemoglobin AS (sickle cell trait) children with IgG positive titers, 24 had evidence of parvovirus B19 DNA as detected by polymerase chain reaction (PCR). 4 Patients with viremia were reported to have a lower baseline haemoglobin than those with a previous history of parvovirus B19 infection denoted by serological markers but no active viremia. 4 Although epidemiology of parvovirus B19 seropositivity in SCD has been well documented, there are few studies that have assessed the presence of persistent DNAemia and if there is an association to RBC transfusion.…”

Section: Discussionmentioning

confidence: 99%

“…In a cross‐sectional study, Makhlouf et al showed that parvovirus B19 DNAemia appears to be common in SCD. Among 64 haemoglobin SS (sickle cell anaemia) and 36 haemoglobin AS (sickle cell trait) children with IgG positive titers, 24 had evidence of parvovirus B19 DNA as detected by polymerase chain reaction (PCR) . Patients with viremia were reported to have a lower baseline haemoglobin than those with a previous history of parvovirus B19 infection denoted by serological markers but no active viremia …”

Section: Introductionmentioning

confidence: 99%

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Parvovirus B19 infection in sickle cell disease: An analysis from the Centers for Disease Control haemoglobinopathy blood surveillance project

Majumdar

Bean

Staercke

et al. 2020

Transfusion Medicine

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Summary Objective In the multicentre Haemoglobinopathy Blood Surveillance Project, to evaluate the seroprevalence of parvovirus B19 and DNA viral load in sickle cell disease (SCD). Background Although the epidemiology of parvovirus B19 seropositivity in SCD has been well documented, there are few studies that have assessed possible persistent parvovirus DNAemia and associated risk factors including blood transfusion. Methods A qualitative analysis of parvovirus B19 serology using ELISA and quantitative parvovirus B19 DNA by RT‐PCR was performed in patients with SCD. Results Of 322 patients, 113 (35%) were parvovirus IgG positive and 119 (37%) were IgM positive at enrolment. The prevalence of IgG positivity increased with age. 71/322 (22%) were parvovirus DNA positive at enrolment with a mean viral load of 15 227 ± 55 227 SD. (range 72‐329 238 IU/mL). Patients who were positive for parvovirus B19 DNA received a significantly higher red blood cell transfusion volume in the prior year compared to patients who were negative (mean RBC volume = 8310 mL vs 5435 mL, respectively; P = .0073). Seventy‐seven patients had follow‐up testing approximately 1 year after enrolment and 11/28 (39%) patients had persistently positive IgM. Conclusion Further studies are needed to better understand the natural history of parvovirus B19 infection in SCD especially in relation to RBC transfusion as a risk factor, as well as disease outcome and severity.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Parvovirus B19 infection in sickle cell disease: An analysis from the Centers for Disease Control haemoglobinopathy blood surveillance project

Majumdar

Bean

Staercke

et al. 2020

Transfusion Medicine

View full text Add to dashboard Cite

show abstract

“…In another study in Egypt, Makhlouf et al assessed the serological and molecular prevalence of the parvovirus B19 in 100 SCD patients and 60 controls. The results revealed that 30% of SCD patients were positive for the parvovirus B19 IgM and DNA, while 24% had positive IgG and DNA by nested-PCR [25].…”

Section: Discussionmentioning

confidence: 97%

A literature review on the parvovirus B19 infection in sickle cell anemia and β-thalassemia patients

et al. 2020

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Background Parvovirus B19 is the causative agent for erythema infectiosum, and also as a potentially life-threatening infectious agent, it is mainly presented in high erythrocyte turnover patients. Sickle cell disease (SCD) is an inherited monogenic hematological disorder resulting from the mutations in the hemoglobin β-chain gene. Thalassemia is a hereditary hematological syndrome that happens in consequence of deficiencies in the production of one or more globin chains. We summarize current knowledge about the prevalence rates of the parvovirus B19 infection in sickle cell anemia and thalassemia patients. Methods Several online databases were searched including, Scopus, EMBASE, Web of Science, Google Scholar, and PubMed, which were performed amidst 2009–2019 by using distinct keywords: “Thalassemia,” “Parvovirus,” “Anemia,” “Sickle cell anemia,” “parvoviridae,” “parvoviridae infection,” and “parvovirus B19.” Results Search results indicated 4 and 7 studies for the prevalence of the parvovirus B19 in β-thalassemia and SCD, respectively. Among the β-thalassemia patients, the B19V seroprevalence for IgG and IgM were ranged from 18.2–81% and 14.5–41.1%, respectively; meanwhile, B19V DNA positively results was 4–15.3%. Moreover, in the SCD group, the extent of B19V IgG was varied from 37.6 to 65.9% and that of IgM was in a range of 2.9–30%, and the DNA detection rate was 4–54%. Conclusion B19V seroprevalence changes in several conditions including, different epidemiological features, socio-economic status, and overpopulation. Age can expand the incidence of anti-B19V IgG/IgM in SCD and beta-thalassemia patients. Reinfection and diverse genotypes are relevant factors in the seroprevalence of B19v. The patients’ immunological-hematological station and higher abundance of transfusions can affect the B19V seroprevalence in SCD and beta-thalassemia group. Further investigations in this field could be suggested to better understand the virus distribution in this susceptible population of patients.

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“…The virus displays remarkable tropism for human erythroid progenitor cells. In immunocompromised hosts unable to neutralize the antibody, B19 V replicates in erythrocyte precursors e ciently and preferentially, and this infection may persist and lead to pure red cell anemia and other comorbidities [3]. It has been estimated that the peak incidence of infection occurs in children between the ages of 6 and 14 years [4].…”

Section: Discussionmentioning

confidence: 99%

Aplastic Crisis Induced by Human Parvovirus B19 Infection in a Previously Healthy Adult Male

Cheng

Liu

et al. 2020

Preprint

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Background: Human parvovirus B19 (B19 V) induced aplastic crisis is very rare and in this paper, we describe a healthy adult male suddenly developed acute aplastic crisis induced by B19 V was found to have hereditary spherocytosis (HS).Case presentation: A 31-year-old male presented with fever, general fatigue and dizziness. The complete blood counts showed severe anemia. Folate levels, glucose-6-phosphate dehydrogenase levels, the direct Coombs' test and osmotic fragility tests were all normal. And B19 V infection was proven both by the detection of B19 V DNA using PCR and the presence of B19 V IgM by serology and the patient improved following six units of packed red blood cells and antiviral treatment.Conclusions: This is the first report to describe a case with B19 V, EBV and CMV co-infection diagnosed in a patient with HS in China and DNA test of B19 V is recommended in acute aplastic crisis diseases.

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Molecular and serological assessment of parvovirus B-19 infection in Egyptian children with sickle cell disease

Abstract: PB-19 infection is detected at high rates among Egyptian children with sickle cell disease and it may result in severe anemia. So, PB-19 must be suspected and screened for in such group of patients.

Cited by 9 publications

References 20 publications

Parvovirus B19 infection in sickle cell disease: An analysis from the Centers for Disease Control haemoglobinopathy blood surveillance project

Parvovirus B19 infection in sickle cell disease: An analysis from the Centers for Disease Control haemoglobinopathy blood surveillance project

A literature review on the parvovirus B19 infection in sickle cell anemia and β-thalassemia patients

Aplastic Crisis Induced by Human Parvovirus B19 Infection in a Previously Healthy Adult Male

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