Molecular and restriction fragment length polymorphism analysis of canine parvovirus 2 (CPV-2) in dogs in southeast Anatolia, Turkey

Abstract

Canine parvovirus-2 (CPV-2) is the aetiological agent of an infectious viral disease of dogs, characterised by diarrhoea and vomiting. Mutations of the CPV-2 genome have generated new variants circulating worldwide. This article reports the molecular analysis of CPV-2 variants collected in the dog population in southeast Anatolia, Turkey. Twenty blood samples previously taken for the laboratory diagnosis of dogs with suspected parvovirus were screened for CPV-2 by polymerase chain reaction (PCR). Of the 20 samples, 18 tested positive for CPV-2. Partial VP2 gene sequencing and restriction fragment length polymorphism (RFLP) analysis revealed CPV-2a (n = 1), CPV-2b (n = 16) and CPV-2c (n = 1) variants. Phylogenetic analysis based on the partial length VP2 gene showed that CPV-2b (n = 15) variants showed sequences clustering separately in the phylogenetic tree. The CPV-2c sample was phylogenetically related to Chinese strains and Indonesia strain, whereas the CPV-2a sample was phylogenetically related to the Portuguese strain. These results, which are the first to demonstrate the presence of CPV-2c in the dog population of southeast Anatolia, Turkey, indicate that CPV-2a/2b/2c variants co-exist in Turkey’s dog population.

Detecting Canine Distemper Virus From Ocular Swab and Blood Samples of Dogs by Real-Time Rt-PCR Method

“…In Turkey, its epidemiological presence has been revealed by serological tests such as ELISA (Saltık and Kale 2020) and neutralization tests (Gencay et al 2004). When we look at the recent studies, even though there are many studies in our country for diagnosing respiratory and digestion system infections (Avci et al 2016, Dinçer 2017, Polat et al 2019, Timurkan et al 2021), those about CDV are quite limited (Oğuzoğlu et al 2018). During these limited amounts of studies, CDV genome presence has been revealed by RT-PCR method.…”

Molecular characterization of canine coronaviruses: an enteric and pantropic approach

“…The importance of emerging (SARS/MERS) and re-emerging (CPV-2c or COVID-19) infections is currently increasing [2,23,24]. Therefore, the genetic characteristics of these infectious agents need to be examined frequently due to emerging new types, changes in pathogenesis, and the dynamism of immune escape mechanisms.…”

The analysis of canine parvovirus strains based on the sequence VP-2 GENE

“…Розвиток науки сприяє удосконаленню засобів діагностики та профілактики інфекційних захворювань взагалі та вірусних хвороб собак зокрема. З цією метою широко використовують молекулярно-генетичні методи (Csagola, Varga, Lorincz, & Tuboly, 2014;Hao et al, 2019;Matsuda, 2017;Zhou, Zeng, Zhang, & Li, 2017 (Miranda, & Thompson, 2016;Polat, Sahan, Aksoy, Timurkan, & Dincer, 2019;Sun et al, 2019;Tucciarone et al, 2018) В Україні вакцинація парвовірусного ентериту собак -один із головних заходів боротьби із захворюванням.…”