Molecular and physiological analysis of indole-3-acetic acid degradation in Bradyrhizobium japonicum E109

Torres, Daniela; Mongiardini, Elías J.; Donadío, Florencia; Donoso, Raúl; Recabarren‐Gajardo, Gonzalo; Gualpa, José Luis; Spaepen, Stijn; Defez, Roberto; López, Gastón; Bianco, Carmen; Cassán, Fabricio

doi:10.1016/j.resmic.2021.103814

Cited by 20 publications

(12 citation statements)

References 40 publications

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“…The main classes of phytohormones are auxins, abscisic acids, gibberellins, ethylene, and cytokinins 1 , 2 . Indole 3-acetic acid ( IAA ) is an auxin which has an important role in various plant physiological processes, e.g., cell elongation, division, and differentiation, flowering, fruit development 1 – 7 .…”

Section: Introductionmentioning

confidence: 99%

Effects of process factors on performances of liquid membrane-based transfer of indole-3-acetic acid

Diaconu

Pârvulescu

Topală

et al. 2021

Sci Rep

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The paper has aimed at studying the transfer of indole 3-acetic acid (IAA) from a feed aqueous solution to a stripping aqueous solution of NaOH using a chloroform bulk liquid membrane and trioctylamine (TOA) as a ligand (L). Initial molar concentrations of IAA in the feed phase, cIAA,F0 (10–4–10–3 kmol/m3), of TOA in the membrane phase, cL,M0 (10–2 and 10–1 kmol/m3), and of NaOH in the stripping phase, cNaOH,S0 (10–2 and 1 kmol/m3), were selected as process factors. Their effects on the final values of IAA concentration in the feed phase (cIAA,Ff) and stripping solution (cIAA,Sf), extraction efficiency (EF), distribution coefficient (KD), and recovery efficiency (ER) were quantified using multiple regression equations. Regression coefficients were determined from experimental data, i.e., cIAA,Ff,ex = 0.02–1 × 10–4 kmol/m3, cIAA,Sf,ex = 0.22–2.58 × 10–3 kmol/m3, EF,ex = 90.0–97.9%, KD,ex = 9.0–46.6, and ER,ex = 66.5–94.2%. It was found that cIAA,F0 had the most significant positive effect on cIAA,Ff and cIAA,Sf, whereas cNaOH,S0 had a major positive effect on EF, KD, and ER. A deterministic model based on mass transfer of IAA was developed and its parameters, i.e., mass transfer coefficient of IAA-L complex in the liquid membrane (0.82–11.5 × 10–7 m/s) and extraction constant (1033.9–1779.7 m3/kmol), were regressed from experimental data. The effect of cL,M0 on both parameters was significant.

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Section: Introductionmentioning

confidence: 99%

Effects of process factors on performances of liquid membrane-based transfer of indole-3-acetic acid

Diaconu

Pârvulescu

Topală

et al. 2021

Sci Rep

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“…Recent studies suggested that many non-symbiotic Bradyrhizobium species are ecologically important for the soil microbiota and such ecotypes dominate the coniferous forest soil (VanInsberghe et al 2015;Jones et al 2016;Ormeño-Orrillo and Martínez-Romero 2019). Moreover, Bradyrhizobium strains were reported previously to degrade auxin (Egebo, Nielsen, and Jochimsen 1991;Jensen et al 1995;Torres et al 2021), whose level plays an important role in plant resilience (Finkel et al 2020). Thus, it is tempting to speculate that these newly observed beneficial Bradyrhizobiaceae strains may have plant growth promoting properties including auxin degradation, which complement the absence of Variovorax in this soil.…”

Section: Discussionmentioning

confidence: 99%

Identification of beneficial and detrimental bacteria that impact sorghum responses to drought using multi-scale and multi-system microbiome comparisons

Berry

Veley

et al. 2021

Preprint

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Background: Drought is a major abiotic stress that limits agricultural productivity. Previous field-level experiments have demonstrated that drought decreases microbiome diversity in the root and rhizosphere and may lead to enrichment of specific groups of microbes, such as Actinobacteria. How these changes ultimately affect plant health is not well understood. In parallel, model systems have been used to tease apart the specific interactions between plants and single, or small groups of microbes. However, translating this work into crop species and achieving increased crop yields within noisy field settings remains a challenge. Thus, the next scientific leap forward in microbiome research must cross the great lab-to-field divide. Toward this end, we combined reductionist, transitional and ecological approaches, applied to the staple cereal crop sorghum to identify key beneficial and detrimental, root associated microbes that robustly affect drought stressed plant phenotypes. Results: Fifty-three bacterial strains, originally characterized for association with Arabidopsis, were applied to sorghum seeds and their effect on root growth was monitored for seven days. Two Arthrobacter strains, members of the Actinobacteria phylum, caused root growth inhibition (RGI) in Arabidopsis and sorghum. In the context of synthetic communities, strains of Variovorax were able to protect both Arabidopsis and sorghum from the RGI caused by Arthrobacter. As a transitional system, we tested the synthetic communities through a 24-day high-throughput sorghum phenotyping assay and found that during drought stress, plants colonized by Arthrobacter were significantly smaller and had reduced leaf water content as compared to control plants. However, plants colonized by both Arthrobacter and Variovorax performed as well or better than control plants. In parallel, we performed a field trial wherein sorghum was evaluated across well-watered and drought conditions. Drought responsive microbes were identified, including an enrichment in Actinobacteria, consistent with previous findings. By incorporating data on soil properties into the microbiome analysis, we accounted for experimental noise with a newly developed method and were then able to observe that the abundance of Arthrobacter strains negatively correlated with plant growth. Having validated this approach, we cross-referenced datasets from the high-throughput phenotyping and field experiments and report a list of high confidence bacterial taxa that positively associated with plant growth under drought stress. Conclusions: A three-tiered experimental system connected reductionist and ecological approaches and identified beneficial and deleterious bacterial strains for sorghum under drought stress.

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“…The 1:1 treatment was chosen because it had the greatest values of EPS production, bacterial biomass, and IAA degradation for which AbAz39 is responsible (Table S1). These parameters were selected with reference to previous studies published by our laboratory (Torres et al 2018;2021), in which we con rmed that exogenous IAA addition to pure BjE109 cultures triggered physiological changes in the bacterium, such as the ability to increase its biomass and produce EPS, both are advantages on the rhizobia cell survival on soybean seeds and on its symbiotic performance. We also obsserved that BjE109 can degrade IAA when is added to the culture medium through the action of a 3-phenylpropanoate dioxygenase-like enzyme (subunit alpha and beta) within a cluster named iac (Torres et al 2018;2021).…”

Section: Discussionmentioning

confidence: 99%

“…Quanti cation of IAA was performed by spectrophotometry (Glickmann and Dessaux 1995) and con rmed by HPLC following Torres et al (2021). Aliquots of 1000 µl of bacterial culture were centrifuged at 11,300 g for 10 min.…”

Section: Iaa Quanti Cationmentioning

confidence: 99%

The Interaction between Bradyrhizobium Japonicum E109 and Azospirillum Brasilense Az39 Improves Bradyrhizobium-Soybean Symbiosis: The Secrets Behind Co-Inoculation

Torres

Donadío

López

et al. 2021

Preprint

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Aims The aim of this work was evaluating the interaction between two of the most widely used strains for soybean inoculation in Argentina, B. japonicum E109 (BjE109) and A. brasilense Az39 (AbAz39). Methods Five treatments were performed: (i) uninoculated seeds; (ii) seeds inoculated with BjE109; (iii) seeds inoculated with AbAz39; (iv) seeds co-inoculated with BjE109 and AbAz39 in a 1:1 proportion (BjE109 + AbAz39) at the seeds sowing and (v) seeds inoculated with a 1:1 proportion of BjE109 and AbAz39 (BjE109-AbAz39) 24 h before seeds sowing. Each treatment was assessed through a seed recovery assay, glasshouse assays and field assays. Results The combination between the two strains improved the ability of BjE109 to survive on soybean seeds after inoculation partially explained by AbAz39’s capacity to produce diverse biologically active molecules. As a result of the greater rhizobial survival on seeds the nodulation values and symbiosis parameters like nodule number, size and biomass and nodulation percentage also increased. In agreement with these observations, combining BjE109 and AbAz39 at strains the grain yield under field conditions were 13,3 and 17,3% greater than single BjE109 inoculation. Conclusions These results here show that the pre-culture combining BjE109 and AbAz39 before the inoculation to the soybean seeds has benefits in plant nodulation and hence production, more than individual inoculation with BjE109 or AbAz39, or the immediate co-inoculation of both strains.

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Molecular and physiological analysis of indole-3-acetic acid degradation in Bradyrhizobium japonicum E109

Cited by 20 publications

References 40 publications

Effects of process factors on performances of liquid membrane-based transfer of indole-3-acetic acid

Effects of process factors on performances of liquid membrane-based transfer of indole-3-acetic acid

Identification of beneficial and detrimental bacteria that impact sorghum responses to drought using multi-scale and multi-system microbiome comparisons

The Interaction between Bradyrhizobium Japonicum E109 and Azospirillum Brasilense Az39 Improves Bradyrhizobium-Soybean Symbiosis: The Secrets Behind Co-Inoculation

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