Molecular and physical mapping of a barley gene on chromosome arm 1HL that causes sterility in hybrids with wheat

Abstract

Addition of the long arm of barley chromosome 1H (1HL) to wheat causes severe meiotic abnormalities and complete sterility of the plants. To map the barley gene responsible for the 1H-induced sterility of wheat, a series of addition lines of translocated 1H chromosomes were developed from the crosses between the wheat 'Shinchunaga' and five reciprocal translocation lines derived from the barley line St.13559. Examination of the seed fertility of the addition lines revealed that the sterility gene is located in the interstitial 25% region of the 1HL arm. The genetic location of the sterility gene was also estimated by physically mapping sequence-tagged site (STS) markers and simple-sequence repeat (SSR) markers with known map locations. The sterility gene is designated Shw (sterility in hybrids with wheat). Comparison of the present physical map of 1HL with two previously published genetic maps revealed a paucity of markers in the proximal 30% region and non-random distribution of SSR markers. Two inconsistencies in marker order were found between the present physical map and the consensus genetic map of group 1 chromosomes of Triticeae. On the basis of the effects on meiosis and chromosomal location, the relationship of the present sterility gene with other fertility-related genes of Triticeae is discussed.

Molecular and genetic characterization of the S locus in Hordeum bulbosum L., a wild self-incompatible species related to cultivated barley

“…We also mapped two barley markers (Glb1 and mwg943) whose physical locations were determined to be on the long arm of chromosome 1H (Taketa et al 2002), using the 17 recombinants mentioned above. Two recombinants (SMP1-16 and -71) also showed recombination between S and the two markers, suggesting that Glb1 and mwg943 were also mapped to a distance of 0.30 cM from S, the same position as the seven markers (HAS5, etc.…”

“…Of the remaining three markers obtained in the present AMF analysis, HAS 31 and HAS175 appeared to exhibit little sequence polymorphism Künzel et al (2000), respectively. Physical locations of barley markers are after Künzel et al (2000), Taketa et al (2002) and Sourdille et al (2004). 1HS and 1HL indicate short and long arms of chromosome 1H, respectively.…”

Wheat–barley hybridization: the last 40 years

“…Although Tritordeum already exists as the product of the wheat 9 H. chilense combination, and fertile amphiploids have been produced with H. marinum and H. californicum, fertile T. aestivum 9 H. vulgare amphiploids have not yet been developed. Unfortunately the chromosome 1H of H. vulgare carries a gene (Shw) that causes sterility in wheat background (Taketa et al 2002) and it prevents the production of a fertile amphiploid. The development of small barley introgressions in the wheat genetic background has been started, but a much larger quantity of translocations carrying genes responsible for useful traits are needed.…”

Dissecting the maize genome by using chromosome addition and radiation hybrid lines

“…The situation appears similar to the difficulties of generating a disomic euplasmic addition line for Betzes barley chromosome 1H and for its long-arm telosome 1HL in Chinese Spring wheat (25). The difficulties in wheat (26)(27)(28) appear to be caused by the interaction of the gene Shw (sterility in hybrid with wheat) with the wheat background causing sterility. However, the sterility was alleviated by the simultaneous addition of monosomic or disomic chromosome 6H to the 1H addition (29).…”