Molecular and phylogenetic characterization of potentially toxic cyanobacteria in Tunisian freshwaters

Fathalli, Afef; Jenhani, Amel Ben Rejeb; Moreira, Cristiana; Welker, Martin; Romdhane, Mohamed Salah; Antunes, Agostinho; Vasconcelos, Vı́tor

doi:10.1016/j.syapm.2010.12.003

Cited by 38 publications

(28 citation statements)

References 40 publications

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“…Previous phylogeographic studies have suggested that Cylindrospermopsis strains were separated into three distinct groups, namely, strains from Australia, Europe, and America, with African strains and the former two groups being closely related (71)(72)(73)(74). However, inconsistent phylogenetics have been observed for Tunisian and Spanish strains clustered into the America group (75,76), and for clade II with strains from China, Australia, and Brazil without geographical separation. The present hypotheses suggested that the worldwide dispersion of Cylindrospermopsis originated from the tropical zones of Africa and Australia (77) or the warm refuge areas of each continent (73).…”

Section: As Displayed Inmentioning

confidence: 96%

Sporadic Distribution and Distinctive Variations of Cylindrospermopsin Genes in Cyanobacterial Strains and Environmental Samples from Chinese Freshwater Bodies

Jiang

Xiao

et al. 2014

Appl Environ Microbiol

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e Increasing reports of cylindrospermopsins (CYNs) in freshwater ecosystems have promoted the demand for identifying all of the potential CYN-producing cyanobacterial species. The present study explored the phylogenetic distribution and evolution of cyr genes in cyanobacterial strains and water samples from China. Four Cylindrospermopsis strains and two Raphidiopsis strains were confirmed to produce CYNs. Mutant cyrI and cyrK genes were observed in these strains. Cloned cyr gene sequences from eight water bodies were clustered with cyr genes from Cylindrospermopsis and Raphidiopsis (C/R group) in the phylogenetic trees with high similarities (99%). Four cyrI sequence types and three cyrJ sequence types were observed to have different sequence insertions and repeats. Phylogenetic analysis of the rpoC1 sequences of the C/R group revealed four conserved clades, namely, clade I, clade II, clade III, and clade V. High sequence similarities (>97%) in each clade and a divergent clade IV were observed. Therefore, CYN producers were sporadically distributed in congeneric and paraphyletic C/R group species in Chinese freshwater ecosystems. In the evolution of cyr genes, intragenomic translocations and intergenomic transfer between local Cylindrospermopsis and Raphidiopsis were emphasized and probably mediated by transposases. This research confirms the existence of CYN-producing Cylindrospermopsis in China and reveals the distinctive variations of cyr genes.

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Section: As Displayed Inmentioning

confidence: 96%

Sporadic Distribution and Distinctive Variations of Cylindrospermopsin Genes in Cyanobacterial Strains and Environmental Samples from Chinese Freshwater Bodies

Jiang

Xiao

et al. 2014

Appl Environ Microbiol

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“…Other studies on Microcystis used other genetic markers and were carried out in other locations. These include the USA [66], Kenya and Uganda [54], Russia [67], Tunisia [68,69] and Japan [70,71]. In all, a high level of genetic diversity was observed with a high number of genotypes having been found.…”

Section: Phylogeny Studies In Cyanobacteria and Cyanotoxinsmentioning

confidence: 99%

“…[77] found that the genetic population of Nodularia in the Baltic Sea is heterogeneous with more than one genotype having been found. In C. raciborskii , a low genetic variability was observed when comparing isolates from several locations in Tunisia that appeared in a unique cluster with the use of the rpoC1 marker [69,78]. …”

Section: Phylogeny Studies In Cyanobacteria and Cyanotoxinsmentioning

confidence: 99%

Phylogeny and Biogeography of Cyanobacteria and Their Produced Toxins

2013

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Phylogeny is an evolutionary reconstruction of the past relationships of DNA or protein sequences and it can further be used as a tool to assess population structuring, genetic diversity and biogeographic patterns. In the microbial world, the concept that everything is everywhere is widely accepted. However, it is much debated whether microbes are easily dispersed globally or whether they, like many macro-organisms, have historical biogeographies. Biogeography can be defined as the science that documents the spatial and temporal distribution of a given taxa in the environment at local, regional and continental scales. Speciation, extinction and dispersal are proposed to explain the generation of biogeographic patterns. Cyanobacteria are a diverse group of microorganisms that inhabit a wide range of ecological niches and are well known for their toxic secondary metabolite production. Knowledge of the evolution and dispersal of these microorganisms is still limited, and further research to understand such topics is imperative. Here, we provide a compilation of the most relevant information regarding these issues to better understand the present state of the art as a platform for future studies, and we highlight examples of both phylogenetic and biogeographic studies in non-symbiotic cyanobacteria and cyanotoxins.

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“…Falconer, 2005;Fathalli et al, 2011). Our aims were (1) to develop a new method for discriminating between genotypes producing MCs and those that do not, using a mcyD gene fragment and Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) to detect MCs; (2) to test the method using strains of Microcystis currently held by the Cyanobacteria and Algae Culture Collection of The Institute of Biological Research, Cluj-Napoca, Romania (AICB); and (3) examine the suitability of the assay for characterizing environmental samples.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of microcystin-producing cyanobacteria from Romanian fresh waters

Drugă

Welker

Sesărman

et al. 2013

European Journal of Phycology

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In this study the molecular analysis of 24 potentially toxic cyanobacterial strains of genus Microcystis isolated from Romania is presented. The toxic potential was assessed by Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS), which indicated that five out of the 24 strains are able to produce microcystins. Beside this, the strains were shown to produce various peptides, including aeruginosins, microginins or cyanopeptolins. Identification of potentially toxic Microcystis strains by PCR was performed using primers described in known literature. These primers occasionally generated amplification products in non-microcystin-producing strains. New primers were designed, and the amplification of a mcyD gene fragment exclusively in microcystin-producing strains certified the high specificity of these new primers for microcystinproducing cells. All of the five microcystin-producing strains were shown to possess four characteristic parts of the gene cluster responsible for microcystin synthesis (mcyA, mcyB, mcyD and mcyE). The 16S-23S rDNA ITS sequence was used to confirm the taxonomic identification of the strains and their relationship to strains from outside Romania.

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Molecular and phylogenetic characterization of potentially toxic cyanobacteria in Tunisian freshwaters

Cited by 38 publications

References 40 publications

Sporadic Distribution and Distinctive Variations of Cylindrospermopsin Genes in Cyanobacterial Strains and Environmental Samples from Chinese Freshwater Bodies

Sporadic Distribution and Distinctive Variations of Cylindrospermopsin Genes in Cyanobacterial Strains and Environmental Samples from Chinese Freshwater Bodies

Phylogeny and Biogeography of Cyanobacteria and Their Produced Toxins

Molecular characterization of microcystin-producing cyanobacteria from Romanian fresh waters

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