Molecular and phylogenetic analysis of the dengue strains circulating in Odisha, India

Sabat, Jyotsnamayee; Subhadra, Subhra; Thakur, Bhaskar; Panda, Manasee; Panda, Sailendra; Ho, Lal Mohan; Dixit, Sujata; Rathore, Sushil Kumar; Kar, Shantanu Kumar; Dwibedi, Bhagirathi

doi:10.1007/s13337-019-00544-3

Cited by 6 publications

(6 citation statements)

References 16 publications

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“…A study from Odisha confirmed the emergence of CHIKV infection in Odisha in 2006 and its spread was facilitated by the presence of the Aedes vector species affecting a wide geographical area within a short duration of time [15]. This study is the first to report the prevalence of dengue, re- [13]. In this study, 43.8% dengue seropositivity was noted which is in concordance with the results reported by Gupta et al [16].…”

Section: Discussionsupporting

confidence: 90%

“…In most parts of India, occurrence of dengue infection is a regular phenomenon with periodic upsurges and also there is a progressive spread of chikungunya virus from south to north Indian regions in the last decade [11,12]. First dengue outbreak was reported in the state of Odisha in the year 2010 which continued in the subsequent years in the form of epidemic during post monsoon period gradually becoming endemic in nature [13]. India experienced outbreaks due to CHIK infection in 2005 after a long gap [14] whereas Odisha with no history of Chikungunya infection experienced its first epidemic in 2006.…”

Section: Discussionmentioning

confidence: 99%

“…al. [13]. Season is a significant determining factor in the incidence of infectious diseases, mainly arboviral infections, such as dengue, chikungunya, and Zika.…”

Section: Discussionmentioning

confidence: 99%

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Prevalence and trend of emerging and re-emerging arboviral infections in the state of Odisha

et al. 2021

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Newly emerging or re-emerging infections are posing continuous threat to both public health system and clinical care globally. The emergence of infections especially caused by arboviruses can be linked to several mechanisms which include geographical expansion linked to human development and transportation, global warming, enhanced transmission in peridomestic area and close proximity of human habitations to domestic as well as wild animals. The co-circulation of Dengue, Chikungunya and Zika is a matter of public health priority due to the fact that they are transmitted by the same vector as well as increase in the number of reported cases of severe dengue, postchikungunya chronic joint disease and microcephaly related to Zika virus disease. The study was designed to estimate the prevalence of these arboviral infections in Odisha. About 5198 cases presenting with common clinical symptoms of fever, arthralgia, headache, myalgia and malaise were screened during 2016-2019. A total of 42.2% patients tested positive for dengue NS1 antigen (n = 4154), 30.2% for dengue IgM (n = 2161) and 14.3% for chikungunya IgM (n = 1816). A total of 1684 samples were subjected to Zika RT-PCR and none was tested positive. Peak in the numbers of dengue/ chikungunya cases was evident in the post-monsoon months of July -October. Circulation of all four serotypes of dengue i.e. DEN 1, 2, 3, and 4 was noticed in the state. Molecular investigation of suspected Chik cases in early phases showed circulation of Eastern Central Southern African genotype (E1:226A). There is dearth of knowledge about disease severity during arbovirus co-infections and importance of adequate management of patients at an early stage residing in risk areas. It is the first study in Odisha to study the pattern and status of these three arboviral diseases Dengue, Chikungunya and Zika. The outcome of this study will help in focusing and improvement of existing surveillance systems and vector control tools, as well as on the development of suitable antiviral agents and formulating candidate vaccine.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

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Prevalence and trend of emerging and re-emerging arboviral infections in the state of Odisha

et al. 2021

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“…On an estimate, roughly half of the world population is at the risk of this dengue viral fever (WHO, 2009) [7; 8]. It is interesting to note that, the dengue reported cases is being doubled each year, and about twenty-five thousand people die annually due to this disease [9; 10].…”

Section: Introductionmentioning

confidence: 99%

Assessment of genetic diversity patterns of different serotypes of dengue virus, prevalent in patients from Kerala, India: a strain specific mutation study and its relevance to pathogenesis

Niranjan

Sankari

Manoj

et al. 2023

Preprint

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The mutations are the key mechanisms responsible for virus survival and its fitness in the host. This process of mutation is implicated in the development of pathogenesis of the dengue viral disease. We report that, all the four serotypes were found to be circulating in Kerala sate of India. Serotypes 1 and 3 were the predominant type (33.3 %) compared to others. The prevalence of co-infection of serotype 1 and 4 was the highest percentage (11.1 %) compared to serotype 2 and serotype 3 (5.5 %). We further highlighted the immunopathological mechanisms of antibody neutralization, CD4+ T cell response and antibody dependent enhancements (ADE) for the possible cause of disease severity in coinfections. Serotype-1 does not show much variations from the previously reported strains from various regions of India. However, serotype-2 showed variations in the sequences from the other strains of serotype-2 previously reported from various regions of India and formed a distinct clade in the genotype-4. Serotype-3 and serotype-4 showed similarity with previously reported strains from India. Moreover, serotype-1 was grouping in genotype-5. Importantly, the serotype-2 grouped with genoptype-4 but exist separately. Serotype-3 was found to be grouped with the genotype-3. The serotype-4 show the very much similarities from the genotype-1 and shows little difference from the previously reported strains from India. Further, mutation in DENV-3 sequences, at position 235 (C to T) and 322 (G to T) shows an important phenomenon which might be adopted by the virus to survive. As severe dengue is linked with the serotype-2, the genetic variations in this serotype points towards the much specific strategy to be adopted in near future to manage the severe dengue disease. In conclusion, we can say that, genetic diversity in the CprM region is present in the different serotypes circulating in the patients from Kerala India and this information may help in the management of dengue viral disease.

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“…Natural vertical transmission was the observation of larvae (Du et al, 2021), pupae (Martins et al, 2012), and adult males in infected specimens collected in the field; infected larvae, males, and females raised in the laboratory from eggs collected in the field were also considered (Ferreira-De-Lima and Lima-Camara, 2018). Furthermore, it is vital to reveal the trend of dengue epidemics based on phylogenetic analysis and mutations in the gene sequences of viruses (Sittivicharpinyo et al, 2017;Sabat et al, 2019;Punpapong et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Entomological Investigation and Detection of Dengue Virus Type 1 in Aedes (Stegomyia) albopictus (Skuse) During the 2018–2020 Outbreak in Zhejiang Province, China

Liu

Wang

Hou

et al. 2022

Front. Cell. Infect. Microbiol.

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Mosquito-borne diseases are still threats to public health in the Zhejiang province of China. Surveillance of mosquitoes and the mosquito-borne pathogen is a vital approach for early warning, prevention, and control of the infectious disease. In this study, from 2018 to 2020, a total of 141607 female mosquitoes were caught by means of the light trap method. The main species were Culex pipiens quinquefasciatus/pallens (41.32%), Culex tritaeniorhynchus (47.6%), Aedes albopictus (2.5%), Anopheles sinensis (5.87%), Armigeres subalbatus (2.64%) and other mosquito species (0.07%). Cx. pipiens s.l. were the dominant species in two urban habitats and rural residential areas while Cx. tritaeniorhynchus was the main dominant species in the rural livestock sheds. In terms of seasonal fluctuation, Cx. pipiens s.l fluctuated at a high level from May to October. The peaks of Cx. tritaeniorhynchus, An. sinensis and Ar. subalbatus were in July. In addition, a total of 693 Ae. albopictus were collected with Biogents Mosquitaire CO2 traps in emergency surveillance of dengue fever (DF) and screened for dengue virus infection. There were three circumstances of collection: The first: the sampling time before mosquito control during the local outbreak of DF in Lucheng of Wenzhou, 2019; The second circumstance: the sampling time after mosquito control during the local outbreak of DF of other cities in 2018-2019; The third circumstance: past DF epidemic areas the sampling time before mosquito control during the local outbreak of DF in Lucheng, Wenzhou, Zhejiang, 2019. The pools formed by mosquitoes collected in these three circumstances were 3 (6.1%), 35 (71.5%), and 11 (22.4%) respectively. Of the 49 pools tested, only one in the first circumstance was positive. The full-length dengue virus sequence of ZJWZ/2019 was obtained by sequencing and uploaded to the NCBI as number OK448162. Full-length nucleotide and amino acid homology analyses showed that ZJWZ2019 and Wenzhou DF serum isolates ZJWZ-62/2019 (MW582816) and ZJWZ-18/2019 (MW582815) had the highest homology. The analysis of full genome and E gene phylogenetic trees showed that ZJWZ2019 belonged to serotype 1, genotype I, lineage II, which was evolutionarily related to OK159963/Cambodia/2019. It implies that ZJWZ2019 originated in Cambodia. This study showed the species composition, seasonal dynamics of mosquitoes in different habitats in Zhejiang province and confirmed the role of Ae. albopictus in the transmission cycle of in outbreak of DF in the Lucheng district of Wenzhou in 2019, suggesting the importance of monitoring of vector Aedes infected dengue virus in the prevention and control of DF.

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Molecular and phylogenetic analysis of the dengue strains circulating in Odisha, India

Cited by 6 publications

References 16 publications

Prevalence and trend of emerging and re-emerging arboviral infections in the state of Odisha

Prevalence and trend of emerging and re-emerging arboviral infections in the state of Odisha

Assessment of genetic diversity patterns of different serotypes of dengue virus, prevalent in patients from Kerala, India: a strain specific mutation study and its relevance to pathogenesis

Entomological Investigation and Detection of Dengue Virus Type 1 in Aedes (Stegomyia) albopictus (Skuse) During the 2018–2020 Outbreak in Zhejiang Province, China

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