Molecular and phenotypic identification of Candida isolates from pregnant women in Ogbomoso, Southwestern Nigeria

Nurat, Akinbami Abidemi; Ola, Babalola Gbolahan; Shittu, Mujeeb; Mikhail, Tijani Aramide; Ayodeji, Adekola Saheed

doi:10.18203/2320-1770.ijrcog20160363

Cited by 2 publications

(3 citation statements)

References 11 publications

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“…Chromogenic media are another common group that could be used for both isolation and preliminary identification of some common species. These culture media have the same limitation as corn meal agar and could not differentiate C. dubliniensis and C. africana from C. albicans 47–49 . Similarly, although germ tube test can discriminate between C. albicans and NAC species, it has similar results for C. albicans , C. dubliniensis , and C. africana 49,50 .…”

Section: Diagnosismentioning

confidence: 99%

“…These culture media have the same limitation as corn meal agar and could not differentiate C. dubliniensis and C. africana from C. albicans. [47][48][49] Similarly, although germ tube test can discriminate between C. albicans and NAC species, it has similar results for C. albicans, C. dubliniensis, and C. africana. 49,50 As a solution for these limitations, molecular techniques have been used as complement to conventional methods that provide accurate outputs in a short time (1.5-3 h).…”

Section: Diagnosismentioning

confidence: 99%

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Vulvovaginal candidiasis: An overview of mycological, clinical, and immunological aspects

Jafarzadeh

Ranjbar

Nazari

et al. 2022

J of Obstet and Gynaecol

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Aim: To provide an overview of clinical, immunological, and mycological aspects of vulvovaginal candidiasis (VVC). Methods: A literature search was conducted to find relevant articles about different aspects of VVC. Related data from retrieved articles were summarized in different headings.Results: VVC has a global distribution and Candida albicans is the leading cause of infection except for specific patient groups like postmenopausal, diabetic, or immunocompromised women. VVC has a range of clinical presentations, accordingly, its diagnosis should be based on clinical examination coupled with laboratory investigations. The best therapeutic regimen depends on the patient's conditions and the causative agent. Moreover, factors like drug resistance of the causative agents and different mutations in the immunity-related genes could affect the treatment outcome. Conclusion: As a globally distributed disease, VVC needs further attention, especially in areas related to the treatment failure and recurrence of the disease.

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Section: Diagnosismentioning

confidence: 99%

Section: Diagnosismentioning

confidence: 99%

Vulvovaginal candidiasis: An overview of mycological, clinical, and immunological aspects

Jafarzadeh

Ranjbar

Nazari

et al. 2022

J of Obstet and Gynaecol

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“…Candida species contain pathogens that exist as normal flora of the body of healthy humans, particularly in oral cavity, intestinal tract, and on the skin. They are able to develop a range of diseases including non-life-threatening to life-threatening invasive diseases (5,6).…”

Section: Introductionmentioning

confidence: 99%

Molecular Analysis of Candida species with Emphasis on Predisposing Factors in Cutaneous Candidiasis Patients

Ghasemi

Hashemi

Rezaei

et al. 2016

Jundishapur J Microbiol

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Background: Candida species are known as the most common fungal pathogens isolated from clinical specimens that can develop complications ranging from cutaneous to systemic diseases. Objectives: The present study was conducted to evaluate the diversity and distribution of Candida species in various kinds of cutaneous candidiasis in Tehran, Iran. In addition, the critical effects of several predisposing factors on the induction and progression of the disease were considered. Methods: A total of 3000 samples were taken from March 2014 to 2015. The samples were examined using direct microscopy and culturing method. The polymorphism analysis was performed by using polymerase chain reaction -restriction fragment length polymorphism (PCR-RFLP) technique. The internal spacer region (ITS) of the fungal rRNA genes was recruited for PCR amplification of target sequences and Msp1 enzyme was employed to digest PCR amplicons. Results: Out of 3000 samples, yeast was recovered in 290 (9.67%) cases. Onychomycosis was observed predominantly in 164 nail samples (56.5%). The majority of patients were within the age range of 51 -60 years (22.14%). Women working at home (housewives) showed the highest percentage of people at risk (n = 135, 46.5%). Among patients with underlying diseases, diabetic patients with 46 cases (14.2 %) had the highest susceptibility to candidiasis. Genotypic identification showed that Candida albicans is the most common species (n = 132, 45.5 %) recovered from clinical samples, followed by C. parapsilosis (n = 77, 26.5%), C. glabrata (n = 22, 7.5 %), C. krusei (n = 16, 5.5 %), C. tropicalis (n = 37, 12.7 %), and C. guilliermondii (n = 6, 2%). Conclusions:The results of the present study, compared to the previous studies, showed a significantly lower prevalence of cutaneous candidiasis. Among Candida species, C. albicans was still the most common infectious agent isolated from clinical samples. Shifting toward non-albicans Candida species was not confirmed in this study. In addition, our study revealed that there is a direct correlation between some predisposing factors including age of patients, and existence of some non-infectious diseases (e.g. metabolic disorder) and occurrence of Candida infection.

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Molecular and phenotypic identification of Candida isolates from pregnant women in Ogbomoso, Southwestern Nigeria

Cited by 2 publications

References 11 publications

Vulvovaginal candidiasis: An overview of mycological, clinical, and immunological aspects

Vulvovaginal candidiasis: An overview of mycological, clinical, and immunological aspects

Molecular Analysis of Candida species with Emphasis on Predisposing Factors in Cutaneous Candidiasis Patients

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