Molecular and Phenotypic Characteristics of Salmonella enterica Serovar 4,5,12:i:-Isolated from Cattle and Humans in Iwate Prefecture, Japan

Ido, Noriko; Kudo, Takashi; Sasaki, Koji; Motokawa, Masato; Iwabuchi, Kaori; Matsudate, Hiroki; Seimiya, Yukio; Akiba, Masato

doi:10.1292/jvms.10-0140

Cited by 16 publications

(12 citation statements)

References 14 publications

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“…The monophasic isolates were fully typeable and grouped together with the S. typhimurium isolates, indicating that these isolates most likely originated from S. typhimurium. This view is supported by several studies (Ido et al, 2010;Laorden et al, 2010;Switt et al, 2009;Trupschuch et al, 2010). Our MLVA method displayed 170 (34.8%) of 489 isolates where all five loci were present and typeable.…”

Section: Introductionsupporting

confidence: 81%

Genotyping of selected bacterial enteropathogens in Norway

Lindstedt

2011

International Journal of Medical Microbiology

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Section: Introductionsupporting

confidence: 81%

Genotyping of selected bacterial enteropathogens in Norway

Lindstedt

2011

International Journal of Medical Microbiology

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“…4,[5],12:i:- isolates maintained the genetic structure of the fljAB–hin region, PCR mapping [7] to detect the boundary region of each gene was performed using the S . Typhimurium LT2 DNA sequence as a reference.…”

Section: Resultsmentioning

confidence: 99%

“…The approval from the Institutional Animal Care and Use Committee is not required in case of isolation for diagnostic purpose. Isolates H1–5 and C1–10 were the same as H1–5 and C1–10, respectively, as described in a previous report [7]. Salmonella spp.…”

Section: Methodsmentioning

confidence: 93%

Characteristics of Salmonella enterica Serovar 4,[5],12:i:- as a Monophasic Variant of Serovar Typhimurium

Ido¹,

Lee

Iwabuchi

et al. 2014

PLoS ONE

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Salmonella enterica subspecies enterica serovar 4,[5],12:i:- (S. 4,[5]12:i:-) is believed to be a monophasic variant of S. enterica serovar Typhimurium (S. Typhimurium). This study was conducted to corroborate this hypothesis and to identify the molecular and phenotypic characteristics of the S. 4,[5]12:i:- isolates in Japan. A total of 51 S. 4,[5]12:i:- isolates derived from humans, cattle, swine, chickens, birds, meat (pork), and river water in 15 prefectures in Japan between 2000 and 2010 were analyzed. All the S. 4,[5],12:i:- isolates were identified as S. Typhimurium by two different polymerase chain reactions (PCR) for identification of S. Typhimurium. Of the 51 S. 4,[5],12:i:- isolates, 39 (76.5%) harbored a 94-kb virulence plasmid, which is known to be specific for S. Typhimurium. These data suggest that the S. 4,[5],12:i:- isolates are monophasic variants of S. Typhimurium. The flagellar phase variation is induced by three adjacent genes (fljA, fljB, and hin) in the chromosome. The results of PCR mapping of this region and comparative genomic hybridization analysis suggested that the deletion of the fljAB operon and its flanking region was the major genetic basis of the monophasic phenotype of S. 4,[5],12:i:-. The fljAB operon and hin gene were detectable in eight of the S. 4,[5],12:i:- isolates with common amino acid substitutions of A46T in FljA and R140L in Hin. The introduction of these mutations into S. Typhimurium isolates led to the loss of selectability of isolates expressing the phase 2 H antigen. These data suggested that a point mutation was the genetic basis, at least in part, of the S. 4,[5],12:i:- isolates. The results of phenotypic analysis suggested that the S. 4,[5],12:i:- isolates in Japan consist of multiple distinct clones. This is the first detailed characterization of the S. 4,[5],12:i:- isolates derived from various sources across Japan.

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“…Many of these outbreaks were linked to the consumption of contaminated pork and other sources, such as cattle and poultry (17). Isolation of Salmonella 4, [5],12:i:Ϫ from humans and animals has also become common in Japan in recent years (10,18,19). However, the genetic relatedness between isolates in Japan and Western countries is not clear.…”

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confidence: 99%

Phylogenetic Characterization of Salmonella enterica Serovar Typhimurium and Its Monophasic Variant Isolated from Food Animals in Japan Revealed Replacement of Major Epidemic Clones in the Last 4 Decades

et al. 2018

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serovar Typhimurium ( Typhimurium) and its monophasic variant ( 4,[5],12:i:-) are the major causes of gastroenteritis in both humans and animals. Pulsed-field gel electrophoresis and multilocus variable-number tandem-repeat analysis have been used widely as subtyping methods for these pathogens in molecular epidemiological analyses, but the results do not precisely reflect phylogenetic information. In this study, we performed a phylogenetic analysis of these serovars using whole-genome sequencing data and identified nine distinct genotypic clades. Then, we established an allele-specific PCR-based genotyping method detecting a clade-specific single nucleotide polymorphism to rapidly identify the clade of each isolate. Among a total of 815 isolates obtained from cattle in Japan between 1977 and 2017, clades 1, 7, and 9 contained 77% of isolates. Obvious replacement of the dominant clone was observed five times in this period, and clade 9, which mostly contains 4,[5],12:i:-, is currently dominant. Among 140 isolates obtained from swine in Japan between 1976 and 2017, clades 3 and 9 contained 64% of isolates. Clade 9 is the latest clone as is the case in cattle isolates. Clade 9 is similar to an epidemic clone from Europe, which is characterized by sequence type 34 (ST34), chromosomal genomic island 3, and a composite transposon containing antimicrobial resistance genes. The increased prevalence of clade 9 among food animals in Japan might be a part of the pandemic of the European 4,[5],12:i:- clone.

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Molecular and Phenotypic Characteristics of Salmonella enterica Serovar 4,5,12:i:-Isolated from Cattle and Humans in Iwate Prefecture, Japan

Cited by 16 publications

References 14 publications

Genotyping of selected bacterial enteropathogens in Norway

Genotyping of selected bacterial enteropathogens in Norway

Characteristics of Salmonella enterica Serovar 4,[5],12:i:- as a Monophasic Variant of Serovar Typhimurium

Phylogenetic Characterization of Salmonella enterica Serovar Typhimurium and Its Monophasic Variant Isolated from Food Animals in Japan Revealed Replacement of Major Epidemic Clones in the Last 4 Decades

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