Molecular and pathological characterization of N:O isolates of the<i>Potato virus Y</i>from Manitoba, Canada

Nie, Xianzhou; Singh, R. P.; Singh, Mathuresh

doi:10.1080/07060660409507178

Cited by 70 publications

(49 citation statements)

References 35 publications

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“…N found, giving additional evidence that PVY N strains are a recent introduction into the seed potato system in North America (24 (50,59,60,84) showed that some strains were molecular recombinants, while others were not.…”

Section: Pvymentioning

confidence: 95%

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Potato virus Y: An Evolving Concern for Potato Crops in the United States and Canada

et al. 2010

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Section: Pvymentioning

confidence: 95%

“…(16,59,60,69, and this study) and is problematic because it is not always detected in serological tests used by certification and regulatory agencies as a tobacco necrotic strain. The PVY O -O5 variant accounted for 6 and 3% of the total isolates from the United States and Canada, respectively.…”

Section: Defining the Pvy Problem In The United States And Canadamentioning

confidence: 99%

Potato virus Y: An Evolving Concern for Potato Crops in the United States and Canada

et al. 2010

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“…The necrotic strains were until recently on the list of quarantine pests for the North American Plant Protection Organization (NAPPO) countries (Canada, Mexico, and U.S.) (Anonymous 2003) and detection often resulted in regulatory actions and suspension of cross-border trade. Numerous reports of necrotic strains present in field grown potatoes (Baldauf et al 2006;Crosslin et al 2006;Crosslin et al 2005;Karasev et al 2008;Lorenzen et al 2006a;Nie and Singh 2003;Nie et al 2004;Piche et al 2004;Xu 2008;Xu et al 2005) and the adoption of a US-Canada Binational Tuber Necrotic Virus Management Plan in 2006 has resulted in the removal of necrotic strains of PVY from the quarantine list. Currently the entire PVY complex is managed primarily by seed certification standards.…”

Section: Introductionmentioning

confidence: 97%

Serological Properties of Ordinary and Necrotic Isolates of Potato virus Y: A Case Study of PVYN Misidentification

Karasev

Николаева

et al. 2009

Am. J. Pot Res

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In the course of a multi-year survey of Potato virus Y (PVY) incidence and diversity in the U.S. seed potato crop, an unusual PVY variant was identified in low but significant levels in multiple states. This variant, PVY O -O5, was initially detected by a commercially available PVY N -specific monoclonal antibody, 1F5. This antibody is widely used by U.S. Seed Certification programs to test for PVY N and is one of two antibodies designated by the North American Plant Protection Organization (NAPPO) for preshipment testing of tuber lots that are to be transported between countries. Consequently, PVY N positives identified by the 1F5 antibody have triggered quarantine actions, prevented cross-border shipments and impacted trade. Here, we demonstrate by a variety of methods that the PVY O -O5 is a variant within the ordinary PVY strain (PVY O ). Specifically, the PVY O -O5 variant likely arose due to a single amino acid substitution within the capsid protein. This variant does not induce vein necrosis in tobacco or tuber necrosis in susceptible varieties of potato. Furthermore, it is identified by RT-PCR based diagnostics as PVY O and it has a typical PVY O genome sequence. We demonstrate that another PVY N specific monoclonal antibody, SASA-N, recognizes an epitope distinct from that recognized by 1F5, and correctly identifies the PVY O -O5 variants as belonging to the PVY O serotype. Since the PVY O -O5 variant is present in many seed producing states and misidentification of PVY O -O5 as PVY N/NTN has clear quarantine implications for export shipments of potato, the limitations of the commercially available monoclonal antibodies should be considered in any certification or phytosanitary testing program.Resumen A lo largo del estudio de varios años sobre la incidencia y diversidad del virus Y de la papa (PVY) en los cultivos de papa para semilla en los Estados Unidos (EU), se identificó a una variante inusual a niveles bajos pero significativos en múltiples estados. Esta variante, PVY O -O5, se detectó inicialmente con un anticuerpo monoclonal comercialmente disponible específico para PVY N , el 1F5. Este anticuerpo es ampliamente usado por los Programas de Certificación de Semilla en los EU para PVY N , y es uno de los dos anticuerpos designados por la Organización Norteamericana de Protección de Plantas (NAPPO) para pruebas de pre-envío de lotes de tubérculos que serán transportados entre países. Consecuentemente, los PVY N positivos identificados con el anticuerpo 1F5 han disparado acciones cuarentenarias, evitando envíos trans-fronteras y han impactado al comercio. Aquí, nosotros demostramos con diversos métodos que PVY O -O5 es una variante del PVY ordinario (PVY O ). Específicamente, la variante PVY O -O5 es probable que haya surgido debido a una substitución de un aminoácido dentro de la proteína de la cápside. Esta variante no induce necrosis de las venas en tabaco o necrosis del A. V. Karasev : O. V. Nikolaeva : X. Hu : Z. Sielaff : tubérculo en variedades susceptibles de papa. Aún mas, se le identifica c...

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“…Potato virus Y (PVY) is an economically important pathogen of potato worldwide causing significant losses to both yield and quality (Gray et al, 2010;Karasev and Gray, 2013;Nie et al, 2004). PVY belongs to the family Potyviridae, and genus Potyvirus.…”

Section: Introductionmentioning

confidence: 99%

Comparative analysis of virus-specific small RNA profiles of three biologically distinct strains of Potato virus Y in infected potato (Solanum tuberosum) cv. Russet Burbank

Naveed¹,

Mitter²,

Harper³

et al. 2014

Virus Research

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Molecular and pathological characterization of N:O isolates of thePotato virus Yfrom Manitoba, Canada

Cited by 70 publications

References 35 publications

Potato virus Y: An Evolving Concern for Potato Crops in the United States and Canada

Potato virus Y: An Evolving Concern for Potato Crops in the United States and Canada

Serological Properties of Ordinary and Necrotic Isolates of Potato virus Y: A Case Study of PVYN Misidentification

Comparative analysis of virus-specific small RNA profiles of three biologically distinct strains of Potato virus Y in infected potato (Solanum tuberosum) cv. Russet Burbank

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