Molecular and Pathogenic Diversity Among Brazilian Isolates of Xanthomonas albilineans Assessed with SSR Marker Loci

Tardiani, Ana C.; Pérecin, Dilermando; Peixoto-Junior, Rafael Fávero; Sanguino, A; Landell, Marcos Guimarães de Andrade; Beriam, L. O. S.; Nunes, Daniel S.; Camargo, L. E. A.; Creste, Silvana

doi:10.1094/pdis-07-13-0762-re

Cited by 14 publications

(30 citation statements)

References 37 publications

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“…Fifteen SSR markers developed by Tardiani et al (2014) were used to assess the diversity and population structure of the 40 X. albilineans strains from China. The PCR reaction was performed in a volume of 20 µL consisting of 1x HotStarTaq buffer, 2.0 mM Mg 2+ , 0.2 mM dNTP, 0.2 U HotStarTaq polymerase (Qiagen Inc, Frankfurt, Germany), 1.0 µM each of the forward and reverse primers, and 10-30 ng/µl of genomic DNA.…”

Section: Ssr Genotyping Through Sequencingmentioning

confidence: 99%

Sugarcane responses to two strains of Xanthomonas albilineans differing in pathogenicity through a differential modulation of salicylic acid and reactive oxygen species

et al. 2022

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Leaf scald caused by Xanthomonas albilineans is one of the major bacterial diseases of sugarcane that threaten the sugar industry worldwide. Pathogenic divergence among strains of X. albilineans and interactions with the sugarcane host remain largely unexplored. In this study, 40 strains of X. albilineans from China were distributed into three distinct evolutionary groups based on multilocus sequence analysis and simple sequence repeats loci markers. In pathogenicity assays, the 40 strains of X. albilineans from China were divided into three pathogenicity groups (low, medium, and high). Twenty-four hours post inoculation (hpi) of leaf scald susceptible variety GT58, leaf populations of X. albilineans strain XaCN51 (high pathogenicity group) determined by qPCR were 3-fold higher than those of strain XaCN24 (low pathogenicity group). Inoculated sugarcane plants modulated the reactive oxygen species (ROS) homoeostasis by enhancing respiratory burst oxidase homolog (ScRBOH) expression and superoxide dismutase (SOD) activity and by decreasing catalase (CAT) activity, especially after infection by X. albilineans XaCN51. Furthermore, at 24 hpi, plants infected with XaCN51 maintained a lower content of endogenous salicylic acid (SA) and a lower expression level of SA-mediated genes (ScNPR3, ScTGA4, ScPR1, and ScPR5) as compared to plants infected with XaCN24. Altogether, these data revealed that the ROS production-scavenging system and activation of the SA pathway were involved in the sugarcane defense response to an attack by X. albilineans.

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Section: Ssr Genotyping Through Sequencingmentioning

confidence: 99%

Sugarcane responses to two strains of Xanthomonas albilineans differing in pathogenicity through a differential modulation of salicylic acid and reactive oxygen species

et al. 2022

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“…Microsatellite typing allowed gaining knowledge on the population biology of several xanthomonads. More specifically, it was shown useful for discriminating between genetically-related populations differing in pathogenicity [24], deciphering the geographical structure of Xanthomonas populations at scales ranging from a single field to a production basin and placing hypotheses on source populations [26,192,193,246,264,[267][268][269][270], testing approximate Bayesian computation-based invasion scenarios [236], emphasizing the importance of plant propagative material as the source of disease emergence [236,265,271,272], estimating the range of spatial dependency of outbreaks and assessing the biological significance of coinfections in single lesions [266].…”

Section: Markers For Population/epidemiology Studies 421 Multilocus Variable Number Of Tandem Repeats Analysismentioning

confidence: 99%

Trends in Molecular Diagnosis and Diversity Studies for Phytosanitary Regulated Xanthomonas

et al. 2021

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Bacteria in the genus Xanthomonas infect a wide range of crops and wild plants, with most species responsible for plant diseases that have a global economic and environmental impact on the seed, plant, and food trade. Infections by Xanthomonas spp. cause a wide variety of non-specific symptoms, making their identification difficult. The coexistence of phylogenetically close strains, but drastically different in their phenotype, poses an added challenge to diagnosis. Data on future climate change scenarios predict an increase in the severity of epidemics and a geographical expansion of pathogens, increasing pressure on plant health services. In this context, the effectiveness of integrated disease management strategies strongly depends on the availability of rapid, sensitive, and specific diagnostic methods. The accumulation of genomic information in recent years has facilitated the identification of new DNA markers, a cornerstone for the development of more sensitive and specific methods. Nevertheless, the challenges that the taxonomic complexity of this genus represents in terms of diagnosis together with the fact that within the same bacterial species, groups of strains may interact with distinct host species demonstrate that there is still a long way to go. In this review, we describe and discuss the current molecular-based methods for the diagnosis and detection of regulated Xanthomonas, taxonomic and diversity studies in Xanthomonas and genomic approaches for molecular diagnosis.

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“…Plants were grown in individual pots containing a mixture of soil, sand, pine, and coconut bark substrate at 3:1:1:1 proportion. Twenty-five sugarcane stalks per cultivar were inoculated by the decapitation method using X. albilineans strain IACXa11, considered one of the most aggressive X. albilineans isolates found in Brazil (Tardiani et al, 2014), as described by Rott et al, (2011) with modifications. Stalks were cut off using sterile scissors just above leaf+2, and inoculated using 100 µL of bacterial suspension calibrated to 1x10 8 CFU mL -1 in sterile PBS buffer.…”

Section: Biological Materialsmentioning

confidence: 99%

Research Article Early down-regulation of chorismate mutase 2 gene expression in a sugarcane cultivar tolerant to Xanthomonas albilineans infection

Rosa-Santos¹,

Nogueira²,

Paz³

et al. 2019

Genet. Mol. Res.

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Xanthomonas albilineans causes leaf scald disease, one of the most economically important diseases of sugarcane. Leaf scald is a systemic and vascular disease that can severely reduce productivity. The best and primary method of leaf scald control is the use of tolerant cultivars. Knowing that cell wall lignification is a plant defense mechanism and that chorismate mutase is relevant in this process, we analyzed the expression pattern of the chorismate mutase 2 (SoCM2) gene in two contrasting sugarcane cultivars by RT-qPCR. A similar pattern of downregulation for the SoCM2 gene was observed in both cultivars; however, after 72h, the tolerant cultivar (RB867515) showed repression of the SoCM2 gene, while for the susceptible cultivar (SP78-4467), SoCM2 gene downregulation occurred only after 144h. Here we propose that early T.M. repression of the SoCM2 gene is responsible for tolerance to X. albilineans infection in the sugarcane cultivar RB867515.

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Molecular and Pathogenic Diversity Among Brazilian Isolates of Xanthomonas albilineans Assessed with SSR Marker Loci

Cited by 14 publications

References 37 publications

Sugarcane responses to two strains of Xanthomonas albilineans differing in pathogenicity through a differential modulation of salicylic acid and reactive oxygen species

Sugarcane responses to two strains of Xanthomonas albilineans differing in pathogenicity through a differential modulation of salicylic acid and reactive oxygen species

Trends in Molecular Diagnosis and Diversity Studies for Phytosanitary Regulated Xanthomonas

Research Article Early down-regulation of chorismate mutase 2 gene expression in a sugarcane cultivar tolerant to <i>Xanthomonas</i> <i>albilineans</i> infection

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