Molecular and nanoparticle postcolumn reagents for assay of low-molecular-mass biothiols using high-performance liquid chromatography

Nanomaterials as tools in chromatographic methods

“…Metal NPs have been widely described as post-column derivatizing reagents using: 1) their aggregation induced by the analytes and measuring absorbance or resonance light scattering [57,58], and 2) their catalytic effect in different chemiluminescent systems, which is modified in the presence of the analytes [59,60].…”

“…The aggregation of AuNPs by thiol-containing analytes is a relatively slow process but it can be accelerated by using suitable capping ligands for AuNPs [57,58].…”

Colorimetric determination of biothiols with AuNPs@MoS2 NSs as a peroxidase mimetic enzyme

“…Thus far, several biothiol determination methods have been developed, for example, capillary electrophoresis, 7,8 mass spectrometry (MS), 9,10 and high-performance liquid chromatography (HPLC). 11,12 However, expensive instruments, high professional requirements, and low efficiency limit the application of these methods in medical routine tests. 13,14 By contrast, the colorimetric method is considered a promising biothiol detection strategy because of its simplicity, inexpensiveness, efficiency, and ability to be used in direct visual readout.…”

Polyinosinic acid-stabilized fluorescent silver nanoclusters for sensitive detection of biological thiols

“…Low-molecular mass biothiols such as cysteine (Cys), homocysteine (Hcy), and glutathione (GSH) have been proved to cause serious diseases, 1,2 and hence it is signicant to develop a simple and convenient method for quantitative assay of these thiols in biological uids. Therefore, a large number of approaches have been developed, including high-performance liquid chromatography, 3,4 electrochemistry, [5][6][7] and mass spectroscopy. 8 However, some expensive and sophisticated instruments were usually involved, as well as the complicated analytical procedures.…”