Molecular and morphometric analyses reveal cryptic diversity within freshwater mussels (Bivalvia: Unionidae) of the western Gulf coastal drainages of the USA

Pieri, Anna M; Inoue, Kentaro; Johnson, Nathan A.; Smith, Chase H.; Harris, John L.; Robertson, Clint; Randklev, Charles R.

doi:10.1093/biolinnean/bly046

Cited by 31 publications

(55 citation statements)

References 54 publications

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“…Our study represents the first robust phylogenetic evaluation of Leptodea and Potamilus with comprehensive taxon sampling and evaluation of both mtDNA and nDNA loci. Despite employing multiple independently evolving markers used in recent freshwater mussel phylogenetic studies Lopes-Lima et al, 2017;Perkins et al, 2017;Pfeiffer, Sharpe, Johnson, Emery, & Page, 2018;Pfeiffer et al, 2016;Pieri et al, 2018;Smith et al, 2018), we could not resolve topologies that strongly support phylogenetic relationships between Leptodea and Potamilus. Therefore, we take a precautionary approach by not making any conclusions regarding generic-level assignments at this time.…”

Section: Supraspecific Relationships In Lampsilinimentioning

confidence: 75%

“…nov. from all populations of P. amphichaenus. Genetic distances between the two entities are similar to or greater than patterns of specieslevel diversity in other unionids (Inoue et al, 2014a;Jones, Neves, Ahlstedt, & Hallerman, 2006;Pfeiffer et al, 2016;Pieri et al, 2018;Roe & Lydeard, 1998), and haplotype networks depicting clear molecular separation between P. streckersoni sp. nov. and P. amphichaenus with no haplotype sharing at either mtDNA markers (Figs 3.1 & 3.2).…”

Section: Species Boundaries In the Potamilus Ohiensis Species Complexmentioning

confidence: 80%

“…PCR products were sent to the Molecular Cloning Laboratories (MCLAB, South San Francisco, CA, USA) for bi-directional sequencing on an ABI 3730. All ITS1 sequences were readable without cloning, similar to recent studies in unionids Pfeiffer et al, 2016;Pieri et al, 2018;Smith et al, 2018). Geneious v 10.2.3 was used to assemble contigs and edit chromatograms (Kearse et al, 2012) and sequences were aligned in Mesquite v 3.31 (Maddison & Maddison, 2017) using MAFFT v 7.311 (Katoh & Standley, 2013).…”

Section: Taxon Sampling and Molecular Data Generationmentioning

confidence: 99%

“…However, this hypothesis has not been validated using molecular techniques. The possibility of a syntopic form of P. ohiensis with P. amphichaenus is troubling, especially considering P. amphichaenus is petitioned for listing under the Endangered Species Act (USFWS, 2009) and a recent phylogenetic study revealed multiple morphologically cryptic sympatric species of Fusconaia Simpson, 1900 in the Trinity River (Pieri et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Integrative taxonomy reveals a new species of freshwater mussel, Potamilus streckersoni sp. nov. (Bivalvia: Unionidae): implications for conservation and management

Smith

Johnson

Inoue³

et al. 2019

Systematics and Biodiversity

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Inaccurate systematics confound our ability to determine evolutionary processes that have led to the diversification of many taxa. The North American freshwater mussel tribe Lampsilini is one of the better-studied groups in Unionidae, however, many supraspecific relationships between lampsiline genera remain unresolved. Two genera previously hypothesized to be non-monophyletic that have been largely overlooked are Leptodea and Potamilus. We set out to resolve supraspecific relationships in Lampsilini and test the monophyly of Leptodea and Potamilus by integrating molecular, morphological, and life history data. Our molecular matrix consisted of four loci: cytochrome c oxidase subunit 1 (CO1), NADH dehydrogenase subunit 1 (ND1), internal transcribed spacer 1 (ITS1), and 28S ribosomal RNA. Secondly, we performed both traditional and Fourier shape morphometric analyses to evaluate morphological differences and finally, we compared our results with available life history data. Molecular data supported the paraphyly of both Leptodea and Potamilus, but nodal support was insufficient to make any conclusions regarding generic-level assignments at this time. In contrast, inference from our integrative taxonomic assessment depicts significant support for the recognition of a new species, Potamilus streckersoni sp. nov., the Brazos Heelsplitter. Our data show clear separation of three taxonomic entities in the P. ohiensis species complex: P. amphichaenus, P. ohiensis, and P. streckersoni sp. nov.; all molecularly, geographically, and morphologically diagnosable. Our findings have profound implications for unionid taxonomy and will aid stakeholders in establishing effective conservation and management strategies. http://www.zoobank.org/urn:lsid:zoobank.org:pub:502647C0-418B-4CC4-85A8-BD89FC3F674F

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Section: Supraspecific Relationships In Lampsilinimentioning

confidence: 75%

Section: Species Boundaries In the Potamilus Ohiensis Species Complexmentioning

confidence: 80%

Section: Taxon Sampling and Molecular Data Generationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Integrative taxonomy reveals a new species of freshwater mussel, Potamilus streckersoni sp. nov. (Bivalvia: Unionidae): implications for conservation and management

Smith

Johnson

Inoue³

et al. 2019

Systematics and Biodiversity

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“…(either Fusconaia flava [Wabash Pigtoe], or Fusconaia chunii [Trinity Pigtoe]; see Pieri et al. for details regarding why these two species cannot be differentiated in the field), Tritogonia verrucosa (Pistolgrip), Tritogonia nobilis (Gulf Mapleleaf), Megalonaias nervosa (Washboard), and T. macrodon . Of these, three ( L. fragilis , P. purpuratus , and T. macrodon ) had purity and reliability values that exceeded quality control criteria, which could be the result of small sample sizes or nonresponse to RSS.…”

Section: Resultsmentioning

confidence: 99%

Hydraulic requirements of freshwater mussels (Unionidae) and a conceptual framework for how they respond to high flows

Randklev¹,

Hart²,

Khan³

et al. 2019

Ecosphere

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Spatiotemporal variability in flow determines the physical structures of habitat. During low flows, aquatic organisms can be exposed to reduced dissolved oxygen concentrations, increased water temperature, and desiccation, whereas at high flows, increased velocity and hydraulic forces on the streambed can be equally detrimental. These constraints create a mosaic of habitat that influences the distribution and abundance of aquatic biota. This mosaic can change due to stochastic events or those mediated by humans. Understanding how low and high flow conditions affect aquatic organisms is critical not only for advancing ecological knowledge but also for protecting imperiled aquatic species such as unionid mussels. The overall goal of this project was to examine how substrate and hydrologic conditions affect mussel habitat and to then use the resulting information combined with life‐history traits and shell morphology (i.e., sculpturing) to better understand how flow shapes mussel assemblage structure. Using quantile regression, we found that low values of relative shear stress (RSS), a measure of substrate stability, were associated with high mussel species richness and density. Change point analysis using threshold indicator taxa analysis (TITAN) indicated species‐specific preferences for varying levels of bed stability. These preferences were best explained by life‐history strategy and shell morphology based on the results of a principal component analysis. Using these results, we then present a conceptual model from which to derive expectations concerning taxonomic composition, life‐history strategy, and shell sculpture type based on the degree of substrate mobility using RSS and variability in RSS.

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Editorial: The importance of integrated taxonomy to freshwater conservation

Gangloff

2019

Aquatic Conservation

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Molecular and morphometric analyses reveal cryptic diversity within freshwater mussels (Bivalvia: Unionidae) of the western Gulf coastal drainages of the USA

Cited by 31 publications

References 54 publications

Integrative taxonomy reveals a new species of freshwater mussel, Potamilus streckersoni sp. nov. (Bivalvia: Unionidae): implications for conservation and management

Integrative taxonomy reveals a new species of freshwater mussel, Potamilus streckersoni sp. nov. (Bivalvia: Unionidae): implications for conservation and management

Hydraulic requirements of freshwater mussels (Unionidae) and a conceptual framework for how they respond to high flows

Editorial: The importance of integrated taxonomy to freshwater conservation

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