Molecular and Morphological Delimitation of Species in the Group of Lepocinclis Ovum ‐like taxa (Euglenida)

Fells

et al. 2021

Journal of Phycology

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Environmental sampling in Poland and the United States and phylogenetic analyses based on 567 sequences of four genes (155 sequences of nuclear SSU rDNA, 139 of nuclear LSU rDNA, 135 of plastid‐encoded SSU rDNA, and 138 of plastid‐encoded LSU rDNA) resulted in description of the new genus Flexiglena, which has been erected by accommodating Euglena variabilis, and enriching the Discoplastis and Euglenaformis genera with five new species. Four of them have joined the Discoplastis genus, currently consisting of six representatives: D. adunca, D. angusta (=Euglena angusta), D. constricta (=Lepocinclis constricta), D. excavata (=E. excavata), D. gasterosteus (=E. gasterosteus), and D. spathirhyncha. One of them has enriched the Euglenaformis genus, currently represented by two species: Euf. chlorophoenicea (= E. chlorophoenicea) and Euf. proxima. For most studied species, the diagnostic descriptions have been emended and epitypes were designated. Furthermore, the emending of Discoplastis and Euglenaformis diagnoses was performed.

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confidence: 99%

Description of Flexiglena gen. nov. and new members of Discoplastis and Euglenaformis (Euglenida)

Fells

et al. 2021

Journal of Phycology

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“…This occurs in several common euglenid species with very high intraspecific genetic diversity, e.g., Lepocinclis fusiformis up to 7.8%, L. hispidulus up to 5.1% (Łukomska‐Kowalczyk et al. 2020), and P. circumflexus up to 4.9% (Łukomska‐Kowalczyk et al. 2015).…”

Section: Discussionmentioning

confidence: 99%

“…To obtain some sequences, the nested PCR method was used as described previously (Łukomska‐Kowalczyk et al. 2020).…”

Section: Methodsmentioning

confidence: 99%

“…Recently, Phacus horridus (= P. hispidulus from the Acanthochloris section) was reclassified as a member of Lepocinclis (Bennett and Triemer 2012, Łukomska‐Kowalczyk et al. 2020). As a result of all previous morphological‐molecular studies, descriptions were emended, epitypes were designated, many species were renamed, and nine new taxa were described (Kim et al.…”

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confidence: 99%

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Taxon‐rich phylogeny and taxonomy of the genusPhacus(Euglenida) based on morphological and molecular data

Fells

et al. 2020

Journal of Phycology

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Morphological and molecular features were analyzed for a species of Phacus to better understand the phylogenetic relationships among them and establish the taxonomy. Phylogenetic analyses were based on nSSU rDNA and the research resulted in 55 new sequences. The study included species available in algal collections and those isolated directly from the environment in Poland and the Czech Republic. As a result, the obtained phylogenetic tree of Phacus includes 50 species, out of which 7 are represented on a tree for the first time (Phacus anacoelus, P. anomalus, P. curvicauda, P. elegans, P. lismorensis, P. minutus and P. stokesii) and many have been taxonomically verified. For all verified species, diagnostic descriptions were amended, the naming was reordered and epitypes were designated.

“…In previous reports, Chelex (CHEL) was used for DNA extraction from various organisms, including algae (Hamilton et al, 2015;Lienhard and Schäffer, 2019). Herein, a 10% (wt./vol.)…”

Section: Extraction With Chelating Resinmentioning

confidence: 99%

Evaluation of V2 18S rDNA barcode marker and assessment of sample collection and DNA extraction methods for metabarcoding of autotrophic euglenids

Gumińska

Environmental Microbiology

et al. 2021

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Even though the interest in metabarcoding in environmental studies is growing, euglenids are still underrepresented in both sea and freshwater bodies researches. The reason for this situation could be the unsuitability of universal eukaryotic DNA barcodes and primers as well as the lack of a verified protocol, suitable to assess euglenid diversity. In this study, using specific primers for the V2 hypervariable region of 18S rDNA for metabarcoding resulted in obtaining a high fraction (85%) of euglenid reads and species-level identification of almost 90% of them. Fifty species were detected by the metabarcoding method, including almost all species observed using a light microscope. We investigated three biomass harvesting methods (filtering, centrifugation and scraping the side of a collection vessel) and determined that centrifugation and filtration outperformed scrapes, but the choice between them is not crucial for the reliability of the analysis. In addition, eight DNA extraction methods were evaluated. We compared five commercially available DNA isolation kits, two CTAB-based protocols and a chelating resin. For this purpose, the efficiency of extraction, quality of obtained DNA, preparation time and generated costs were taken into consideration. After examination of the aforementioned criteria, we chose the GeneMATRIX Soil DNA Purification Kit as the most suitable for DNA isolation.