Molecular and morphological clocks for estimating evolutionary divergence times

Abstract

Background: Matrices of morphological characters are frequently used for dating species divergence times in systematics. In some studies, morphological and molecular character data from living taxa are combined, whereas others use morphological characters from extinct taxa as well. We investigated whether morphological data produce time estimates that are concordant with molecular data. If true, it will justify the use of morphological characters alongside molecular data in divergence time inference. Results: We systematically analyzed three empirical datasets from different species groups to test the concordance of dates of species divergence inferred using molecular and discrete morphological data from extant taxa as test cases. We found a high correlation between their divergence time estimates, despite a poor linear relationship between branch lengths for morphological and molecular data mapped onto the same phylogeny. This was because node-to-tip distances showed a much higher correlation than branch lengths, because of an averaging effect over multiple branches. We found that nodes with a large number of taxa often benefit from such averaging, but some considerable discordance between time estimates from molecules and morphology may still occur because some deeper branches show large difference from two types of data. Conclusions: Our findings suggest that node- and tip-calibration approaches may be better suited for nodes with a large number of taxa. Nevertheless, we highlight the importance of evaluating the concordance of time structure in morphological and molecular data before any dating analysis using combined datasets.

Molecular and morphological clocks for estimating evolutionary divergence times

“…The datasets generated and/or analyzed during the current study are available in the figshare repository: https://doi.org/10.6084/m9.figshare.9775730. [48] Ethics approval and consent to participate Not applicable.…”

Molecular and morphological clocks for estimating evolutionary divergence times

“…The datasets generated and/or analyzed during the current study are available in the figshare repository: https://doi.org/10.6084/m9.figshare.9775730. [46] Ethics approval and consent to participate Not applicable.…”