Molecular and morphological characterization of the amaryllis lesion nematode, <i>Pratylenchus hippeastri</i> (Inserra et al., 2007), from California

Handoo, Zafar A.; Skantar, Andrea M.; Kantor, Mihail; Hafez, S. L.; Hult, Maria N.

doi:10.21307/jofnem-2020-058

Cited by 6 publications

(3 citation statements)

References 15 publications

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“…The genus Pratylenchus Filipjev, 1936, is one of the most important nematode genera in terms of the economic impact they have on crops [ 1 , 2 ]. Currently, the genus contains approximately 100 species [ 3 , 4 , 5 ], with new species being described very frequently. The root-lesion nematodes are ranked as the third most important group of plant-parasitic nematodes after root-knot and cyst nematodes [ 2 ] in terms of economic loss in agriculture and horticulture.…”

Section: Introductionmentioning

confidence: 99%

Morphological and Molecular Characterization of Pratylenchus dakotaensis n. sp. (Nematoda: Pratylenchidae), a New Root-Lesion Nematode Species on Soybean in North Dakota, USA

Handoo

Yan

Kantor

et al. 2021

Plants

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Root-lesion nematodes (Pratylenchus spp.) of the genus Pratylenchus Filipjev, 1936, are among the most important nematode pests on soybean (Glycine max (L.) Merr.), along with soybean cyst and root-knot nematodes. In May 2015 and 2016, a total of six soil samples were collected from a soybean field in Walcott, Richland County, ND and submitted to the Mycology and Nematology Genetic Diversity and Biology Laboratory (MNGDBL), USDA, ARS, MD for analysis. Later, in 2019, additional nematodes recovered from a greenhouse culture on soybean originally from the same field were submitted for further analysis. Males, females, and juveniles of Pratylenchus sp. were recovered from soil and root samples and were examined morphologically and molecularly. DNA from single nematodes were extracted, and the nucleotides feature of three genomic regions targeting on the D2–D3 region of 28S rDNA and ITS rDNA and mitochondrial cytochrome oxidase subunit I (COX1) gene were characterized. Phylogeny trees were constructed to ascertain the relationships with other Pratylenchus spp., and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was performed to provide a rapid and reliable differentiation from other common Pratylenchus spp. Molecular features indicated that it is a new, unnamed Pratylenchus sp. that is different from morphologically closely related Pratylenchus spp., including P. convallariae, P. pratensis, P. fallax, and P. flakkensis. In conclusion, both morphological and molecular observations indicate that the North Dakota isolate on soybean represents a new root-lesion nematode species which is named and described herein as Pratylenchus dakotaensis n. sp.

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Section: Introductionmentioning

confidence: 99%

Morphological and Molecular Characterization of Pratylenchus dakotaensis n. sp. (Nematoda: Pratylenchidae), a New Root-Lesion Nematode Species on Soybean in North Dakota, USA

Handoo

Yan

Kantor

et al. 2021

Plants

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“…The P. scribneri sequences were aligned with other Pratylenchus species published in GenBank using the nucleotide BLAST program in NCBI (). Nacobbus aberrans , Tylenchorhynchus dubius and Meloidogyne incognita were chosen as the outgroup taxa according to the results published by Nguyen et al (2017) and Handoo et al (2020). Multiple alignments of the sequences were performed using Clustal W in MEGA 7 (Tamura et al , 2011).…”

Section: Methodsmentioning

confidence: 99%

Identification and pathogenicity ofPratylenchus scribnerion tomato in Sichuan Province of People's Republic of China

Xia

Sun

et al. 2022

J. Helminthol.

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Root-lesion nematodes (Pratylenchus spp.) are a group of economically important pathogens that have caused serious economic losses in many crops. In 2019, root-lesion nematodes were recovered from tomato (Solanum lycopersicum) root samples collected from Sichuan Province, People's Republic of China (PRC). Extracted nematodes were disinfected, and one individual female was cultured on a carrot disc for propagation at 25 °C by parthenogenesis and designated the SC isolate. Afterwards, the isolate was identified on the basis of morphometric and molecular markers. Both morphometric characters and molecular analysis of the internal transcribed spacer region gene (ITS) of ribosomal DNA, the D2-D3 expansion region of the 28S rDNA gene and the mitochondrial cytochrome oxidase I (mtDNA-COI) gene revealed that the species of root-lesion nematode was Pratylenchus scribneri. The Bayesian tree inferred from the ITS rDNA, 28S rDNA and mtDNA-COI gene sequences also showed that this isolate formed a highly supported clade with other P. scribneri isolates. The pathogenicity of the root-lesion nematode SC isolate on tomato was assessed, showing that tomato was a suitable host for P. scribneri. To the best of our knowledge, this is the first report of P. scribneri on tomato in Sichuan Province, PRC. These are also the first molecular data obtained from P. scribneri on tomato in the PRC, and the pathogenicity of P. scribneri to tomato was studied for the first time. This study provides scientific data for the detection, identification and control of tomato root-lesion nematode disease.

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“…No entanto, não se aplica a outros gêneros de fitonematoides e apenas pode ser feito a partir de fêmeas maduras. Vários métodos moleculares foram desenvolvidos nas últimas décadas para identificação de fitonematoides, principalmente baseados em reação em cadeia da polimerase (Devran & Söğüt, 2009;Araya et al, 2016;Janssen et al, 2017;Fanelli et al, 2017;Handoo et al, 2020).…”

Section: Municípiounclassified

Levantamento populacional do nematoide das galhas em cafeeiros do Cerrado mineiro e diagnose de Meloidogyne exigua por meio de amplificação isotérmica mediada por loop (LAMP)

Babilônia¹

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O nematoide das galhas (Meloidogyne spp.) parasita as principais culturas agricolas, incluindo o cafeeiro. O ataque de nematoide das galhas ao cafeeiro causa prejuizos à cadeia produtiva do café e para o controle eficiente deste fitopatógeno é importante ressaltar algumas medidas, como o levantamento populacional por regiões, afim de evitar a introdução do patógeno na lavoura, e a correta identificação da espécie. Visando tais medidas, abordou-se neste trabalho o levantamento populacional do nematoide das galhas na região produtora de café do Cerrado mineiro, e o estabelecimento de um ensaio para a utilização da amplificação isotérmica mediada por loop (LAMP) a fim de identificar M. exigua. No Capítulo 1, abordou-se levantamento populacional do nematoide das galhas no Cerrado mineiro. Após a coleta, as amostras foram identificadas utilizando eletroforese de isoenzimas, reação em cadeia da polimerase, observação dos padrões perineais da fêmea e da morfologia do macho. Após o levantamento, a espécie mais encontrada foi M. exigua, seguida de M. paranaensis e M. incognita. Também foi diagnosticado em uma amostra M. arenaria. No Capítulo 2, foi realizada uma revisão sobre a utilização da LAMP na identificação de fitonematoides, discutindo detalhes da técnica e seu potencial para diagnose desses patógenos. No Capítulo 3 estabelecemos um ensaio LAMP para a identificação de M. exigua, com primers baseados na região 28S do rDNA do patógeno, sendo monitorada em gel de eletroforese e a olho nu por colorimetria, utilizando o mastermix WarmStant?. A reação proposta foi capaz de amplificar especificamente DNA de M. exigua, não amplificando de M. incognita e M. paranaensis. Além disso, o ensaio LAMP foi altamente sensível, amplificando 0,0026 ng de DNA alvo. Palavras-chave: Identificação. Isoenzimas. Medidas. Patógeno.

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Molecular and morphological characterization of the amaryllis lesion nematode, Pratylenchus hippeastri (Inserra et al., 2007), from California

Abstract: Root-lesion nematodes (Pratylenchus spp.) are among the most important nematode pests on grapevine along with root-knot, dagger, and ring nematodes. In 2019, two samples of both soil and roots were collected from a vineyard in Delano, Kern County, California and submitted to the

Cited by 6 publications

References 15 publications

Morphological and Molecular Characterization of Pratylenchus dakotaensis n. sp. (Nematoda: Pratylenchidae), a New Root-Lesion Nematode Species on Soybean in North Dakota, USA

Morphological and Molecular Characterization of Pratylenchus dakotaensis n. sp. (Nematoda: Pratylenchidae), a New Root-Lesion Nematode Species on Soybean in North Dakota, USA

Identification and pathogenicity ofPratylenchus scribnerion tomato in Sichuan Province of People's Republic of China

Levantamento populacional do nematoide das galhas em cafeeiros do Cerrado mineiro e diagnose de Meloidogyne exigua por meio de amplificação isotérmica mediada por loop (LAMP)

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