Molecular and morphological characterization of Colletotrichum gloeosporioides from native Mexican Stylosanthes species

Abstract

A total of 264 Stylosanthes spp. plants collected from 78 Stylosanthes spp. populations in seven southern Mexican states were analysed for the presence of Colletotrichum spp. Isolates were obtained from 64 plants collected from 36 Stylosanthes populations; 198 isolates produced straight conidia, while 72 isolates produced falcate conidia. Molecular identification was performed to confirm the identity of C. gloeosporioides for the straight-spored isolates. PCR amplifications using the primer CgInt, synthesized from an ITS1 fragment specific to C. gloeosporioides, and the universal primer ITS4 generated the target fragment for 120 Mexican isolates with straight conidia. The endonucleases Ava II and Sma I were used for restriction of the entire amplified ITS1 region of these 120 isolates. The tree constructed from the restriction data grouped 118 Mexican C. gloeosporioides isolates into three clusters containing reference isolates from Africa and Australia, and generated two additional clusters for two Mexican isolates. Conidial shape and growth rate on solid medium were used as the major morphological criteria for distinguishing types A and B. On the basis of 32 other morphological characteristics, a phenogram grouped the colonies into three main clusters. These clusters were partially related to the Stylosanthes species from which they were isolated, and to the molecular groups.

Colletotrichum isolates related to Anthracnose of cashew trees in Brazil: morphological and molecular description using LSU rDNA sequences

“…(Adaskaveg and Hartin, 1997;Lane et al, 1998;Menezes and Hanlin, 1996a, b;Moriwaki et al, 2002). According to the studies of Munaut et al (2001), although the majority of isolates of C. gloeosporioides from Stylosanthes formed straight spores, many produced falcate ones. Conidial length was considerably more variable than width.…”

“…In a study of C. gloeosporioides isolates from Stylosanthes spp. in Mexico, morphological and molecular criteria, including amplification and restriction of a specific fragment of the internal transcribed spacer 1 (ITS1) region of rDNA, were used to cluster the isolates into three groups partially related to their host species of origin (Munaut et al, 2001). Later, Abang et al (2002) failed to distinguish C. coccodes from C. circinans based on restriction analysis of their ITS sequence.…”

Variation among Colletotrichum gloeosporioides isolates from infected coffee berries at different locations in Vietnam

“…(2006) among 20 described isolates of C. gloeosporioides from coffee berries in Papau New Guinea. Munaut et al . (2001) reported conidia of C. gloeosporioides up to 29·5 µ m long isolated from Stylosanthes spp.…”

Morphological Characterization of Biocontrol Isolates of <i>Trichoderma</i> to Study the Correlation between Morphological Characters and Biocontrol Efficacy

“…This was based on the report by Samuel et al where he used the morphological characterization to analyze the variation between the isolates among Trichoderma species associated with green mold epidemic of commercially grown Agaricus bisporus [15]. Similarly Munaut et al reported variation in Colletotrichum gloeosporioides based on morphological characterization [12].The data were used to compute simple matching similarity index and dendrogram was constructed which is shown in Fig. 1.…”