Molecular and morphological characterization of the tapeworm<i>Taenia hydatigena</i>(Pallas, 1766) in sheep from Iran

Rostami, Sima; Salavati, Reza; Beech, Robin N.; Babaei, Zahra; Sharbatkhori, Mitra; Baneshi, Mohammad Reza; Hajialilo, Elham; Shad, Hamid Haddadian; Harandi, Majid Fasihi

doi:10.1017/s0022149x13000667

Cited by 41 publications

(37 citation statements)

References 30 publications

(32 reference statements)

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“…2). The weak nodal support observed between Nigerian haplotypes is similar to previous observations of low posterior probability values (0.51–1.00) and bootstrap values (79%) between T. hydatigena haplotypes from Iran and Tanzania [4, 27].…”

Section: Discussionsupporting

confidence: 88%

First molecular description, phylogeny and genetic variation of Taenia hydatigena from Nigerian sheep and goats based on three mitochondrial genes

et al. 2019

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BackgroundCysticercosis caused by the metacestode larval stage of Taenia hydatigena is a disease of veterinary and economic importance. A considerable level of genetic variation among isolates of different intermediate hosts and locations has been documented. Generally, data on the genetic population structure of T. hydatigena is scanty and lacking in Nigeria. Meanwhile, similar findings in other cestodes like Echinococcus spp. have been found to be of epidemiological importance. Our aim, therefore, was to characterize and compare the genetic diversity of T. hydatigena population in Nigeria based on three mitochondrial DNA markers as well as to assess the phylogenetic relationship with populations from other geographical regions.MethodsIn the present study, we described the genetic variation and diversity of T. hydatigena isolates from Nigerian sheep and goats using three full-length mitochondrial genes: the cytochrome c oxidase subunit 1 (cox1), NADH dehydrogenase subunit 1 (nad1), and NADH dehydrogenase subunit 5 (nad5).ResultsThe median-joining network of concatenated cox1-nad1-nad5 sequences indicated that T. hydatigena metacestodes of sheep origin were genetically distinct from those obtained in goats and this was supported by high FST values of nad1, cox1, and concatenated cox1-nad1-nad5 sequences. Genetic variation was also found to be higher in isolates from goats than from sheep.ConclusionsTo the best of our knowledge, the present study described the genetic variation of T. hydatigena population for the first time in Nigeria using full-length mitochondrial genes and suggests the existence of host-specific variants. The population indices of the different DNA markers suggest that analysis of long mitochondrial DNA fragments may provide more information on the molecular ecology of T. hydatigena. We recommend that future studies employ long mitochondrial DNA sequence in order to provide reliable data that would explain the extent of genetic variation in different hosts/locations and the biological and epidemiological significance.

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Section: Discussionsupporting

confidence: 88%

First molecular description, phylogeny and genetic variation of Taenia hydatigena from Nigerian sheep and goats based on three mitochondrial genes

et al. 2019

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“…The morphological characteristics of isolated cysts were consistent with those reported in the literature for T. hydatigena cysticerci, in different stages of maturation [33]. The morphological identification was confirmed by molecular biology and nucleotide sequences showed a 99% identity with T. hydatigena (GenBank: AB792722 and JN831270).…”

Section: Resultssupporting

confidence: 87%

“…Morphological identification of the parasites was performed according to the previously reported keys [33]. Molecular identification was then performed in order to confirm morphological diagnosis according to Scala et al [16].…”

Section: Methodsmentioning

confidence: 99%

Αcute visceral cysticercosis caused by Taenia hydatigena in lambs: ultrasonographic findings

et al. 2020

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Background Cysticercosis caused by cysticercus tenuicollis is a metacestode infection that affects several species of ungulates. It is caused by the larval stage of Taenia hydatigena, an intestinal tapeworm in dogs and wild canids. In the intermediate host, the mature cysticerci are usually found in the omentum, mesentery, and peritoneum, and less frequently in the pleura and pericardium. The migrating larvae can be found mostly in the liver parenchyma causing traumatic hepatitis in young animals. Most infections are chronic and asymptomatic, and are diagnosed at the abattoir. The acute form of infection is unusual in sheep and reports of death in lambs are rare. Methods In March 2018, fifteen female lambs presented anorexia, weakness, lethargy, and death secondary to acute visceral cysticercosis. Twelve of them underwent hepatic ultrasonography. Examinations were performed on standing or left lateral recumbent animals. Results Livers of affected animals presented rounded margins and a thickened, irregular and hyperechoic surface. Hepatic parenchyma appeared to be wholly or partially affected by lesions characterized by heterogeneous areas crossed by numerous, irregular, anechoic tracts ranging from 1 to 2 cm in length and 0.1 to 0.2 cm in width. Superficial and intraparenchymal cystic structures were also visualized. The presence of lesions was confirmed by anatomopathological examination, and T. hydatigena cysticerci was identified by morphological and molecular characterization of isolates. Conclusions Our results highlighted that hepatic ultrasonography is effective for an intra-vitam diagnosis of acute cysticercosis in lambs.

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“…Twenty isolates of each of three Taenia species, T. hydatigena, T. multiceps and T. ovis and 5 isolates of E. granulosus sensu stricto were collected from sheep during routine veterinary inspection from Tehran, Alborz and Kerman provinces. The parasites have already been sequenced and genetically characterized at the mitochondrial cytochrome C oxidase subunit 1 (CO1) 2 , 27 , 28 . Sequence data were deposited in GenBank with the following accession numbers: T. hydatigena (JQ710588), T. multiceps (JQ710577) and T. ovis (JX134111) and E. granulosus sensu stricto (KF443137).…”

Section: Methodsmentioning

confidence: 99%

A conventional PCR for differentiating common taeniid species of dogs based on in silico microsatellite analysis

Shamsaddini

Mohammadi

Mirbadie

et al. 2017

Rev. Inst. Med. trop. S. Paulo

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Canine taeniids are among the major tapeworms with remarkable medical and economic significance. Reliable diagnosis and differentiation of dog taeniids using simple and sensitive tools are of paramount importance for establishing an efficient surveillance system. Microsatellites as abundant unique tandem repeats of short DNA motifs are useful genetic markers for molecular epidemiological studies. The purpose of the present study was to find a primer pair for rapid differentiation of major tapeworms of dogs, Taenia hydatigena, T. multiceps, T. ovis and Echinococcus granulosus, by screening existing nucleotide data. All the mitochondrial genome records as well as non-coding ITS1 sequences of Taeniidae species were downloaded from Nucleotide database from NCBI. For prediction and analysis of potential loci of STR/SSR in ITS1 as well as mitochondrial regions, we used ChloroMitoSSRDB 2.0 and GMATo v1.2. software. Different tapeworm species were categorized according to different motif sequences and type and size of each microsatellite locus. Three primer sets were designed and tested for differentiating taeniid species and evaluated in a conventional PCR system. Four taeniid species were successfully differentiated using a primer pair in a simple conventional PCR system. We predicted 2-19 and 1-4 microsatellite loci in ITS1 and mitochondrial genome, respectively. In ITS1, 41 Di and 21 Tri motifs were found in the taeniids while the majority of the motifs in the mitochondrial genome were Tetra (89) and Tri (70). It is documented that the number and diversity of microsatellite loci is higher in nuclear ITS1 region than mostly coding mitochondrial genome.

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Molecular and morphological characterization of the tapewormTaenia hydatigena(Pallas, 1766) in sheep from Iran

Cited by 41 publications

References 30 publications

First molecular description, phylogeny and genetic variation of Taenia hydatigena from Nigerian sheep and goats based on three mitochondrial genes

First molecular description, phylogeny and genetic variation of Taenia hydatigena from Nigerian sheep and goats based on three mitochondrial genes

Αcute visceral cysticercosis caused by Taenia hydatigena in lambs: ultrasonographic findings

A conventional PCR for differentiating common taeniid species of dogs based on in silico microsatellite analysis

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