Molecular and Insecticidal Characterization of a Novel  Cry-Related Protein from Bacillus Thuringiensis Toxic against Myzus persicae

Palma, Leopoldo; Muñoz, Delia; Berry, Colin; Murillo, Jesús; Escudero, Íñigo Ruiz de; Caballero, Primitivo

doi:10.3390/toxins6113144

Cited by 42 publications

(48 citation statements)

References 37 publications

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“…Similar to this as reported by Palma et al (2014b), that describes the insecticidal activity of a novel B. thuringiensis Cry-related protein with a deduced 799 amino acid sequence (~89 kDa) and ~19% pairwise identity 95-kDa-aphidicidal protein (sequence number 204) and ~40% pairwise identity to the cancer cell killing Cry proteins (parasporins Cry41Ab1 and Cry41Aa1), respectively. This determined that SPE of B. thuringiensis induced cellular death through apoptosis.…”

Section: Discussionsupporting

confidence: 83%

In vitro antitumoral activity of soluble protein extracts of Bacillus thuringiensis

Franco‐Molina¹,

Mendoza-Gamboa²,

Roman-Calderon³

et al. 2016

Afr. J. Microbiol. Res.

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There are many studies about the antitumour effects of Bacillus toxins from different strains or subspecies in different parts of the world. Proteins that selectively kill tumor cells in vitro have potential as anticancer agents. The aim of this study was to evaluate the cytotoxic activity of soluble proteins extracts (SPE) from Mexican strains of B. thuringiensis on murine lymphoma L5178YR cell line. In vitro, L5178YR cells were treated with different concentrations of specific primers (SPE) from B. thuringiensis (0 to 39.85 g/mL) and cellular viability was evaluated by MTT method and orange acridine/ethidium bromide staining. The mechanism of cell death was evaluated through caspase-3 activation by flow cytometry and TUNEL assays. The study results shows that SPE from B. thuringiensis (GM1-3 h, GM1-24 h, GM1-48 h, GM18-3 h, GM18-24 h, GM18-48 h, HD512-3 h, HD512-24 h, and HD512-48 h) affected the cell viability of L5178YR in a dose-dependent manner which presented higher cytotoxic effect of SPE collected at 3 h independent of the strain used; and the 7% SDS-PAGE presented an electrophoretic profile of proteins in a range of 10 to 100 kDa of the SPE B. thuringiensis. The cytotoxicity is through a mechanism of apoptosis because the caspase-3 activation and TUNEL assays corroborated this result. In conclusion, SPE derived from early culture (3 h) of B. thuringiensis (Bt) GM1, GM18 and HD-512 have in vitro cytotoxic potential on murine lymphoma L5178YR cell line through a mechanism of cell death by apoptosis.

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Section: Discussionsupporting

confidence: 83%

In vitro antitumoral activity of soluble protein extracts of Bacillus thuringiensis

Franco‐Molina¹,

Mendoza-Gamboa²,

Roman-Calderon³

et al. 2016

Afr. J. Microbiol. Res.

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“…As discussed earlier, Cry15, Cry23, Cry33, Cry38, Cry45 (parasporin 4), Cry51, Cry60 and Cry64 all show features of the ETX_MTX2 family that includes the Mtx2 protein from Bs and the Clostridium epsilon toxin 18,19 . A very recent report says that, when applied together, the three-domain Cry toxin, Cry9Aa and the VIP, VIP3Aa, exhibited high insecticidal activity against an important insect pest, the Asiatic rice borer (Chilo suppressalis) 22 . Similarly, Cry 9 is known to have toxicity against some lepidopteran 14,23 .…”

Section: Discussionmentioning

confidence: 99%

Enterococcus durans with mosquito larvicidal toxicity against Culex quinquefasciatus, elucidated using a Proteomic and Metabolomic approach

Colvin

Dhuri

Verma

et al. 2020

Sci Rep

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Various bacteria from the Bacillus species have been used as pesticides against mosquito larvae for more than a decade. the prolonged use of these bacterial species by little alteration within their genome, using various permutations and combinations of mosquito-cidal toxins, has proven unsuccessful in controlling the mosquito population. in our current study we report Enterococcus sp. to be exhibiting similar kind of mosquito-cidal toxins alike those which are present in the mainly used Bacillus strains. three Enterococcus species were isolated on a rich media selective for gram-positive bacteria from the mid-gut of dead mosquito larvae which were collected from the wild locations within and around the city of Mumbai, india. their surface morphologies were studied by Scanning electron Microscopy (SEM) and their identity was confirmed using the standard 16S rRNA sequencing method. Upon performing several repetitive toxicity assays of these three strains on the laboratory cultured third instar stage of Culex quinquefasciatus larvae, showed differential toxicities from a minimum of 20% (LC 50 : 59.6 CFU/ml), intermediate 35% (LC 50 : 48.4 CFU/ml) and a maximum of 60% (LC 50 : 35.7 CFU/ ml). to justify the data in all the three similar strains of Enterococcus durans, we followed the differential proteomics using LCMS 6540 UHD Accurate Mass QTOF and differential metabolomics approach using both LCMS 6540 UHD Accurate Mass QTOF and 1 H-NMR. The presence and significance of the obtained toxins were studied to elucidate the plausible reason for showing differential toxicities. This work helped in identifying Enterococcus durans as a new, potential and alternative strain to the Bacillus species in terms of mosquito larvicidal toxicity against Culex quinquefasciatus.

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“…(2009) tested the toxins Cry3A, Cry4A, Cry11A, and Cyt1A against A. pisum and obtained LC50 values of 70 ng/µL for Cry3A and 100 ng/ µL for the rest of the toxins; similarly, Huarong et al (2011) showed that LC50 of Cry3A and Cry1A toxins against A. pisum where 500 ng/ µL for both toxins. Palma et al (2014) found a new B. thuringiensis protein that was toxic to M. persicae with an LC50 value of 32.7 ng/µL. Sattar & Maitti (2011) identified a homologous protein to Vip2A toxic to Aphis gossypii Glover (Hemiptera: Aphididae) with an LC50 value of 0.356 ng/µL.…”

Section: Discussionmentioning

confidence: 99%

Characterization ofBacillus thuringiensis(Bacillaceae) Strains Pathogenic toMyzus persicae(Hemiptera: Aphididae)

Torres-Quintero

Arenas-Sosa

Hernández-Velázquez

et al. 2016

Florida Entomologist

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Molecular and Insecticidal Characterization of a Novel Cry-Related Protein from Bacillus Thuringiensis Toxic against Myzus persicae

Cited by 42 publications

References 37 publications

In vitro antitumoral activity of soluble protein extracts of Bacillus thuringiensis

In vitro antitumoral activity of soluble protein extracts of Bacillus thuringiensis

Enterococcus durans with mosquito larvicidal toxicity against Culex quinquefasciatus, elucidated using a Proteomic and Metabolomic approach

Characterization ofBacillus thuringiensis(Bacillaceae) Strains Pathogenic toMyzus persicae(Hemiptera: Aphididae)

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