Molecular and in silico analysis of a new plasmid-mediated AmpC β-lactamase (CMH-2) in clinical isolates of Klebsiella pneumoniae

Ingti, Birson; Laskar, Monjur Ahmed; Choudhury, Sudip; Maurya, Anand Prakash; Paul, Deepjyoti; Talukdar, Anupam Das; Choudhury, Manabendra Dutta; Dhar, Debadatta; Chakravarty, Atanu; Bhattacharjee, Amitabha

doi:10.1016/j.meegid.2016.12.009

Cited by 8 publications

(4 citation statements)

References 25 publications

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“…However, the presence of bla MIR-like ampC genes on the plasmids among K. pneumoniae and E. cloacae highlights the capability of mobility of bla MIR between different germs of Enterobacteriaceae . Furthermore, a novel plasmid-mediated CMH-2 ampC gene with a sequence similarity of 98.6% to CMH-1 was recovered from two clinical K. pneumoniae isolates in India, suggesting continuous evolution and spreading of the bla CMH resistance trait [52].…”

Section: Discussionmentioning

confidence: 99%

Detection of Plasmid-Mediated β-Lactamase Genes and Emergence of a Novel AmpC (CMH-1) in Enterobacter cloacae at a Medical Center in Southern Taiwan

Lee

Chuang

et al. 2018

JCM

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The plasmid-mediated extended-spectrum β-lactamases (ESBLs) and AmpC β-lactamases in Enterobacter spp. have increasingly been reported. In this study, we investigated the prevalence of the plasmid-mediated β-lactamases in Enterobacter cloacae from bloodstream isolates at a medical center in southern Taiwan. ESBL and ampC genes were detected by PCRs and DNA sequencing. Conjugation experiments were conducted to confirm the transferability of the genetic resistance trait. Among 41 non-repetitive blood isolates of cefuroxime-resistant E. cloacae, eight isolates exhibited ESBL phenotype confirmed by double-disk synergistic tests. Nearly all the strains were susceptible to carbapenems. The prevalence rate of the plasmid-mediated blaampC genes was 73% (30/41), including one blaDHA-1, one blaMIR-6, two novel blaCMH-1 genes and other blaACT-like genes. Coexistence of plasmid-mediated blaACT and ESBL genes (10 with blaSHV-12 and one with blaCTX-M-3) was observed. Successful transmissions of the blaACT and blaCMH-1 were demonstrated in some transconjugants. The inducible or derepressed CMH-1 had expanded activity of isolates versus ceftazidime. Enterobacterial repetitive intergenic consensus (ERIC)-PCR analysis and pulsotype showed distinct patterns suggesting non-clonal relationship. In conclusion, plasmid-mediated blaACT-like ampC genes in E. cloacae isolates have been highly prevalent in southern Taiwan and may continue genetic evolution, contributing to the complexities in antibiotic-resistant mechanisms.

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Section: Discussionmentioning

confidence: 99%

Detection of Plasmid-Mediated β-Lactamase Genes and Emergence of a Novel AmpC (CMH-1) in Enterobacter cloacae at a Medical Center in Southern Taiwan

Lee

Chuang

et al. 2018

JCM

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“…Our analysis showed that bla CMH was the most frequent β-lactamase gene. However, literature search with bla CMH as the key word showed that bla CMH-1 was first detected in E. cloacae as a novel bla AmpC gene at a Medical Center in Southern Taiwan [22]. Since then, bla CMH-2 and bla CMH-3 were sequentially identified in India and Europe [22,23].…”

Section: Discussionmentioning

confidence: 99%

“…However, literature search with bla CMH as the key word showed that bla CMH-1 was first detected in E. cloacae as a novel bla AmpC gene at a Medical Center in Southern Taiwan [22]. Since then, bla CMH-2 and bla CMH-3 were sequentially identified in India and Europe [22,23]. Thereafter, no bla CMH was reported in PubMed database albeit genomic analysis showed the widest distribution of these enzyme.…”

Section: Discussionmentioning

confidence: 99%

Molecular characteristics of global β-lactamase-producing Enterobacter cloacae by genomic analysis

Liu

et al. 2022

BMC Microbiol

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Objective To analyze the characteristics of global β-lactamase-producing Enterobacter cloacae including the distribution of β-lactamase, sequence types (STs) as well as plasmid replicons. Methods All the genomes of the E. cloacae were downloaded from GenBank. The distribution of β-lactamase encoding genes were investigated by genome annotation after the genome quality was checked. The STs of these strains were analyzed by multi-locus sequence typing (MLST). The distribution of plasmid replicons was further explored by submitting these genomes to the genome epidemiology center. The isolation information of these strains was extracted by Per program from GenBank. Results A total of 272 out of 276 strains were found to carry β-lactamase encoding genes. Among them, 23 varieties of β-lactamase were identified, blaCMH (n = 130, 47.8%) and blaACT (n = 126, 46.3%) were the most predominant ones, 9 genotypes of carbapenem-hydrolyzing β-lactamase (CHβLs) were identified with blaVIM (n = 29, 10.7%) and blaKPC (n = 24, 8.9%) being the most dominant ones. In addition, 115 distinct STs for the 272 ß-lactamase-carrying E. cloacae and 48 different STs for 106 CHβLs-producing E. cloacae were detected. ST873 (n = 27, 9.9%) was the most common ST. Furthermore, 25 different plasmid replicons were identified, IncHI2 (n = 65, 23.9%), IncHI2A (n = 64, 23.5%) and IncFII (n = 62, 22.8%) were the most common ones. Notably, the distribution of plasmid replicons IncHI2 and IncHI2A among CHβLs-producing strains were significantly higher than theat among non-CHβLs-producing strains (p < 0.05). Conclusion Almost all the E. cloacae contained β-lactamase encoding gene. Among the global E. cloacae, blaCMH and blaACT were main blaAmpC genes. BlaTEM and blaCTX-M were the predominant ESBLs. BlaKPC, blaVIM and blaNDM were the major CHβLs. Additionally, diversely distinct STs and different replicons were identified.

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“…All sequencing results were compiled using DNA Dragon software 1 (Hepperle, 2011). The consensus sequences obtained from DNA Dragon were checked for their homology with reported sequences presenting nucleotide database using BLASTn and BLASTx programs of NCBI BLAST 2 (Ingti et al, 2017). The sequences showing significant similarity with query sequences were retrieved and aligned using online Multiple Sequence Alignment tool CLUSTALW 3 (Thompson et al, 1994).…”

Section: In Silico Analysismentioning

confidence: 99%

Isolation and characterization of peroxidase P7-like gene and Rab-GDI like gene from potential medicinal plants: A step toward understanding cell defense signaling

et al. 2022

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Defensin genes form part of a plant’s defense system and are activated when exposed to biotic or abiotic stress. They play a vital role in controlling many signaling pathways involved in various plant defense mechanisms. This research aimed to isolate and characterize novel defensin genes from selected medicinally important plants to explore their signaling mechanisms and defense associated roles for breeding. The DNA of Albizia lebbeck and Moringa oleifera was subjected to PCR amplification using gene-specific primers of defensin genes. Two novel defensin genes were isolated in each species, with sequence lengths of 300 bp in A. lebbeck and 150 bp in M. oleifera. In-silico analysis undertaken to retrieve and align their orthologous sequences revealed 100% similarity of the A. lebbeck gene with the Musa acuminate peroxidase P7-like gene and 85% similarity of the M. oleifera gene with the Manihot esculenta GDP dissociation inhibitor gene. The reliability, stability and physiochemical properties of homology models of these sequences was confirmed through online computational studies. This preliminary study confirmed the presence of novel genes with peroxidase P7 and Rab GDP dissociation inhibitor gene-like activity in A. lebbeck and M. oleifera, respectively, and their potential defense role in plants. Thus, the defensin genes of both species could be used in the synthesis of transgenic self-defensive plants with increased disease resistance and as potential candidates for improved crop production and thraputic formulation in the future.

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Molecular and in silico analysis of a new plasmid-mediated AmpC β-lactamase (CMH-2) in clinical isolates of Klebsiella pneumoniae

Cited by 8 publications

References 25 publications

Detection of Plasmid-Mediated β-Lactamase Genes and Emergence of a Novel AmpC (CMH-1) in Enterobacter cloacae at a Medical Center in Southern Taiwan

Detection of Plasmid-Mediated β-Lactamase Genes and Emergence of a Novel AmpC (CMH-1) in Enterobacter cloacae at a Medical Center in Southern Taiwan

Molecular characteristics of global β-lactamase-producing Enterobacter cloacae by genomic analysis

Isolation and characterization of peroxidase P7-like gene and Rab-GDI like gene from potential medicinal plants: A step toward understanding cell defense signaling

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