Molecular and immunological characterization of Pasteurella multocida serotype A:3 OmpA: evidence of its role in P. multocida interaction with extracellular matrix molecules

Dabo, S. M.; Confer, Anthony W.; Quijano-Blas, R.A.

doi:10.1016/s0882-4010(03)00098-6

Cited by 65 publications

(62 citation statements)

References 45 publications

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“…Amino acid diversity within the surface-exposed loops is clearly advanta- on May 7, 2018 by guest http://jb.asm.org/ geous to M. haemolytica and suggests that these parts of the molecule play an important role in some aspect of this pathogen's biology. An increasing body of evidence from other pathogens indicates that OmpA functions as a ligand, is involved in binding to specific host cell receptor molecules, and plays a role in adherence and colonization (9,24,33,42,44,46,55). The exclusive association of the OmpA1-and OmpA2-type proteins with bovine and ovine strains of M. haemolytica, respectively, together with evidence that the ompA1-and ompA2-type genes have undergone horizontal transfer between strains of divergent phylogenetic lineages, indicates that OmpA is under strong selective pressure from the host and plays an important role in host-pathogen relationships.…”

Section: Discussionmentioning

confidence: 99%

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Sequence Diversity and Molecular Evolution of the Heat-Modifiable Outer Membrane Protein Gene ( ompA ) of Mannheimia ( Pasteurella ) haemolytica , Mannheimia glucosida , and Pasteurella trehalosi

Davies

Lee

2004

J Bacteriol

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The OmpA (or heat-modifiable) protein is a major structural component of the outer membranes of gramnegative bacteria. The protein contains eight membrane-traversing ␤-strands and four surface-exposed loops. The genetic diversity and molecular evolution of OmpA were investigated in 31 Mannheimia (Pasteurella) haemolytica, 6 Mannheimia glucosida, and 4 Pasteurella trehalosi strains by comparative nucleotide sequence analysis. The OmpA proteins of M. haemolytica and M. glucosida contain four hypervariable domains located at the distal ends of the surface-exposed loops. The hypervariable domains of OmpA proteins from bovine and ovine M. haemolytica isolates are very different but are highly conserved among strains from each of these two host species. Fourteen different alleles representing four distinct phylogenetic classes, classes I to IV, were identified in M. haemolytica and M. glucosida. Class I, II, and IV alleles were associated with bovine M. haemolytica, ovine M. haemolytica, and M. glucosida strains, respectively, whereas class III alleles were present in certain M. haemolytica and M. glucosida isolates. Class I and II alleles were associated with divergent lineages of bovine and ovine M. haemolytica strains, respectively, indicating a history of horizontal DNA transfer and assortative (entire gene) recombination. Class III alleles have mosaic structures and were derived by horizontal DNA transfer and intragenic recombination. Our findings suggest that OmpA is under strong selective pressure from the host species and that it plays an important role in host adaptation. It is proposed that the OmpA protein of M. haemolytica acts as a ligand and is involved in binding to specific host cell receptor molecules in cattle and sheep. P. trehalosi expresses two OmpA homologs that are encoded by different tandemly arranged ompA genes. The P. trehalosi ompA genes are highly diverged from those of M. haemolytica and M. glucosida, and evidence is presented to suggest that at least one of these genes was acquired by horizontal DNA transfer.Mannheimia (Pasteurella) haemolytica is a commensal of cattle, sheep, and other ruminants, but it also causes bovine and ovine pneumonic pasteurellosis (infecting the respiratory tract), which is responsible for considerable economic losses to the cattle and sheep industries (6,20,21). The organism consists of genetically distinct subpopulations that are differentially adapted to and elicit disease in either cattle or sheep (11,14). M. haemolytica possesses various putative virulence determinants (23), including a transferrin receptor (38, 59) and a leukotoxin (26, 49) which are specific for ruminant transferrin (38, 59) and lymphoid cells (4, 7, 27, 49), respectively, and are thought to contribute to the organism's host specificity. However, the molecular basis of host adaptation in the bovine and ovine lineages of M. haemolytica remains largely unclear. Mannheimia glucosida was previously recognized as the A11 serotype of M. haemolytica and represents a heterogeneous group of organ...

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Section: Discussionmentioning

confidence: 99%

“…However, OmpA is also involved in adherence to host tissues in Chlamydia spp. (36), Escherichia coli (44,55), Haemophilus influenzae (24), and Pasteurella multocida (9). For E. coli and H. influenzae, the host cell receptor molecules have been identified (24,42,43).…”

mentioning

confidence: 99%

Sequence Diversity and Molecular Evolution of the Heat-Modifiable Outer Membrane Protein Gene ( ompA ) of Mannheimia ( Pasteurella ) haemolytica , Mannheimia glucosida , and Pasteurella trehalosi

Davies

Lee

2004

J Bacteriol

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“…The structural components on the surface of bacteria including outer membrane protein A (OmpA) have been incriminated a s virulence factors involved in pathogenesis as they mediate the colonization t o the host system (Dabo et al, 2003). The mechanisms involved in the pathogenesis of different Pasteurella multocida serogroups differ considerably as serogroups A and D cause fowl cholera a n d atrophic rhinitis, respectively, which are localized forms of infection, whereas serogroups B and .E are responsible for septicaemic conditions.…”

Section: Introductionmentioning

confidence: 99%

“…OmpA needs to be explored in detail as it plays a major role in facilitating the adhesion to the host upper respiratory tract mucosa. OmpA has been recently characterized in P. multocida serotype A: 3 and shown to perform a variety of functions like adherence to cell surface proteins, fibronectin and heparin, suggesting its role in bacterial-host relationship and therefore, in pathogenesis of P. multocida (Dabo et al, 2003). So far no work regarding OmpA has been carried out in serotype B: 2, the major cause of haemorrhagic septicaemia.…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization ofompAGene ofPasteurella multocidaSerotype B: 2

Singh¹,

Singh²,

Kumar³

et al. 2007

Journal of Applied Animal Research

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“…In common with other gram-negative bacteria, M, haemolytica possesses outer membrane protein A (OmpA), a heat-modifiable protein which is an integral component of the outer membrane (Beher et al 1980), with immunogenic activity (Ayalew et al 2011, Puohiniemi et al 1990, Zeng et al 1999. OmpA is also involved in adher ence to host tissues in related pathogens such as Pas teurella multocida (Dabo et al 2003). The adherence of respiratory pathogens to the mucosal epithelium is critical in host colonization and infection (Beachey 1981).…”

mentioning

confidence: 99%

Serotypic diversity and sequence variation of the ompA gene among Mannheimia haemofytica isolates from domestic ruminants

Vougidou

Sandalakis²,

Psaroulaki³

et al. 2017

J Hellenic Vet Med Soc

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Pneumonia caused by Mannheìmia haemolytica is an important disease of ruminants. Because of its economic significance, several methods have been used to study the pathogenicity and epidemiology of M. haemolytica. The objectives of this study were to provide data about the prevalence of the different serotypes of the bacterium and to investigate the genetic diversity of a significant virulence factor, the ompA gene. Two methods, DNA sequencing analysis and DGGli, were used to study the polymorphisms of the ompA gene. Ninety-four isolates from pneumonic lungs were investigated. Capsular scrotyping showed that serotype Λ2 was the predominant serotype among ovine strains and the only serotype found in caprine strains. This is the first reported analysis of the ompA gene of M haemolytica strains isolated from goats. Analysis of the gene revealed five DGGli patterns and nine genotypic groups. The ovine isolates, which belonged to four DC G li patterns, showed a much greater diversity than the caprine strains, which belonged to just two DGGF patterns, Sequence analysis was used to verify the DGGE results and revealed eight genotypic groups among the ovine isolates and three among the caprine ones. Furthermore, the correlation of these results showed a great diversity of the ompA gene among serotype A2 isolates.

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Molecular and immunological characterization of Pasteurella multocida serotype A:3 OmpA: evidence of its role in P. multocida interaction with extracellular matrix molecules

Cited by 65 publications

References 45 publications

Sequence Diversity and Molecular Evolution of the Heat-Modifiable Outer Membrane Protein Gene ( ompA ) of Mannheimia ( Pasteurella ) haemolytica , Mannheimia glucosida , and Pasteurella trehalosi

Sequence Diversity and Molecular Evolution of the Heat-Modifiable Outer Membrane Protein Gene ( ompA ) of Mannheimia ( Pasteurella ) haemolytica , Mannheimia glucosida , and Pasteurella trehalosi

Molecular Characterization ofompAGene ofPasteurella multocidaSerotype B: 2

Serotypic diversity and sequence variation of the ompA gene among Mannheimia haemofytica isolates from domestic ruminants

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