Molecular and immunological characterization of novel weed pollen pan‐allergens

Wopfner, Nicole; Gruber, Petra; Wallner, Michael; Briza, Peter; Ebner, Christof; Mari, Adriano; Richter, Klaus; Vogel, Lothar; Ferreira, Fátima

doi:10.1111/j.1398-9995.2008.01635.x

Cited by 47 publications

(45 citation statements)

References 28 publications

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“…Amb a 8 has been found to have an 89% amino acid sequence identity with Art v 4 (4). In addition to their sequence identity, both Art v 4 and Amb a 8 have comparable IgE cross-reactivity, suggesting that either allergen could be used as a marker for profilin sensitization in weeds (4). Moreover, we compared molecular and immunological properties of Amb a 8 and Art v 4 with those of Bet v 2, a birch pollen profilin.…”

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confidence: 99%

“…Short ragweed, as well as giant ragweed, acts as a major elicitor of type 1 allergenic reactions in late summer and early fall, affecting over 15 million people in the United States and Canada (15). Amb a 8 has been found to have an 89% amino acid sequence identity with Art v 4 (4). In addition to their sequence identity, both Art v 4 and Amb a 8 have comparable IgE cross-reactivity, suggesting that either allergen could be used as a marker for profilin sensitization in weeds (4).…”

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confidence: 99%

“…Due to this cross-recognition, panallergens are thought to be indicators of sensitization to several pollens (2,3). Therefore, it is possible that panallergens could be used to predict cross/ poly-sensitization to a variety of pollen allergens (4).…”

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confidence: 99%

“…Only a few protein families contain panallergens, including calcium-binding proteins (4), cyclophilins (5), nonspecific lipid transfer proteins, pathogenesis-related protein (PR-10), polcalcins, and profilins (1). Profilins, which are the focus of this study, are small (12-15 kDa), mostly eukaryotic proteins that are involved in regulating the actin cytoskeleton (6).…”

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confidence: 99%

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Structural, Functional, and Immunological Characterization of Profilin Panallergens Amb a 8, Art v 4, and Bet v 2

Offermann

Schlachter

Perdue

et al. 2016

Journal of Biological Chemistry

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confidence: 99%

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confidence: 99%

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confidence: 99%

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Structural, Functional, and Immunological Characterization of Profilin Panallergens Amb a 8, Art v 4, and Bet v 2

Offermann

Schlachter

Perdue

et al. 2016

Journal of Biological Chemistry

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“…The clinical relevance of Unauthenticated Download Date | 5/8/18 8:27 AM polcalcin sensitization is linked to geographical factors and to the level of exposure to different allergenic sources. It has been shown that among weed pollenallergic patients, IgE reactivity to polcalcins from mugwort and ragweed, is much higher in Italians (21 to 28%) when compared to Austrians (10%) 29 . Monitoring of polcalcin-sensitized patients should be performed as these individuals are at risk of developing multiple pollen sensitizations 23 .…”

Section: Polcalcins and Allergic Rhinitismentioning

confidence: 99%

Polcalcins as pollen panallergens in allergic rhinitis

Popescu

Vieru

2016

Romanian Journal of Rhinology

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Polcalcins are highly cross-reactive calcium-binding allergen components specifically expressed in pollen from trees, grasses and weeds. The grass allergen component rPhl p 7, a recombinant non-glycosylated calcium-binding protein of 2-EF-hand type, is the most cross-reactive polcalcin and may be used as a polcalcin biomarker of IgE-mediated hypersensitivity. Polcalcin sensitization, which appears to be linked to geographical factors, level and time of pollen exposure, has to be assessed in allergic rhinitis patients with multiple pollen sensitizations and may be useful for a better targeted prescription of allergen immunotherapy.

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Complex IgE sensitization patterns in ragweed allergic patients: Implications for diagnosis and specific immunotherapy

Buzan

Zbîrcea

Gattinger

et al. 2022

Clinical & Translational All

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Background Ragweed (Ambrosia artemisiifolia) is one of the most important allergen sources, worldwide, causing severe respiratory allergic reactions in late summer and fall, in sensitized patients. Amb a 1 has been considered as the most important allergen in ragweed but 12 ragweed pollen allergens are known. The aim of our study was to investigate IgE reactivity profiles of ragweed allergic patients and to associate them with clinical symptoms. Methods IgE sensitization profiles from clinically well‐characterized ragweed allergic patients (n = 150) were analyzed using immunoblotted ragweed pollen extract. Immunoblot inhibition experiments were performed with two Amb a 1 isoforms and CCD markers and basophil activation experiments were performed with IgE serum before and after depletion of Amb a 1‐specific IgE. Results By IgE‐immunoblotting 19 different IgE reactivity patterns with and without Amb a 1‐sensitization were found. The majority of patients (>95%) suffered from rhino‐conjunctivitis, around 60% reported asthma‐like symptoms and about 25% had skin reactions. Patients with complex IgE sensitization profiles tended to have more clinical symptoms. Serum with and without Amb a 1‐specific IgE induced basophil activation. Conclusions Ragweed pollen allergic patients exhibit complex IgE reactivity profiles to ragweed allergens including Amb a 1 isoforms and cross‐reactive carbohydrates indicating the importance of Amb a 1 isoforms and additional allergens for diagnosis and allergen‐specific immunotherapy of ragweed allergy.

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Molecular and immunological characterization of novel weed pollen pan‐allergens

Abstract: The identified proteins are novel pan-allergens and can be used as diagnostic markers for polysensitization and used in component-resolved diagnosis.

Cited by 47 publications

References 28 publications

Structural, Functional, and Immunological Characterization of Profilin Panallergens Amb a 8, Art v 4, and Bet v 2

Structural, Functional, and Immunological Characterization of Profilin Panallergens Amb a 8, Art v 4, and Bet v 2

Polcalcins as pollen panallergens in allergic rhinitis

Complex IgE sensitization patterns in ragweed allergic patients: Implications for diagnosis and specific immunotherapy

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