Molecular and histopathological characterization of lumpy skin disease in cattle in northern Vietnam during the 2020–2021 outbreaks

Trinh, Thi Bich Ngoc; Nguyen, Van Tam; Nguyễn, Thủy Thu; Anh, Nguyễn Tuấn; Le, Phuong N.; Lai, Thi Ngoc Ha; Phan, Thanh Huong; Tran, Duc Hoan; Pham, Ngoc Thach; Dam, Van Phai; Nguyễn, Thị Lan; Ambagala, Aruna; Babiuk, Shawn; Le, Van Phan

doi:10.1007/s00705-022-05533-4

Cited by 11 publications

(10 citation statements)

References 33 publications

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“…LSD has been listed a noti able disease under the guidelines of the OIE, the clinical symptoms including high fever, drop in milk production, mastitis, emaciation, and even death in cattle [13]. Since the rst outbreak of LSD documented in Xinjiang region of China in 2019 [15], this disease has been reported in more than ten provinces or regions of China (including Anhui, Fujian, Guangdong, Inner Monglia, Jiangxi, Guangdong, Anhui, Zhejiang, Taiwan, Ningxia, Shandong, and Hongkong) [16,17].…”

Section: Discussionmentioning

confidence: 99%

“…The GPCR and and p32 genes are suitable targets for genetic analysis of LSDV strains [13]. In this study, the complete p32 and GPCR sequences of six LSDV strains obtained here were ampli ed and analyzed.…”

Section: Discussionmentioning

confidence: 99%

“…Yunnan province shares a long border with three Asian countries (Vietnam, Myanmar, and Laos). The rst outbreak of LSD in Vietnam, Myanmar, and Laos was documented in October 2020 [13], November 2020 [28], and 2011 [29], respectively. Therefore, the source of LSD outbreaks in Yunnan province needs further investigations.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, LSDV is antigenically correlated with these two viruses, the GTPV or SPPV derived vaccines can provide cross protection against LSDV infection [10,11]. Among the LSDV genome, the complete p32 and GPCR genes are widely used for investigating the genetic and phylogenetic characteristics of LSDV strains [5,12,13].…”

mentioning

confidence: 99%

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Epidemiology and genetic characteristics of the lumpy skin disease virus in Yunnan province, China from 2019 to 2023

Yao,

Lin,

Zhu

et al. 2024

Preprint

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Background Lumpy skin disease (LSD) is caused by the lumpy skin disease virus (LSDV) and has emerged as a significant threat to the cattle and water buffalo industries in China. Investigating the epidemiological characteristics of LSDV will be beneficial to the control and prevention of this disease. In this study, a Taqman-Probe-Based real-time PCR (qPCR) assay was developed for detecting LSDV nucleotides, 2495 tissue or blood samples were collected from Yunnan province and tested using the established qPCR assay. The GPCR and p32 genes of the LSDV-positive samples were amplified, sequenced, and analyzed. Additionally, an infectious LSDV strain was isolated from one positive sample, and its biological characteristics were explored. Results The established qPCR assay demonstrated high sensitivity with a limit of detection of 4.83 copies/µL. Out of the 2495 collected specimens, six (0.24%) samples were positive for LSDV nucleotides, and all positive samples belonged to the skin scab samples. Molecular and phylogenetic analysis based on the P32 and GPCR genes revealed that six LSDV strains were genetically similar to field LSDV strains from China, Russia, and Thailand. One LSDV strain was isolated and replicated well in PST cells, while which was not sensitive to the MDBK cells. Conclusions This study provides the first report on the epidemiological characteristics of LSDV in Yunnan province of China, which confirmed the prevalence of LSDV in Cattle in this province. These findings will contribute to the development of effective strategies for the prevention and control of LSDV.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Epidemiology and genetic characteristics of the lumpy skin disease virus in Yunnan province, China from 2019 to 2023

Yao,

Lin,

Zhu

et al. 2024

Preprint

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“…LSD first occurred in Asia and the Pacific region in 2019 in northwest China, Bangladesh and India, followed by Vietnam and Taiwan (Lu et al., 2021; Sudhakar et al., 2021; Tran et al., 2021 Tsai et al., 2022; Trinh et al., 2022). While India reported outbreaks caused by a Kenyan vaccine strain, China reported a recombinant strain whose mosaic backbone is composed of a Neethling vaccine strain and a Kenyan vaccine strain (Sethi et al., 2022; Wang et al., 2022).…”

Section: Introductionmentioning

confidence: 99%

Genetic characterization and epidemiological analysis of the first lumpy skin disease virus outbreak in Mongolia, 2021

Sprygin

Sainnokhoi

Gombo-Ochir

et al. 2022

Transbounding Emerging Dis

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Novel lumpy skin disease virus (LSDV) strains of recombinant origin are on the rise in South East Asia following the first emergence in 2017, and published evidence demonstrates that such genetic lineages currently dominate the circulation. Mongolia reported first LSD outbreaks in 2021 in a north-eastern region sharing borders with Russia and China. For each of 59 reported LSDV outbreaks, the number of susceptible animals ranged from 8 to 8600 with a median of 572, while the number of infected animals ranged from one to 355 with a median of 14. Phylogenetic inferences revealed a close relationship of LSDV Mongolia/2021 with recombinant vaccine-like LSDV strains from Russia, China, Taiwan, Thailand and Vietnam. These findings support the published data that the circulating strain of LSDV belongs to the dominant recombinant lineage recently established in the region.

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Deciphering the genetic landscape of lumpy skin disease: Unraveling variable virulence through comprehensive genome sequence analysis in India

Mishra,

Biswas,

Nandi

et al. 2024

Virology

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Molecular and histopathological characterization of lumpy skin disease in cattle in northern Vietnam during the 2020–2021 outbreaks

Cited by 11 publications

References 33 publications

Epidemiology and genetic characteristics of the lumpy skin disease virus in Yunnan province, China from 2019 to 2023

Epidemiology and genetic characteristics of the lumpy skin disease virus in Yunnan province, China from 2019 to 2023

Genetic characterization and epidemiological analysis of the first lumpy skin disease virus outbreak in Mongolia, 2021

Deciphering the genetic landscape of lumpy skin disease: Unraveling variable virulence through comprehensive genome sequence analysis in India

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