Molecular and genetic description of a new hypomorphic mutation of Trithorax-like gene and analysis of its effect on Drosophila melanogaster oogenesis

Ogienko, A. G.; Karagodin, Dmitriy A.; Pavlova, Nadejda V.; Федорова, С. А.; Voloshina, M. V.; Баричева, Е. М.

doi:10.1134/s1062360408020070

Cited by 12 publications

(29 citation statements)

References 22 publications

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“…It has previously been shown that some Trl mutations lead to female sterility due to serious defects during oogenesis (Bhat et al ., ; Ogienko et al ., ). By using different combinations of Trl mutations in this study, we have determined that mutant males also exhibit a significant decline in fertility and mature sperm production.…”

Section: Resultsmentioning

confidence: 97%

GAGA protein is essential for male germ cell development in Drosophila

Дорогова

Федорова

Болоболова

et al. 2014

Genesis

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The Drosophila Trithorax-like (Trl) gene encodes a GAGA factor which regulates a number of developmentally important genes. In this study, we identify a new function for Drosophila GAGA factor in male germ cell development. Trl mutants carrying strong hypomorphic alleles display loss of primordial germ cells during their migration in embryogenesis and severe disruption in mitochondria structure during early spermatogenesis. The mutation resulted in small testes formation, a deficit of germ cells, abnormal mitochondrial morphogenesis, spermatocyte death through autophagy, and partial or complete male sterility. Pleiotropic mutation effects can be explained by the misexpression of GAGA factor target genes, the products of which are required for germ cell progression into mature sperm.

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Section: Resultsmentioning

confidence: 97%

GAGA protein is essential for male germ cell development in Drosophila

Дорогова

Федорова

Болоболова

et al. 2014

Genesis

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“…The Trl 362 mutation contains a P‐element insertion and an adjacent deletion that also remove some transcription initiation sites (Figure ). The Trl en82 mutation contains a 1.4 kb P‐element insertion near the first transcription start site (Ogienko et al, ). Normally, there are 2 main Trl transcripts of about 2.5 and 3.0 kb (Figure a,b) in Drosophila ovaries.…”

Section: Resultsmentioning

confidence: 99%

“…In Drosophila , in addition to Hox genes, GAF regulates a wide‐range of genes that are important for the formation of various organs and tissues at all stages of ontogenesis. It has been shown that GAF is necessary for embryogenesis, oogenesis, spermatogenesis, and eye and wing development (Bayarmagnai, Nicolay, Islam, Lopez‐Bigas, & Frolov, ; Bhat et al, ; Dos‐Santos et al, ; Ogienko et al, ; Ogienko et al, ; Omelina, Pavlova, Ogienko, & Baricheva, ). GAF is also involved in processes responsible for cellular memory, migration, and programmed cell death (Cavalli & Paro, ; Dorogova, Fedorova, Bolobolova, Ogienko, & Baricheva, ; Dorogova, Khrushcheva, Fedorova, Ogienko, & Baricheva, ; Mishra, Chopra, Srinivasan, & Mishra, ; Ogienko et al, ; Poux, Horard, Sigrist, & Pirrotta, ).…”

Section: Introductionmentioning

confidence: 99%

“…Previously, we showed that our originally generated mutations in Trl gene resulted in female sterility and caused a number of disruptions during oogenesis, including an abnormal number of oocytes and nurse cells, defects in cytoplasmic actin filament formation leading to rapid transport disorder, and failure of migration in different types of somatic cells (Ogienko et al, , , ). The range of abnormalities in oogenesis that we described earlier has a negative effect on oocyte formation, leading to a decrease in female fertility.…”

Section: Introductionmentioning

confidence: 99%

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GAGA protein is required for multiple aspects of Drosophila oogenesis and female fertility

Федорова

Дорогова

Болоболова

et al. 2018

Genesis

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Summary Investigation of Drosophila oogenesis provides the opportunity to understand conservative genetic mechanisms underlying fertile female gamete development. In this study, we showed that the Drosophila DNA‐binding protein GAGA factor (GAF) had a multifunctional role in oogenesis and it is involved in the regulation of this process genetic program. We studied the influence on Drosophila oogenesis of a number of mutations in the 5′ region of the Trl gene that encodes GAF. We found that our originally generated Trl mutations lead to a decrease in transcriptional gene activity and levels of GAF expression in both germline and follicular cells. Cytological (fluorescence and electron microscopy) analysis showed that GAF loss resulted in multiple oogenesis defects. Mutations affected the actin cytoskeleton, leading to decrease of cytoplasmic filaments in nurse cells and basal actin in follicular cells. GAF depletion also leads to abnormal follicular cells migration, both border and centripetal. In addition, mutant ovaries demonstrated abnormalities in germ cells, including mitochondria, endoplasmic reticulum, karyosome organization, yolk granule formation and selective transport. Loss of GAF also promoted excessive cell death and egg chamber degradation. In sum, these defects caused very high or full female sterility. Since one of the main GAF activities is regulation of transcription, the complex phenotypes of the Trl mutants might be the consequence of its multiple target genes misexpression.

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“…However, we have shown that blocking of RCs by the NC nuclei appeared only in 30.6% ( n = 36, P < 0.001) of cpb mutant egg chambers at stages 10B‐11 and only one canal (rarely 2) of 15 was typically blocked. In singed , chickadee , quail and Trithorax‐like mutants disturbance of cytoplasmic AFs formation is considered the main reason for the ‘dumpless’ phenotype (Cooley et al, ; Cant et al, ; Mahajan‐Miklos and Cooley, ; Ogienko et al, ). In most old egg chambers of these mutants, the nuclei of the four NCs directly attach to the oocyte, forming a cluster, while individual nuclei often appear to stretch partially into the oocyte, blocking the RCs.…”

Section: Resultsmentioning

confidence: 99%

Capping protein beta is required for actin cytoskeleton organisation and cell migration during Drosophila oogenesis

Ogienko

Карагодин

Lashina

et al. 2013

Cell Biology International

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Capping protein (CP) is a well-characterised actin-binding protein important for regulation of actin filament (AF) assembly. CP caps the barbed end of AFs, inhibiting the addition and loss of actin monomers. In Drosophila melanogaster, the gene encoding CP β-subunit is named capping protein beta (cpb; see Hopmann et al. [1996] J Cell Biol 133: 1293-305). The cpb level is reduced in the Drosophila bristle actin cytoskeleton and becomes disorganised with abnormal morphology. A reduced level of the CP protein in ovary results in disruption of oocyte determination, and disturbance of nurse cell (NC) cortical integrity and dumping. We describe novel defects appearing in cpb mutants during oogenesis, in which cpb plays an important role in border and centripetal follicle cell migration, ring canal development and cytoplasmic AF formation. The number of long cytoplasmic AFs was dramatically reduced in cpb hypomorphs and abnormal actin aggregates was seen on the inner side of NC membranes. A hypothesis to explain the formation of abnormal short-cut cytoplasmic AFs and actin aggregates in the cpb mutant NCs was proffered, along with a discussion of the reasons for 'dumpless' phenotype formation in the mutants.

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Molecular and genetic description of a new hypomorphic mutation of Trithorax-like gene and analysis of its effect on Drosophila melanogaster oogenesis

Cited by 12 publications

References 22 publications

GAGA protein is essential for male germ cell development in Drosophila

GAGA protein is essential for male germ cell development in Drosophila

GAGA protein is required for multiple aspects of Drosophila oogenesis and female fertility

Capping protein beta is required for actin cytoskeleton organisation and cell migration during Drosophila oogenesis

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