Molecular and genetic characterization of carbapenemase-producing bacteria in Venezuela

Gómez-Gamboa, Liliana; Barrios-Camacho, Humberto; Dúran-Bedolla, Josefina; Sánchez-Pérez, Alejandro; Reyna-Flores, Fernando; Perozo-Mena, Armindo; Zabala, Irene; Rodríguez-Medina, Nadia; Martínez-Barnetche, Jesús; Téllez-Sosa, Juan; Valdovinos-Torres, Humberto; Garza–Ramos, Ulises

doi:10.1080/1120009x.2019.1607452

Cited by 7 publications

(5 citation statements)

References 18 publications

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“…In Venezuela, KPC-producing K. pneumoniae and E. cloacae isolates were first described between 2009 and 2010 [4]. We found a clear predominance (77.9%) of K. pneumoniae complex KPC producers, as observed in previous studies describing isolates in Venezuela [6,20].…”

Section: Discussionsupporting

confidence: 86%

“…During the past years, the rates of infections by ESBL-producing isolates have greatly increased. As observed in a previous study in Venezuela, 63.64% of K. pneumoniae isolates were ESBLs producers [6].…”

Section: Discussionsupporting

confidence: 73%

“…strain was detected in 2002 [10]. More recently, A. baumannii harboring the bla OXA-23 gene (87.5%) and the bla NDM-1 gene (37.5%) were detected in Venezuela and the coexistence of bla OXA-23 /bla NDM-1 genes was identified in two of the isolates [6].…”

Section: Discussionmentioning

confidence: 98%

“…Infections caused by carbapenemase producers are associated with high rates of morbidity and mortality, limited therapeutic options for the treatment, prolonged hospital stays, and overall increased healthcare costs. Therefore, infection prevention remains one of the critical approaches for preventing the spread of such organisms [6,25].…”

Section: Discussionmentioning

confidence: 99%

“…In Venezuela, sporadic cases of CPB have been reported; KPC (Klebsiella pneumoniae carbapenemase)-producing K. pneumoniae and Enterobacter cloacae were first described between 2009 and 2010 [4] and K. pneumoniae harboring bla VIM (Verona Integron-encoded Metallo-β-lactamase)-type were reported in 2008 [5]. Currently KPC-2 and NDM-1 (New Delhi metallo-β-lactamase) are the genes which have been predominantly identified in K. pneumoniae [6]. VIM-2 was identified for first time in 2002 in isolates of Pseudomonas aeruginosa [7] and IMP (Imipenemase)-producing P. aeruginosa was described in 2013 [8].…”

Section: Introductionmentioning

confidence: 99%

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Detection of carbapenemase-producing bacteria in a public healthcare center from Venezuela

Gómez-Gamboa

Perozo-Mena

Bermudez-Gonzalez

et al. 2020

J Infect Dev Ctries

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Introduction: The dramatic increase in the prevalence and clinical impact of infections caused by Carbapenemase-Producing Bacteria in the nosocomial setting in Latin America represents an emerging challenge to public health. The present study detected carbapenemase-producing Gram-negative bacteria in patients from a Hospital from Venezuela, by phenotypic and genotypic methods. Methodology: The bacterial identification was carried out using conventional methods. The resistance to carbapenems was performed by Kirby-Baüer disk diffusion method, according to CLSI recommendations. The modified Hodge Test, double-disk with phenylboronic acid, double-disk with EDTA and Blue Carba Test were performed to detect phenotypic carbapenemase producers. The carbapenemase-encoding genes blaKPC, blaVIM, blaIMP, blaOXA-2, blaOXA-3, blaOXA-15 and blaOXA-21 were determined. Results: The bacterial species identified were Klebsiella pneumoniae complex (181), Pseudomonas aeruginosa (51), and Acinetobacter baumannii-calcoaceticus complex (119). KPC-type was detected in 40.17% of isolates and VIM-type in 14.53%. KPC-type gene was only identified in K. pneumoniae isolates (77.9%). VIM-type gene was identified in P. aeruginosa (86.27%) and K. pneumoniae isolates (3.87%). There was not detection of IMP-type and OXA-type genes. Conclusions: We found a predominance of K. pneumoniae KPC producers and a high rate of VIM-producing P. aeruginosa. The epidemiology of CPB in Venezuela is rapidly evolving, and enhanced surveillance and reporting are needed across the healthcare continuum.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionsupporting

confidence: 73%