Molecular and genetic analysis of resistance to METI-I acaricides in Iranian populations of the citrus red mite Panonychus citri

Abstract

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The H92R substitution in PSST is a reliable diagnostic biomarker for predicting resistance to mitochondrial electron transport inhibitors of complex I in European populations of Tetranychus urticae

“…Lastly, the A94V substitution in PSST of the citrus red mite P. citri was of interest to Alavijeh et al . as it is located only two amino acids away from H92R, while it was present with higher allele frequencies in a moderately resistant Iranian population 43 . In the present extensive screening of T. urticae populations, A94V was not detected.…”

“…additionally suggested the involvement of A94V in the related mite Panonychus citri . All field populations were screened for these target‐site mutations via sequencing of DNA from pooled mites per population 43 . Four different non‐synonymous mutations were observed in the PSST gene: the H92R (CAC ‐ > CGC) mutation, which appeared to be fixed in five populations (FP18‐4, FP18‐3, FP18‐5, FP19‐8 and FP19‐22) while it was segregating in five additional populations (FP20‐19, FP20‐21, FP18‐7, FP19‐15 and FP19‐5) with frequencies over 45% (Fig.…”

Identification and characterization of striking multiple‐insecticide resistance in a Tetranychus urticae field population from Greece

“…Moderate to high RRs to METI‐I acaricides have been reported previously 13,28,68 . A point mutation in psst/NDUFS7 of complex I has previously been associated with resistance to METI‐Is in both Tetranychus and Panonychus species, 9,69,70 and this mutation was also identified in the Trz population, although not fixed. In addition, an A55T substitution was also uncovered, but this mutation was not in a conserved region or near the binding site of METI‐Is.…”

Selectivity and molecular stress responses to classical and botanical acaricides in the predatory mite Phytoseiulus persimilisAthias‐Henriot (Acari: Phytoseiidae)

“…The de novo assembly, transcript annotation, and differential expression analysis were performed according to Alavijeh et al ., 45 with minor deviations from the pipeline. In an attempt to estimate the number of reads sufficient to create a complete assembly of the P. persimilis transcriptome, we subsampled a random selection of 2.5 and 5 million reads per replicate, totaling 40 and 80 million reads.…”