Molecular and functional resemblance of differentiated cells derived from isogenic human iPSCs and SCNT-derived ESCs

Zhao, Ming; Chen, Haodong; Liu, Qing; Shao, Ning; Sayed, Nazish; Wo, Hung Ta; Zhang, Joe Z.; Ong, Sang Ging; Li, Chun; Kim, Young-Kyun; Yang, Huaxiao; Chour, Tony; Ma, Hong; Gutiérrez, Nuria Martí; Karakikes, Ioannis; Mitalipov, Shoukhrat; Snyder, M; Wu, Joseph C.

doi:10.1073/pnas.1708991114

Cited by 66 publications

(49 citation statements)

References 42 publications

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“…Functional annotation clustering was performed on protein-coding genes upregulated by more than 5-fold in the EoE sample set (false discovery rate, <0.1). [24][25][26] Quantitative RT-PCR assays RNA was isolated from esophageal biopsy specimens for quantitative realtime RT-PCR, as described previously, 14 by using TaqMan Gene Expression Assays (Thermo Fisher Scientific, Waltham, Mass) for LOX (Hs00942483) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH; Hs99999905_m1), the latter as an internal control gene.…”

Section: Rna-sequencing Data Analysesmentioning

confidence: 99%

Fibrostenotic eosinophilic esophagitis might reflect epithelial lysyl oxidase induction by fibroblast-derived TNF-α

Kasagi¹,

Dods²,

Wang³

et al. 2019

Journal of Allergy and Clinical Immunology

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(to K.A.W. and H.N.). J.M.S. is funded by the Stuart Starr Endowed Chair, Food Allergy Research and Education. J.M.S. and A.B.M. are funded in part by the Consortium of Eosinophilic Gastrointestinal Disease Researchers (CEGIR). CEGIR (U54 AI117804) is part of the Rare Diseases Clinical Research Network (RDCRN), an initiative of the Office of Rare Diseases Research (ORDR), National Center for Advancing Translational Sciences (NCATS), and is funded through collaboration between the National Institute of Allergy and Infectious Diseases (NIAID), NIDDK, and NCATS. CEGIR is also supported by patient advocacy groups, including APFED, CURED, and EFC. Disclosure of potential conflict of interest: The authors declare that they have no relevant conflicts of interest.

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Section: Rna-sequencing Data Analysesmentioning

confidence: 99%

Fibrostenotic eosinophilic esophagitis might reflect epithelial lysyl oxidase induction by fibroblast-derived TNF-α

Kasagi¹,

Dods²,

Wang³

et al. 2019

Journal of Allergy and Clinical Immunology

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“…A previous report indicated that the ability of mouse NT-ESCs to form hematopoietic colonies is greater than the corresponding capacity of isogenic iPSCs 32 . Recently, Zhao et al 33 demonstrated the functional similarity of cardiomyocytes and endothelial cells derived from isogenic human iPSCs and NT-ESCs. However, both prior studies only evaluated the cell fate potential of the mesodermal lineage.…”

Section: Discussionmentioning

confidence: 99%

Reprogramming mechanisms influence the maturation of hematopoietic progenitors from human pluripotent stem cells

et al. 2018

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Somatic cell nuclear transfer (SCNT) or the forced expression of transcription factors can be used to generate autologous pluripotent stem cells (PSCs). Although transcriptomic and epigenomic comparisons of isogenic human NT-embryonic stem cells (NT-ESCs) and induced PSCs (iPSCs) in the undifferentiated state have been reported, their functional similarities and differentiation potentials have not been fully elucidated. Our study showed that NT-ESCs and iPSCs derived from the same donors generally displayed similar in vitro commitment capacity toward three germ layer lineages as well as proliferative activity and clonogenic capacity. However, the maturation capacity of NT-ESC-derived hematopoietic progenitors was significantly greater than the corresponding capacity of isogenic iPSC-derived progenitors. Additionally, donor-dependent variations in hematopoietic specification and commitment capacity were observed. Transcriptome and methylome analyses in undifferentiated NT-ESCs and iPSCs revealed a set of genes that may influence variations in hematopoietic commitment and maturation between PSC lines derived using different reprogramming methods. Here, we suggest that genetically identical iPSCs and NT-ESCs could be functionally unequal due to differential transcription and methylation levels acquired during reprogramming. Our proof-of-concept study indicates that reprogramming mechanisms and genetic background could contribute to diverse functionalities between PSCs.

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“…As iPSCs are derived from adult tissue rather than embryonic tissue, they avoid the ethical issues associated with ESCs. No significant differences in gene expression levels, surface marker expression, and morphology between ESCs and iPSCs are observed in cells from the same genetic background [131,132]. In addition to circumventing ethical controversies, another advantage of iPSCs over ESCs is that they can be obtained from donors of known disease phenotypes, which can be used for patientspecific disease models and drug screening.…”

Section: Stem Cell Engineeringmentioning

confidence: 99%

Organ-on-a-chip: recent breakthroughs and future prospects

et al. 2020

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BackgroundMicrofluidics is a science and technology that precisely manipulates and processes microscale fluids. It is commonly used to precisely control microfluidic (10 −9 to 10 −18 L) fluids using channels that range in size from tens to hundreds of microns and is known as a "lab-on-a-chip" [1][2][3][4]. The microchannel is small, but has a large surface area and high mass transfer, favoring its use in microfluidic technology applications including low regent usage, controllable volumes, fast mixing speeds, rapid responses, and precision control of physical and chemical properties [1,5,6]. Microfluidics integrate sample preparation, reactions, separation, detection, and basic operating units such as cell culture, sorting and cell lysis [7]. For these reasons, interest in OOAC has intensified [8]. OOAC combines a range of chemical, biological and material science disciplines [9] and was selected as one of the "Top Ten Emerging Technologies" in the World Economic Forum [10].OOAC is a biomimetic system that can mimic the environment of a physiological organ, with the ability to regulate key parameters including AbstractThe organ-on-a-chip (OOAC) is in the list of top 10 emerging technologies and refers to a physiological organ biomimetic system built on a microfluidic chip. Through a combination of cell biology, engineering, and biomaterial technology, the microenvironment of the chip simulates that of the organ in terms of tissue interfaces and mechanical stimulation. This reflects the structural and functional characteristics of human tissue and can predict response to an array of stimuli including drug responses and environmental effects. OOAC has broad applications in precision medicine and biological defense strategies. Here, we introduce the concepts of OOAC and review its application to the construction of physiological models, drug development, and toxicology from the perspective of different organs. We further discuss existing challenges and provide future perspectives for its application.

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Molecular and functional resemblance of differentiated cells derived from isogenic human iPSCs and SCNT-derived ESCs

Cited by 66 publications

References 42 publications

Fibrostenotic eosinophilic esophagitis might reflect epithelial lysyl oxidase induction by fibroblast-derived TNF-α

Fibrostenotic eosinophilic esophagitis might reflect epithelial lysyl oxidase induction by fibroblast-derived TNF-α

Reprogramming mechanisms influence the maturation of hematopoietic progenitors from human pluripotent stem cells

Organ-on-a-chip: recent breakthroughs and future prospects

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