Molecular and functional explication of thioredoxin mitochondrial-like protein (Trx-2) from big-belly seahorse (Hippocampus abdominalis) and expression upon immune provocation

Nadarajapillai, Kishanthini; Sellaththurai, Sarithaa; Liyanage, D.S.; Yang, Hyerim; Lee, Jehee

doi:10.1016/j.fsi.2020.02.034

Cited by 10 publications

(3 citation statements)

References 34 publications

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“…Thioredoxins are essential components of the Tnx system characterized by a conserved Trp-Cys-Gly-Pro-Cys-Lys sequence at its active site ( Zhang et al, 2017 ). Structurally and functionally, Tnxs and Prdxs have been extensively studied in mammals and fish ( Zhang et al, 2017 ; Zhang et al, 2019 ; Nadarajapillai et al, 2020 ; Shahnaj et al, 2020 ) and their roles in diseases such as cancer, Alzheimer’s, heart attack and bacterial infections have been investigated. In reptiles, especially turtles, these important protein classes remain uncharacterized.…”

Section: Introductionmentioning

confidence: 99%

De novoassembly and functional annotation of blood transcriptome of loggerhead turtle, andin silicocharacterization of peroxiredoxins and thioredoxins

Fernández

Velasco

López-Barrera

et al. 2021

PeerJ

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The aim of this study was to generate and analyze the atlas of the loggerhead turtle blood transcriptome by RNA-seq, as well as identify and characterize thioredoxin (Tnxs) and peroxiredoxin (Prdxs) antioxidant enzymes of the greatest interest in the control of peroxide levels and other biological functions. The transcriptome of loggerhead turtle was sequenced using the Illumina Hiseq 2000 platform and de novo assembly was performed using the Trinity pipeline. The assembly comprised 515,597 contigs with an N50 of 2,631 bp. Contigs were analyzed with CD-Hit obtaining 374,545 unigenes, of which 165,676 had ORFs encoding putative proteins longer than 100 amino acids. A total of 52,147 (31.5%) of these transcripts had significant homology matches in at least one of the five databases used. From the enrichment of GO terms, 180 proteins with antioxidant activity were identified, among these 28 Prdxs and 50 putative Tnxs. The putative proteins of loggerhead turtles encoded by the genes Prdx1, Prdx3, Prdx5, Prdx6, Txn and Txnip were predicted and characterized in silico. When comparing Prdxs and Txns of loggerhead turtle with homologous human proteins, they showed 18 (9%), 52 (18%) 94 (43%), 36 (16%), 35 (33%) and 74 (19%) amino acid mutations respectively. However, they showed high conservation in active sites and structural motifs (98%), with few specific modifications. Of these, Prdx1, Prdx3, Prdx5, Prdx6, Txn and Txnip presented 0, 25, 18, three, six and two deleterious changes. This study provides a high quality blood transcriptome and functional annotation of loggerhead sea turtles.

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Section: Introductionmentioning

confidence: 99%

De novoassembly and functional annotation of blood transcriptome of loggerhead turtle, andin silicocharacterization of peroxiredoxins and thioredoxins

Fernández

Velasco

López-Barrera

et al. 2021

PeerJ

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“…As the co-enriched pathways of DEGs and SDMs identified in both pouch formation and pregnancy, aminoacyl-tRNA biosynthesis may participate in the formation of placenta-like tissues during male pregnancy by facilitating the growth of collagenous fibers. 40 Furthermore, antioxidant defense regulation, which is necessary to maintain a stable low concentration of reactive oxygen species, 41 plays a key role in male pregnancy ( Figure 4 ). Reproduction is an energetically demanding activity, and the metabolic rate of pregnant seahorses increases from 10% to 52% over pre-gravid levels.…”

Section: Discussionmentioning

confidence: 99%

Regulatory Role of Retinoic Acid in Male Pregnancy of the Seahorse

Qin

et al. 2020

The Innovation

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“…This system has thioredoxin reductase (Trx-R), reduced coenzyme II (NADPH), and thioredoxin (Trx). Thioredoxin acts as a redox carrier through disulfide bonds and thiols in a variety of reactions and is an important enzyme activity regulator protein [ 28 ]. The redox of disulfide bonds can efficiently transfer electrons and is the main regulation mechanisms in the signal.…”

Section: Oxidative Stressmentioning

confidence: 99%

Mitochondrial-Endoplasmic Reticulum Communication-Mediated Oxidative Stress and Autophagy

Liu

Hussain

Mehmood

et al. 2022

BioMed Research International

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Oxidative stress is an imbalance between free radicals and the antioxidant system causing overgeneration of free radicals (oxygen-containing molecules) ultimately leading to oxidative damage in terms of lipid peroxidation, protein denaturation, and DNA mutation. Oxidative stress can activate autophagy to alleviate oxidative damage and maintain normal physiological activities of cells by degrading damaged organelles or local cytoplasm. When oxidative stress is not eliminated by autophagy, it activates the apoptosis cascade. This review provides a brief summary of mitochondrial-endoplasmic reticulum communication-mediated oxidative stress and autophagy. Mitochondria and endoplasmic reticulum being important organelles in cells are directly or indirectly connected to each other through mitochondria-associated endoplasmic reticulum membranes and jointly regulate oxidative stress and autophagy. The reactive oxygen species (ROS) produced by the mitochondrial respiratory chain are the main inducers of oxidative stress. Damaged mitochondria can be effectively cleared by the process of mitophagy mediated by PINK1/parkin pathway, Nix/BNIP3 pathways, and FUNDC1 pathway, avoiding excessive ROS production. However, the mechanism of mitochondrial-endoplasmic reticulum communication in the regulation of oxidative stress and autophagy is rarely known. For this reason, this review explores the mutual connection of mitochondria and endoplasmic reticulum in mediating oxidative stress and autophagy through ROS and Ca 2+ and aims to provide part of the theoretical basis for alleviating oxidative stress through autophagy mediated by mitochondrial-endoplasmic reticulum communication.

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Molecular and functional explication of thioredoxin mitochondrial-like protein (Trx-2) from big-belly seahorse (Hippocampus abdominalis) and expression upon immune provocation

Cited by 10 publications

References 34 publications

De novoassembly and functional annotation of blood transcriptome of loggerhead turtle, andin silicocharacterization of peroxiredoxins and thioredoxins

De novoassembly and functional annotation of blood transcriptome of loggerhead turtle, andin silicocharacterization of peroxiredoxins and thioredoxins

Regulatory Role of Retinoic Acid in Male Pregnancy of the Seahorse

Mitochondrial-Endoplasmic Reticulum Communication-Mediated Oxidative Stress and Autophagy

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