Molecular and functional characterization of the barley yellow striate mosaic virus genes encoding phosphoprotein, P3, P6 and P9

Rabieifaradonbeh, Samira; Afsharifar, Alireza; Finetti-Sialer, M.

doi:10.1007/s10658-021-02307-7

Cited by 3 publications

(5 citation statements)

References 41 publications

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“…Furthermore, MPs may act in other important steps of the viral infection cycle. Currently, several studies have shown a role for MPs in suppressing the RNA silencing machinery [15,23,38,60,61]. A comprehensive study on the functionality of the dichorhaviruses MP suggests that this protein might guide the intracellular trafficking of the viral components and may be associated with the cell-to-cell movement mechanism of dichorhaviruses [33].…”

Section: Discussionmentioning

confidence: 99%

“…To unravel the mechanisms of suppression of RNA silencing by OFV MP, it would be interesting to investigate whether MP suppresses systemic PTGS, impairing the RNA-dependent RNA polymerase 6 (RDR6) or co-factors, as a suppressor of gene silencing 3 (SGS3), implicated in the genesis of secondary siRNA [65][66][67][68]. Suppression of the PTGS, impairing the RDR6/SGS3 pathway, has been reported for the P6 of RYSV, the P of ADV and LNYV, and the P and P3 (movement protein) of barley yellow striate mosaic cytorhabdovirus (BYSMV), a group of viruses evolutionary related to the dichorhaviruses [35,[37][38][39].…”

Section: Discussionmentioning

confidence: 99%

“…Dichorhaviruses share some similarities, including composition of structural proteins, gene order, moderate to high sequence similarity, and nuclear localization, with non-segmented plant rhabdoviruses, especially with the alpha, beta and gamma nucleorhabdoviruses [27,31,34]. For rhabdoviruses, conserved structural protein (P) and accessory gene-encoded proteins (P6 and P3) have been identified as acting as VSRs by interacting with components of the local and/or systemic RNA silencing machinery [35][36][37][38][39]. Although current studies have advanced the understanding of the molecular processes associated with the suppressive activity of proteins encoded by rhabdoviruses, this same progress has not been reported for dichorhaviruses, which are evolutionarily close to this group.…”

Section: Introductionmentioning

confidence: 99%

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Orchid fleck dichorhavirus movement protein shows RNA silencing suppressor activity

Leastro

Pallás

Sánchez‐Navarro

2022

Journal of General Virology

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To counteract RNA interference-mediated antiviral defence, virus genomes evolved to express proteins that inhibit this plant defence mechanism. Using six independent biological approaches, we show that orchid fleck dichorhavirus citrus strain (OFV-citrus) movement protein (MP) may act as a viral suppressor of RNA silencing (VSR). By using the alfalfa mosaic virus (AMV) RNA 3 expression vector, it was observed that the MP triggered necrosis response in transgenic tobacco leaves and increased the viral RNA (vRNA) accumulation. The use of the potato virus X (PVX) expression system revealed that the cis expression of MP increased both the severity of the PVX infection and the accumulation of PVX RNAs, further supporting that MP could act as an RNA silencing suppressor (RSS). From the analysis of the RSS-defective turnip crinkle virus (TCV), we do not find local RSS activity for MP, suggesting a link between MP suppressor activity and the prevention of systemic silencing. In the analysis of local suppressive activity using the GFP-based agroinfiltration assay in Nicotiana benthamiana (16 c line), we do not identify local RSS activity for the five OFV RNA1-encoded proteins. However, when evaluating the small interfering RNA (siRNA) accumulation, we find that the expression of MP significantly reduces the accumulation of GFP-derived siRNA. Finally, we examine whether the MP can prevent systemic silencing in 16c plants. Our findings show that MP inhibits the long-distance spread of RNA silencing, but does not affect the short-distance spread. Together, our findings indicate that MP is part of OFV’s counter-defence mechanism, acting mainly in the prevention of systemic long-distance silencing. This work presents the first report of a VSR for a member of the genus Dichorhavirus.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Orchid fleck dichorhavirus movement protein shows RNA silencing suppressor activity

Leastro

Pallás

Sánchez‐Navarro

2022

Journal of General Virology

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“…In contrast to members of the genus Dichorhavirus, the structural (virion) P protein of monopartite plant rhabdoviruses has been shown to have a silencing suppressor function [173][174][175][176]. The P protein interacts with AGO proteins, RDR6 and SGS3; accordingly, the P protein has been shown to inhibit the activity of AGO1-containing RISC complexes and the amplification of RNA silencing by interfering with the synthesis of secondary siRNAs [174,175].…”

Section: Order Mononegaviralesmentioning

confidence: 99%

“…Other rhabdovirus proteins have also been shown to be silencing suppressors. In Barley yellow striate mosaic virus, the structural P3 protein has been shown to have silencing suppressor activity in addition to the P protein [176], whereas in the Rice yellow stunt virus the only identified suppressor is the virion P6 protein [177].…”

Section: Order Mononegaviralesmentioning

confidence: 99%

Role of Plant Virus Movement Proteins in Suppression of Host RNAi Defense

Atabekova

Solovieva

Chergintsev

et al. 2023

IJMS

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One of the systems of plant defense against viral infection is RNA silencing, or RNA interference (RNAi), in which small RNAs derived from viral genomic RNAs and/or mRNAs serve as guides to target an Argonaute nuclease (AGO) to virus-specific RNAs. Complementary base pairing between the small interfering RNA incorporated into the AGO-based protein complex and viral RNA results in the target cleavage or translational repression. As a counter-defensive strategy, viruses have evolved to acquire viral silencing suppressors (VSRs) to inhibit the host plant RNAi pathway. Plant virus VSR proteins use multiple mechanisms to inhibit silencing. VSRs are often multifunctional proteins that perform additional functions in the virus infection cycle, particularly, cell-to-cell movement, genome encapsidation, or replication. This paper summarizes the available data on the proteins with dual VSR/movement protein activity used by plant viruses of nine orders to override the protective silencing response and reviews the different molecular mechanisms employed by these proteins to suppress RNAi.

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Advancements in Imaging Sensors and AI for Plant Stress Detection: A Systematic Literature Review

Walsh,

Mangina,

Negrão

2024

Plant Phenomics

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Integrating imaging sensors and artificial intelligence (AI) have contributed to detecting plant stress symptoms, yet data analysis remains a key challenge. Data challenges include standardized data collection, analysis protocols, selection of imaging sensors and AI algorithms, and finally, data sharing. Here, we present a systematic literature review (SLR) scrutinizing plant imaging and AI for identifying stress responses. We performed a scoping review using specific keywords, namely abiotic and biotic stress, machine learning, plant imaging and deep learning. Next, we used programmable bots to retrieve relevant papers published since 2006. In total, 2,704 papers from 4 databases (Springer, ScienceDirect, PubMed, and Web of Science) were found, accomplished by using a second layer of keywords (e.g., hyperspectral imaging and supervised learning). To bypass the limitations of search engines, we selected OneSearch to unify keywords. We carefully reviewed 262 studies, summarizing key trends in AI algorithms and imaging sensors. We demonstrated that the increased availability of open-source imaging repositories such as PlantVillage or Kaggle has strongly contributed to a widespread shift to deep learning, requiring large datasets to train in stress symptom interpretation. Our review presents current trends in AI-applied algorithms to develop effective methods for plant stress detection using image-based phenotyping. For example, regression algorithms have seen substantial use since 2021. Ultimately, we offer an overview of the course ahead for AI and imaging technologies to predict stress responses. Altogether, this SLR highlights the potential of AI imaging in both biotic and abiotic stress detection to overcome challenges in plant data analysis.

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Molecular and functional characterization of the barley yellow striate mosaic virus genes encoding phosphoprotein, P3, P6 and P9

Cited by 3 publications

References 41 publications

Orchid fleck dichorhavirus movement protein shows RNA silencing suppressor activity

Orchid fleck dichorhavirus movement protein shows RNA silencing suppressor activity

Role of Plant Virus Movement Proteins in Suppression of Host RNAi Defense

Advancements in Imaging Sensors and AI for Plant Stress Detection: A Systematic Literature Review

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