Molecular and functional characterization of voltage-gated sodium channels in human sperm

Pinto, Francisco M.; Ravina, Cristina G.; Sánchez, Manuel Fernández; Gallardo-Castro, Manuel; Cejudo-Román, Antonio; Candenas, Luz

doi:10.1186/1477-7827-7-71

Cited by 34 publications

(58 citation statements)

References 36 publications

(70 reference statements)

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“…None of these genes are listed as affecting either sperm axonemal structure or sperm motility (21) or as participating in spermatozoa maturation, motility, or fertilization in a 2008 review (22). Several of these genes (Skiv2l2, Scn10a, and Egr1) have subsequently been reported to be relevant to spermatogenesis and reproductive function (23)(24)(25), but none have been shown to be involved with axoneme formation or to result in the phenotype seen in the Stamp tm/tm mice following disruption/overexpression (26,27). Interestingly, 6 of the 33 affected genes are known to be regulated by androgen receptors.…”

Section: Absence Of Stamp Protein Does Not Alter Expression Of Genes mentioning

confidence: 99%

Disruption of Ttll5/Stamp Gene (Tubulin Tyrosine Ligase-like Protein 5/SRC-1 and TIF2-associated Modulatory Protein Gene) in Male Mice Causes Sperm Malformation and Infertility

Lee

He²,

Dougherty³

et al. 2013

Journal of Biological Chemistry

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Background: Ttll5/STAMP is a multifunctional protein in cells with unknown activity in animals. Results: Targeted disruption of the Ttll5/Stamp gene in mice causes male infertility with reduced ␣-tubulin polyglutamylation and axoneme disruption in sperm. Conclusion: Ttll5/Stamp deficiency differs from previously described defects in sperm maturation and function. Significance: Ttll5/Stamp is a new gene involved in sperm maturation that may be relevant for human fertility.

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Section: Absence Of Stamp Protein Does Not Alter Expression Of Genes mentioning

confidence: 99%

Disruption of Ttll5/Stamp Gene (Tubulin Tyrosine Ligase-like Protein 5/SRC-1 and TIF2-associated Modulatory Protein Gene) in Male Mice Causes Sperm Malformation and Infertility

Lee

He²,

Dougherty³

et al. 2013

Journal of Biological Chemistry

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“…The sample was centrifuged at 200 g for 10 min, and then resuspended in DPBS. Two excitation wavelengths (340 and 380 nm) were used and emitted fluorescence was detected at 510 nm (Pinto et al, 2009). [Ca 2+ ] i was calculated as the ratio of the fluorescence at 340 to that at 380 nm (F340/F380).…”

Section: Detection Of Intracellular Calcium Ion Concentration ([Ca 2+mentioning

confidence: 99%

Sodium nitroprusside suppresses male fertility in vitro

et al. 2014

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SUMMARYSodium nitroprusside is a nitric oxide donor involved in the regulation of the motility, hyperactivation, capacitation, and acrosome reaction (AR) of spermatozoa. However, the molecular mechanism underlying this regulation has not yet been elucidated. Therefore, this study was designed to evaluate the molecular basis for the effects of sodium nitroprusside on different processes in spermatozoa and its consequences on subsequent oocyte fertilization and embryo development. In this in vitro study, mouse spermatozoa were incubated with various concentrations of sodium nitroprusside (1, 10, and 100 lM) for 90 min. Our results showed that sodium nitroprusside inhibited sperm motility and motion kinematics in a dose-dependent manner by significantly enhancing intracellular iron and reactive oxygen species (ROS), and decreasing Ca 2+ , and adenosine triphosphate levels in spermatozoa. Moreover, short-term exposure of spermatozoa to sodium nitroprusside increased the tyrosine phosphorylation of sperm proteins involved in PKA-dependent regulation of intracellular calcium levels, which induced a robust AR. Finally, sodium nitroprusside significantly decreased the rates of fertilization and blastocyst formation during embryo development. Based on these results, we propose that sodium nitroprusside increases ROS production and precocious AR may alter overall sperm physiology, leading to poor fertilization and compromised embryonic development.

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“…The presence of voltage-gated Na + channels (Na V ) has been documented in human sperm by mRNA expression (Na V 1.1-1.9), proteomics (see Table 3.3), and immunofluorescence (all except Na V 1.1 and 1.3) (Pinto et al 2009). Though the role of these channels in sperm motility was investigated, their exact function remains poorly understood.…”

Section: Na + Channelsmentioning

confidence: 99%

Role of Ion Channels in the Sperm Acrosome Reaction

Beltrán

Treviño

Mata-Martínez

et al. 2016

Sperm Acrosome Biogenesis and Function During Fertilization

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The acrosome reaction (AR) is a unique exocytotic process where the acrosome, a single membrane-delimited specialized organelle, overlying the nucleus in the sperm head of many species, fuses with the overlying plasma membrane. This reaction, triggered by physiological inducers from the female gamete, its vicinity, or other stimuli, discharges the acrosomal content modifying the plasma membrane, incorporating the inner acrosomal membrane, and exposing it to the extracellular medium. The AR is essential for sperm-egg coat penetration, fusion with the eggs' plasma membrane, and fertilization. As in most exocytotic processes Ca(2+) is crucial for the AR, as well as intracellular pH and membrane potential changes. Thus, among the required processes needed for this reaction, ion permeability changes involving channels are pivotal. In spite of the key role ion channels play in the AR, their identity and regulation is not fully understood. Though molecular and pharmacological evidence indicates that various ionic channels participate during the AR, such as store-operated Ca(2+) channels and voltage-dependent Ca(2+) channels, whole cell patch clamp recordings have failed to detect some of them until now. Since sperm display a very high resistance and a minute cytoplasmic volume, very few channels are needed to achieve large membrane potential and concentration changes. Functional detection of few channels in the morphologically complex and tiny sperm poses technical problems, especially when their conductance is very small, as in the case of SOCs. Single channel recordings and novel fluorescence microscopy strategies will help to define the participation of ionic channels in the intertwined signaling network that orchestrates the AR.

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Molecular and functional characterization of voltage-gated sodium channels in human sperm

Abstract: Background: We have investigated the expression of voltage-gated sodium channels in human spermatozoa and characterized their role in sperm motility.

Cited by 34 publications

References 36 publications

Disruption of Ttll5/Stamp Gene (Tubulin Tyrosine Ligase-like Protein 5/SRC-1 and TIF2-associated Modulatory Protein Gene) in Male Mice Causes Sperm Malformation and Infertility

Disruption of Ttll5/Stamp Gene (Tubulin Tyrosine Ligase-like Protein 5/SRC-1 and TIF2-associated Modulatory Protein Gene) in Male Mice Causes Sperm Malformation and Infertility

Sodium nitroprusside suppresses male fertility in vitro

Role of Ion Channels in the Sperm Acrosome Reaction

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