Molecular and functional characterization of the magnesium transporter gene ZmMGT12 in maize

Li, Hongyou; Lee, Chan; Zhou, Lina; Zhao, Zhuo; Li, Yihong; Qu, Min; Kai-feng, Huang; Zhang, Lu; Lu, Yanli; Cao, Moju; Gao, Suping; Zhang, Suzhi

doi:10.1016/j.gene.2018.04.068

Cited by 22 publications

(15 citation statements)

References 38 publications

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“…The transporter genes like Al, Mg, and K transporter are reporting for the first time in pearl millet. Whereas these transporter gene family were found previously in many different crops such as Al transporters gene in wheat ( Li T. et al, 2017 ) and Rice ( Sonoda et al, 2003 ); Mg transporter gene in Maize ( Li et al, 2016 , 2018 ) and Arabidopsis ( Li H. et al, 2017 ); K transporter gene in Maize ( Zhang et al, 2012 ) and Barley ( Santa-María et al, 1997 ); and Zn transporter gene in Arabidopsis ( Grotz et al, 1998 ), rice ( Ishimaru et al, 2005 ; Lee et al, 2010 ; Neeraja et al, 2018 ) and Maize ( Li et al, 2013 ).…”

Section: Validation Of Identified Qtls In a Diverse Set Of Germplasmmentioning

confidence: 70%

Multi-Environment Quantitative Trait Loci Mapping for Grain Iron and Zinc Content Using Bi-parental Recombinant Inbred Line Mapping Population in Pearl Millet

Singhal

Satyavathi²,

Singh

et al. 2021

Front. Plant Sci.

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Pearl millet is a climate-resilient, nutritious crop with low input requirements that could provide economic returns in marginal agro-ecologies. In this study, we report quantitative trait loci (QTLs) for iron (Fe) and zinc (Zn) content from three distinct production environments. We generated a genetic linkage map using 210 F6 recombinant inbred line (RIL) population derived from the (PPMI 683 × PPMI 627) cross using genome-wide simple sequence repeats (SSRs). The molecular linkage map (seven linkage groups) of 151 loci was 3,273.1 cM length (Kosambi). The content of grain Fe in the RIL population ranged between 36 and 114 mg/Kg, and that of Zn from 20 to 106 mg/Kg across the 3 years (2014–2016) at over the three locations (Delhi, Dharwad, and Jodhpur). QTL analysis revealed a total of 22 QTLs for grain Fe and Zn, of which 14 were for Fe and eight were for Zn on three consecutive years at all locations. The observed phenotypic variance (R2) explained by different QTLs for grain Fe and Zn content ranged from 2.85 (QGFe.E3.2014–2016_Q3) to 19.66% (QGFe.E1.2014–2016_Q3) and from 2.93 (QGZn.E3.2014–2016_Q3) to 25. 95% (QGZn.E1.2014–2016_Q1), respectively. Two constitutive expressing QTLs for both Fe and Zn co-mapped in this population, one on LG 2 and second one on LG 3. Inside the QTLs candidate genes such as Ferritin gene, Al3+ Transporter, K+ Transporters, Zn2+ transporters and Mg2+ transporters were identified using bioinformatics approaches. The identified QTLs and candidate genes could be useful in pearl millet population improvement programs, seed, restorer parents, and marker-assisted selection programs.

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Section: Validation Of Identified Qtls In a Diverse Set Of Germplasmmentioning

confidence: 70%

Multi-Environment Quantitative Trait Loci Mapping for Grain Iron and Zinc Content Using Bi-parental Recombinant Inbred Line Mapping Population in Pearl Millet

Singhal

Satyavathi²,

Singh

et al. 2021

Front. Plant Sci.

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Section: Discussionmentioning

confidence: 98%

“…Some genes seem to play a universal role. For example, MaMRS2-6 was highly expressed in leaves of banana seedlings ( Fig 7 ), and its counterpart, which was deemed to be involved in photosynthesis in maize, Arabidopsis and rice, was also highly expressed in leaves [ 18 , 25 , 34 ]. Similarly, banana MaMRS2-4 gene was significantly up-regulated in the banana roots under Mg-deficiency ( Fig 7 ), MaMRS2-4 is homologous to AtMRS2-4/AtMGT6 in Arabidopsis, ZmMGT10 in maize and SlMGT4-1 in tomato, whose respective up-regulation was observed in the roots when deprived of Mg [ 22 , 33 , 35 , 36 ].…”

Section: Discussionmentioning

confidence: 99%

“…Similarly, rice OsMRS2-5 is expressed in tissues other than flag leaves, and OsMRS2-8 is expressed in all tissues but rarely in leaves [ 18 ], and pear PbrMGT4 is expressed only in leaves while PbrMGT16 is expressed solely in pistils [ 21 ]. In addition, the expression of MGTs in plants is also affected by circadian rhythm and light [ 18 , 20 , 25 ]. The versatile Mg transporter genes with diverse expression pattern in plants contribute to their functional complexity.…”

Section: Introductionmentioning

confidence: 99%

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Identification and functional analysis of the CorA/MGT/MRS2-type magnesium transporter in banana

Tong

Wen

et al. 2020

PLoS ONE

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Magnesium (Mg) plays an irreplaceable role in plant growth and development. Mg transporters, especially CorA/MGT/MRS2 family proteins, played a vital role in regulating Mg content in plant cells. Although extensive work has been conducted in model crops, such as Arabidopsis, rice, and maize, the relevant information is scarce in tropical crops. In this study, 10 MaMRS2 genes in banana (Musa acuminata) were isolated from its genome and classified into five distinct clades. The putative physiochemical properties, chromosome location, gene structure, cis-acting elements, and duplication relationships in between these members were analyzed. Complementary experiments revealed that three MaMRS2 gene members (MaMRS2-1, MaMRS2-4, MaMRS2-7), from three distinct phylogenetic branches, were capable of restoring the function of Mg transport in Salmonella typhimurium mutants. Semi-quantitative RT-PCR showed that MaMRS2 genes were differentially expressed in banana cultivar 'Baxijiao' (Musa spp. AAA Cavendish) seedlings. The result was confirmed by real-time PCR analysis, in addition to tissue specific expression, expression differences among MaMRS2 members were also observed under Mg deficiency conditions. These results showed that Mg transporters may play a versatile role in banana growth and development, and our work will shed light on the functional analysis of Mg transporters in banana.

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“…The gene magnesium transporter 2 (mgt2) is found in proximity of the association on chromosome 6, position 98.450, affecting DMC in C. The gene mgt2 controls partitioning of magnesium in developing tissues, and magnesium loading in shoot effects on plant growth are manifold. Growth maintenance requires magnesium translocation [40] first for the utilization in chlorophyll synthesis [41,42], and second for maintenance of osmotic balance, as Mg 2+ ions are osmotically highly active. Control of vacuolar magnesium contents in developing tissues is a highly important process for maintenance of plant growth affected by many cell-specific processes [43].…”

Section: Allelic Effects and Candidate Genesmentioning

confidence: 99%

Seed Weight as a Covariate in Association and Prediction Studies for Biomass Traits in Maize Seedlings

Galić

Mazur

Brkić

et al. 2020

Plants

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Background: The seedling stage has received little attention in maize breeding to identify genotypes tolerant to water deficit. The aim of this study is to evaluate incorporation of seed weight (expressed as hundred kernel weight, HKW) as a covariate into genomic association and prediction studies for three biomass traits in a panel of elite inbred lines challenged by water withholding at seedling stage. Methods: 109 genotyped-by-sequencing (GBS) elite maize inbreds were phenotyped for HKW and planted in controlled conditions (16/8 day/night, 25 °C, 50% RH, 200 µMol/m2/s) in trays filled with soil. Plants in control (C) were watered every two days, while watering was stopped for 10 days in water withholding (WW). Fresh weight (FW), dry weight (DW), and dry matter content (DMC) were measured. Results: Adding HKW as a covariate increased the power of detection of associations in FW and DW by 44% and increased genomic prediction accuracy in C and decreased in WW. Conclusions: Seed weight was effectively incorporated into association studies for biomass traits in maize seedlings, whereas the incorporation into genomic predictions, particularly in water-stressed plants, was not worthwhile.

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Molecular and functional characterization of the magnesium transporter gene ZmMGT12 in maize

Cited by 22 publications

References 38 publications

Multi-Environment Quantitative Trait Loci Mapping for Grain Iron and Zinc Content Using Bi-parental Recombinant Inbred Line Mapping Population in Pearl Millet

Multi-Environment Quantitative Trait Loci Mapping for Grain Iron and Zinc Content Using Bi-parental Recombinant Inbred Line Mapping Population in Pearl Millet

Identification and functional analysis of the CorA/MGT/MRS2-type magnesium transporter in banana

Seed Weight as a Covariate in Association and Prediction Studies for Biomass Traits in Maize Seedlings

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