Molecular and functional characterisation of the zebrafish (Danio rerio) PEPT1‐type peptide transporter1

Verri, Tiziano; Kottra, Gábor; Romano, Alessandro; Tiso, Natascia; Perić, Mark; Maffia, Michele; Boll, Michael; Argenton, Francesco; Daniel, Hannelore; Storelli, Carlo

doi:10.1016/s0014-5793(03)00759-2

Cited by 139 publications

(145 citation statements)

References 30 publications

(48 reference statements)

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“…The predicted protein, designated as codPepT1, shares a high overall identity with PepT1-type transporters (58-63%) when compared to other known PepT1 proteins in vertebrates (Fig.·2), and clusters to the 'fish' branch of the reconstructed phylogenetic tree, together with zebrafish PepT1 (Fig.·3). To date, codPepT1 represents the second PepT1-type peptide transporter recognized from a teleost fish [after zebrafish PepT1 (Verri et al, 2003)], thus representing an additional molecular tool for the understanding of evolutionary and functional relationships among vertebrate peptide transporters.…”

Section: Discussionmentioning

confidence: 99%

“…Phylogenetic trees were constructed with bootstrap confidence values based on 1000 replicates. GenBank accession numbers for amino acid sequence comparisons are: AAQ65244 [zebrafish PEPT1 (Verri et al, 2003)], AAA17721 [rabbit PEPT1 (Fei et al, 1994)], NP_001003036 (dog PEPT1), AAO43094 [pig PEPT1 (Klang et al, 2005)], XP_599441 (bovine PEPT1), AAB61693 [human PEPT1 (Liang et al, 1995)], NP_001028071 [macaque PEPT1 (Zhang et al, 2004a)], BAA09318 [rat PEPT1 (Miyamoto et al, 1996)], NP_444309 [mouse PEPT1 (Fei et al, 2000)], AAK14788 [sheep PEPT1 (Pan et al, 2001)], AAK39954 [chicken PEPT1 (Chen et al, 2002)], AAO16604 (turkey PEPT1).…”

Section: In Silico Analysismentioning

confidence: 99%

“…Like zebrafish PepT1 (Verri et al, 2003), it is evolutionarily distant from the sequences already characterized from higher vertebrates, i.e. birds and mammals (for a review, see Daniel et al, 2006), and thus represents a novel reference sequence to support comparative analysis along the vertebrate series.…”

Section: Fish Vs Tetrapod Pept1 Proteins: a Comparisonmentioning

confidence: 99%

“…In contrast, information about these proteins in lower vertebrates is very limited, with the sole exception of the PepT1-type oligopeptide transporter of the teleost zebrafish Danio rerio, whose functional activity and pattern of expression in tissue has been assessed (Verri et al, 2003). In particular, zebrafish PepT1 is highly expressed in the proximal intestine, where it mediates the uptake of a large amount of di-and tripeptides derived from the protein digestion process.…”

Section: Introductionmentioning

confidence: 99%

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Oligopeptide transporter PepT1 in Atlantic cod (Gadus morhuaL.): cloning, tissue expression and comparative aspects

Rønnestad

Gavaia

Viegas

et al. 2007

Journal of Experimental Biology

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A novel full-length cDNA that encodes for the Atlantic cod (Gadus morhua L.) PepT1-type oligopeptide transporter has been cloned. This cDNA (named codPepT1) was 2838·bp long, with an open reading frame of 2190·bp encoding a putative protein of 729 amino acids. Comparison of the predicted Atlantic cod PepT1 protein with zebrafish, bird and mammalian orthologs allowed detection of many structural features that are highly conserved among all the vertebrate proteins analysed, including (1) a larger than expected area of hydrophobic amino acids in close proximity to the N terminus; (2) a single highly conserved cAMP/cGMP-dependent protein kinase phosphorylation motif; (3) a large N-glycosylationrich region within the large extracellular loop; and (4) a conserved and previously undescribed stretch of 8-12 amino acid residues within the large extracellular loop. Expression analysis at the mRNA level indicated that Atlantic cod PepT1 is mainly expressed at intestinal level, but that it is also present in kidney and spleen. Analysis of its regional distribution along the intestinal tract of the fish revealed that PepT1 is ubiquitously expressed in all segments beyond the stomach, including the pyloric caeca, and through the whole midgut. Only in the last segment, which included the hindgut, was there a lower expression. Atlantic cod PepT1, the second teleost fish PepT1-type transporter documented to date, will contribute to the elucidation of the evolutionary and functional relationships among vertebrate peptide transporters. Moreover, it can represent a useful tool for the study of gut functional regionalization, as well as a marker for the analysis of temporal and spatial expression during ontogeny.

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Section: Discussionmentioning

confidence: 99%

Section: In Silico Analysismentioning

confidence: 99%

Section: Fish Vs Tetrapod Pept1 Proteins: a Comparisonmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Oligopeptide transporter PepT1 in Atlantic cod (Gadus morhuaL.): cloning, tissue expression and comparative aspects

Rønnestad

Gavaia

Viegas

et al. 2007

Journal of Experimental Biology

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“…Pairwise and multiple sequence alignments were carried out using the ClustalW program (http://www.ebi.ac.uk/clustalw). GenBank accession numbers for sequence comparisons were: human, Homo sapiens PepT1 (AAB61693; Liang et al 1995), rabbit, Oryctolagus cuniculus PepT1 (AAA17721; Fei et al 1994), rat, Rattus norvegicus PepT1 (BAA09318; Miyamoto et al 1996), mouse, Mus musculus PepT1 (AAF81666; Fei et al 2000), sheep, Ovis aries PepT1 (AAK14788; Pan et al 2001), pig, Sus scrofa PepT1 (AAO43094; Klang et al 2005), dog, Canis lupus familiaris PepT1 (AAL67837; MadinDarby canine kidney cell), chicken, Gallus gallus PepT1 (AAK39954; Chen et al 2002), turkey, Meleagris gallopavo PepT1 (AAO16604; Van et al 2005), zebrafish, Danio rerio PepT1 (AAQ65244; Verri et al 2003), icefish, Chionodraco hamatus PepT1 (AAO39705; , and Atlantic cod, Gadus morhua PepT1 (AY921634; Rønnestad et al 2007). …”

Section: Methodsmentioning

confidence: 99%

Cloning Two PepT1 cDNA Fragments of Common Carp, Cyprinus Carpio (Actinopterygii: Cypriniformes: Cyprinidae)

Ostaszewska¹,

Szatkowska²,

Verri³

et al. 2009

Acta Icth et Piscat

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Background. Common carp, Cyprinus carpio, is a model organism within Teleostei. Oligopeptides are a new and promising alternative source of amino acids in animal as well as in human nutrition. In common carp, the membrane protein that transports oligopeptides across the enterocyte membrane is encoded by the gene PepT1 (SLC15A1). The aim of this paper was to sequence the PepT1 (SLC15A1) in common carp. Materials and Methods. Intestine samples were isolated from six-week old common carp. Total RNA was isolated using a Trizol method. Reverse transcription was used to synthesize cDNA. Two different pairs of primers were designed, according to the zebrafish (Danio rerio) PepT1 sequence, and used for PCR. The amplified DNA was isolated by electrophoresis, cloned (pCRII-TOPO vectors), sequenced, and subjected to in silico analysis. Results. Two nucleotide fragments of the PepT1 gene were obtained and analyzed using bioinformatic tools. Both fragments showed a high degree of homology with the known PepT1 genes of other teleosts, mammals, and birds. High homology of the PepT1 gene, and similar primary protein structure among the aforementioned taxa probably reflects the conservative function of the PepT1 protein product. Both fragments of the PepT1 gene were deposited in GenBank (FJ556590; FJ529670). Conclusions. The sequenced fragments of the common carp PepT1 gene will allow evaluation of PepT1 expression in the intestines of fish fed diets containing various forms of protein, which is an issue of importance regarding fish nutrition and its import for aquaculture.

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Proteins

Conceição¹,

Aragão²,

Rønnestad³

2011

Larval Fish Nutrition

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Molecular and functional characterisation of the zebrafish (Danio rerio) PEPT1‐type peptide transporter¹

Cited by 139 publications

References 30 publications

Oligopeptide transporter PepT1 in Atlantic cod (Gadus morhuaL.): cloning, tissue expression and comparative aspects

Oligopeptide transporter PepT1 in Atlantic cod (Gadus morhuaL.): cloning, tissue expression and comparative aspects

Cloning Two PepT1 cDNA Fragments of Common Carp, <I>Cyprinus Carpio</I> (Actinopterygii: Cypriniformes: Cyprinidae)

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