Molecular and expression characterization of two somatostatin genes in the Chinese sturgeon, Acipenser sinensis

Li, Chuangju; Wei, Qiwei; Zhou, Li; Cao, Hong; Yan, Zhang; Gui, Jian‐Fang

doi:10.1016/j.cbpa.2009.05.012

Cited by 15 publications

(12 citation statements)

References 44 publications

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“…For example, multiple somatostatin and somatostatin receptor genes were characterized in the Chinese sturgeon (Acipenser sinensis) [95] and in groupers [96][97][98][99]. Orangespotted grouper orexin was found to be involved in feeding and orexin-A was a stimulator of NPY mRNA expression in vivo and in vitro [100].…”

Section: Growth Trait and Candidate Growth-related Genesmentioning

confidence: 99%

Molecular basis and genetic improvement of economically important traits in aquaculture animals

2012

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Aquaculture has been believed to be a major Chinese contribution to the world. In recent 20 years, genome and other genetic technologies have promoted significant advances in basic studies on molecular basis and genetic improvement of aquaculture animals, and complete genomes of some main aquaculture animals have been sequenced or announced to be sequenced since the beginning of this century. Here, we review some significant breakthrough progress of aquaculture genetic improvement technologies including genome technologies, somatic cell nuclear transfer and stem cell technologies, outline the molecular basis of several economically important traits including reproduction, sex, growth, disease resistance, cold tolerance and hypoxia tolerance, and present a series of candidate trait-related genes. Finally, some application cases of genetic improvement are introduced in aquaculture animals, especially in China, and several development trends are highlighted in the near future.

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Section: Growth Trait and Candidate Growth-related Genesmentioning

confidence: 99%

Molecular basis and genetic improvement of economically important traits in aquaculture animals

2012

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“…1-year-old individuals were a female and a male; 2-and 3-year-old were male; 4-and 5-year-old were female (data not shown). The average length and weight of individuals in each age group were 0.60 m and 0.92 kg, 1.02 m and 5.25 kg, 1.22 m and 8.4 kg, 1.26 m and 8.3 kg, 1.30 m and 11.9 kg, respectively (Li et al 2009). The experimental procedures were based on the standards of the Chinese Council on Animal Care.…”

Section: Animals and Samplesmentioning

confidence: 93%

“…This task is rather difficult, because so far little is known about regulation mechanism of growth and reproduction in the Chinese sturgeon. Recently, we have established a systematic molecular study in the Chinese sturgeon, and identified some important genes related to the growth, development, and reproduction, such as somatostatin 1 and 2 (Li et al 2009), three gonadotropin subunits common a, FSHb and LHb , three ZP protein genes (Li et al 2011a, b), apolipoprotein E (Li et al 2011a, b), growth hormone (GH) (Cao et al 2011), thyroid-stimulating hormone subunit b (TSHb), and insulin-like growth factor I (IGF-I) (in preparation). In order to protect this species, germ cell transplantation is believed to be an available method.…”

Section: Introductionmentioning

confidence: 99%

Identification of a pou2 ortholog in Chinese sturgeon, Acipenser sinensis and its expression patterns in tissues, immature individuals and during embryogenesis

Chen

et al. 2011

Fish Physiol Biochem

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The class V POU family genes, including pou5f1 and pou2, encode transcription factors critical for the maintenance of pluripotency in embryonic stem cells (ESC) and germ line cells in vertebrates. In the present study, the full-length cDNA of a pou2 ortholog in A. sinensis, Aspou2, was cloned and sequenced. This cDNA sequence is 2,853 base pairs in length and encodes a peptide of 431 amino acid residues. A comparison of the deduced amino acid sequence of Aspou2 with that of other vertebrate species showed that they were highly conserved in the POU domain, which shared 88 and 90% identity with that of zebrafish and medaka, respectively, and was 69, 67 and 67% identical to frog, mouse and human, respectively. RT-PCR analysis revealed that Aspou2 was detected in all tissues examined except for the liver, and high mRNA levels of Aspou2 were found in the muscle, pituitary and brain. During the embryogenesis and early larval development, the expression level of Aspou2 mRNAs decreased gradually apart from 1-day larvae that were not observed. Furthermore, Aspou2 seemed to raise with the development of gonads of immature Chinese sturgeons. These results suggested the possible involvement of Aspou2 in the nonpluripotent cells, pluripotent cells, embryogenesis, and gonad development.

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“…Therefore, understanding of its growth and reproduction regulation should start from the early stages. Recently, we have initiated a systematic study in molecular biology of the Chinese sturgeon, and identified some important genes involved in growth and reproduction, such as three gonadotropin subunits common a, FSHb and LHb [3], and two somatostatins [4].…”

Section: Introductionmentioning

confidence: 99%

EST dataset of pituitary and identification of somatolactin and novel genes in Chinese sturgeon, Acipenser sinensis

Cao

Leng

et al. 2011

Mol Biol Rep

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Chinese sturgeon (Acipenser sinensis) is a rare and endangered species and also an important resource for the sturgeon aquaculture industry, however, a few genes have been identified in this species. We report here construction of a pituitary cDNA library from a 24 years old female Chinese sturgeon just after its spawning, and obtained 2,025 ESTs from the library. 885 unique sequences were identified, which were categorized into 12 functional groups. More than half of the unique sequences (57%) do not match with annotated sequences in the public databases. Three of these novel genes were further identified. Notably, a full-length of cDNA (1,143 bp) encoding somatolactin of 232 amino acids was identified. Phylogenetic analysis showed 97% amino acid identity with White sturgeon somatolactin. RT-PCR analysis indicated that the somatolactin mRNA was only detected in pituitary. Pituitary-specific expression of the somatolactin suggested that the protein may play important physiological functions in pituitary-endocrine system of the Chinese sturgeon.

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Molecular and expression characterization of two somatostatin genes in the Chinese sturgeon, Acipenser sinensis

Cited by 15 publications

References 44 publications

Molecular basis and genetic improvement of economically important traits in aquaculture animals

Molecular basis and genetic improvement of economically important traits in aquaculture animals

Identification of a pou2 ortholog in Chinese sturgeon, Acipenser sinensis and its expression patterns in tissues, immature individuals and during embryogenesis

EST dataset of pituitary and identification of somatolactin and novel genes in Chinese sturgeon, Acipenser sinensis

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