Molecular and epidemiological analysis of penicillinase producing strains of Neisseria gonorrhoeae isolated in Canada 1976-84: evolution of new auxotypes and beta lactamase encoding plasmids.

Beta-lactamase plasmids and chromosomally mediated antibiotic resistance in pathogenic Neisseria species

“…been extended in N. gonorrhoeae from original observations to include other auxotypes; the transfer plasmid has subsequently become established in isolates with the 5.1-kb plasmid (2,22,75).…”

Auxotypes, plasmid contents, and serovars of gonococcal strains (PPNG and non-PPNG) from Jamaica.

“…We assessed nutritional growth requirements (auxotype) using the method of Hendry and Stewart22 as modified by Hendry.23 Plasmid content was analysed using the method published by Dillon et al' Control strains for plasmid content analysis and auxotyping have been described previously. 5 Serological characterisation of 168 non-PPNG strains and 20 PPNG strains by coagglutination using monoclonal antibodies to protein I was performed using methods described by Tam et al9 and Bygdeman et al 24 Two panels of monoclonal reagents were used, the GS and Ph reagents.25 The designation of specific serovars has been described previously.2526…”

The use of current genotyping assay methods for Neisseria gonorrhoeae

“…Достаточно распространенным методом геноти пирования является плазмидный анализ, который включает характеристику как полного плазмидно го профиля, так и специфических плазмид N. gonorrhoeae -cppB ген, плазмиды устойчивости к антибио тикам -tetM, семейство βлактамазопродуцирующих плазмид. плазмидный анализ используется в эпиде миологических исследованиях, при расследовании вспышек заболеваний [45][46][47][48][49][50][51] В 2012 г. R. Heymans и соавт. [53] выполнили ра боту по сравнению трех наиболее часто применяю щихся методов генотипирования N. gonorrhoeae -VNTR, NGMAST и porBсеквенирования.…”