Molecular and Epidemiologic Trends of Caliciviruses Associated with Outbreaks of Acute Gastroenteritis in the United States, 2000–2004

Blanton, Lenee; Adams, Susan M.; Beard, R. Suzanne; Wei, Guiwu; Bulens, Sandra N.; Widdowson, Marc‐Alain; Glass, Roger I.; Monroe, Stephan S.

doi:10.1086/499315

Cited by 242 publications

(196 citation statements)

References 26 publications

Supporting

Mentioning

182

Contrasting

Unclassified

Order By: Relevance

“…This is the predominant genotype associated with NoV gastroenteritis worldwide, including Brazil (13,26). Two strains (5037 and 5118) were grouped with reference strains belonging to GII/6, GII/7 and GII/9, whereas strain 5032 formed an independent cluster (Figure 2).…”

Section: Discussionmentioning

confidence: 99%

Detection of caliciviruses associated with acute infantile gastroenteritis in Salvador, an urban center in Northeast Brazil

Xavier

Oliveira

Ferreira

et al. 2009

Braz J Med Biol Res

View full text Add to dashboard Cite

Acute gastroenteritis caused by viruses is one of the leading causes of infantile morbidity. The aim of the present study was to investigate the presence of human caliciviruses of the genera norovirus and sapovirus in children up to 3 years of age with acute gastroenteritis from low-income communities in the city of Salvador, Brazil. This study is an extension of previous work carried out to establish the profile of the most prevalent enteric pathogens present in these communities. In this report, 139 fecal samples, collected from July 2001 to January 2002 were analyzed by RT-PCR and 13 (9%) were positive for human caliciviruses. By sequencing, seven isolates were characterized as norovirus genogroup GII and one as sapovirus genotype GII/1. Sequencing of the previously detected group-A rotaviruses and human astroviruses was also performed and revealed the circulation of rotavirus group A genotypes G1P[8] and G9P [8], and human astrovirus genotypes 6, 7, and 8. No mixed infection was observed. Community-based studies provide geographically representative information on disease burden. However, there are only a few reports in developing countries concerning the genotypes of the most important gastroenteric viruses detected in such communities. The present findings demonstrate the wide diversity of genotypes of the most important viruses responsible for acute gastroenteritis circulating in low-income communities.

show abstract

Section: Discussionmentioning

confidence: 99%

Detection of caliciviruses associated with acute infantile gastroenteritis in Salvador, an urban center in Northeast Brazil

Xavier

Oliveira

Ferreira

et al. 2009

Braz J Med Biol Res

View full text Add to dashboard Cite

show abstract

“…MNV is related to human noroviruses (HuNoV), which cause most of the sporadic cases and outbreaks of infectious nonbacterial gastroenteritis worldwide in people of all ages (4,15,28,36,38,64). However, noroviruses are an understudied group of viruses due to the previous lack of a tissue culture system and small-animal model.…”

mentioning

confidence: 99%

High-Resolution X-Ray Structure and Functional Analysis of the Murine Norovirus 1 Capsid Protein Protruding Domain

Taube

Rubin

Katpally

et al. 2010

J Virol

127

View full text Add to dashboard Cite

Murine noroviruses (MNV) are closely related to the human noroviruses (HuNoV), which cause the majority of nonbacterial gastroenteritis. Unlike HuNoV, MNV grow in culture and in a small-animal model that represents a tractable model to study norovirus biology. To begin a detailed investigation of molecular events that occur during norovirus binding to cells, the crystallographic structure of the murine norovirus 1 (MNV-1) capsid protein protruding (P) domain has been determined. Crystallization of the bacterially expressed protein yielded two different crystal forms (Protein Data Bank identifiers [PDB ID], 3LQ6 and 3LQE). Comparison of the structures indicated a large degree of structural mobility in loops on the surface of the P2 subdomain. Specifically, the A-B and E-F loops were found in open and closed conformations. These regions of high mobility include the known escape mutation site for the neutralizing antibody A6.2 and an attenuation mutation site, which arose after serial passaging in culture and led to a loss in lethality in STAT1 ؊/؊ mice, respectively. Modeling of a Fab fragment and crystal structures of the P dimer into the cryoelectron microscopy three-dimensional (3D) image reconstruction of the A6.2/ MNV-1 complex indicated that the closed conformation is most likely bound to the Fab fragment and that the antibody contact is localized to the A-B and E-F loops. Therefore, we hypothesize that these loop regions and the flexibility of the P domains play important roles during MNV-1 binding to the cell surface.Murine noroviruses (MNV) are members of the family Caliciviridae, which contains small icosahedral viruses with positive-sense, single-stranded RNA genomes (18). MNV is related to human noroviruses (HuNoV), which cause most of the sporadic cases and outbreaks of infectious nonbacterial gastroenteritis worldwide in people of all ages (4,15,28,36,38,64). However, noroviruses are an understudied group of viruses due to the previous lack of a tissue culture system and small-animal model. Since its discovery in 2003 (23), MNV has become an increasingly important model to study norovirus biology (66). The availability of a small-animal model, cell culture, and reverse-genetics system, combined with many shared characteristics of human and murine noroviruses, allows detailed studies of norovirus biology (7,23,63,65,66).The norovirus genome is organized into 3 major open reading frames (ORFs), which encode the nonstructural polyprotein (ϳ200 kDa) and the major (VP1; ϳ58-kDa) and minor (VP2; ϳ20-kDa) capsid proteins (18). Recently, a putative ORF-4 was identified in MNV, but the existence of that product and its function remain unknown (60). Norovirus capsids are formed from 180 copies of VP1 arranged with Tϭ3 icosahedral symmetry (9,25,(46)(47)(48). Each capsid protein is divided into an N-terminal arm (N), a shell (S), and a C-terminal protruding (P) domain, with the last two domains connected by a short hinge. VP1 self-assembles into virus-like particles (VLPs) in baculovirus, mammalian, and plant e...

show abstract

“…The outbreak strain was assigned, by sequence homology analysis, to the GII.4 variant 2010, considered the pandemic variant responsible of the epidemic events that occurred worldwide during 2009-2010 [Mathijs et al, 2011;Vega et al, 2011]. Over the last decade five NoV pandemic events have been reported associated with the genotype GII.4 and each of them have been trigged from a mutated strain [Blanton et al, 2006;Bull et al, 2006;Tu et al, 2008;Siebenga et al, 2009]. In the absence of a reliable cell culture system, the RT-PCR is considered the gold standard for NoV detection.…”

Section: Characterization Of the Outbreak In Ballshmentioning

confidence: 99%

Molecular characterization of norovirus GII strains identified in Albania

Donia

Cenko²,

Divizia

2013

Journal of Medical Virology

View full text Add to dashboard Cite

Noroviruses (NoVs) are considered as the leading cause of diarrheal diseases in all groups of age. In the last decade the number of NoV outbreaks worldwide is increasing. Data published by the systems of NoV surveillance show the GII.4 strain as the human predominant genotype circulating worldwide and new genetic variants of GII.4 were associated with epidemic events. In Albania the economy transformation has damaged significantly the environment and a large circulation of enteric viruses was reported in the past with the presence of NoV among the genotyped strains. This study aimed to characterize, by molecular analysis, the NoV GII strains detected in Albania during two time periods: in 2010 from the outbreak occurred in Ballsh and in 2002–2003 from sporadic cases of diarrhoea. A total of 21 Nov GII strains were characterized. The NoV GII.4 was genotyped more frequently and it was related closely to the pandemic variants recorded in GenBank. During 2002–2003, six NoV GII recombinant strains have been characterized

show abstract

Molecular and Epidemiologic Trends of Caliciviruses Associated with Outbreaks of Acute Gastroenteritis in the United States, 2000–2004

Cited by 242 publications

References 26 publications

Detection of caliciviruses associated with acute infantile gastroenteritis in Salvador, an urban center in Northeast Brazil

Detection of caliciviruses associated with acute infantile gastroenteritis in Salvador, an urban center in Northeast Brazil

High-Resolution X-Ray Structure and Functional Analysis of the Murine Norovirus 1 Capsid Protein Protruding Domain

Molecular characterization of norovirus GII strains identified in Albania

Contact Info

Product

Resources

About