Molecular and ecological study of Eryngium species in Syria

Jawdat, Dana; Al-Faoury, Hussam; Ayyoubi, Zuhair; Al-Safadi, B.

doi:10.2478/s11756-010-0086-7

Cited by 12 publications

(12 citation statements)

References 12 publications

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“…For Citrus species IRAP-markers somewhat yielded to RAPD-and ISSRmarkers by the level of detected variation: the proportion of polymorphic bands constituted 82.4%, 92.1% and 84.9%, respectively, while the expected heterozygosity -0.24, 0.29 and 0.22 (Biswas et al 2010). IRAPand RAPD-PCR showed approximately equal efficiency for differentiating the Eryngium species (Jawdat et al 2010), while IRAP-markers proved to be more effective than ISSR for polymorphism detection in tobacco (Nicotiana tabacum L.) and grapevine (Vitis vinifera L.) (Tao et al 2009;D'Onofrio et al 2010). RGAPand RAPD-markers revealed almost identical level of polymorphism in population analysis of Pinus oocarpa (Díaz & Ferrer 2003).…”

Section: Discussionmentioning

confidence: 96%

Efficiency of different PCR-based marker systems for assessment of Iris pumila genetic diversity

et al. 2013

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Abstract:We investigated informativeness and effectiveness of different marker types (ISSR, IRAP, REMAP, RGAP and LP-PCR that employ primers based on the conservative sequences of abiotic stress response genes) to study genetic diversity of Iris pumila L. By the number of amplicons per primer, number of polymorphic amplicons per primer and resolving power index (Rp), ISSR-markers were the most efficient followed by LP-PCR-markers. In order of decreasing value of indicators of genetic diversity "the percentage of polymorphic bands", and "the average Jaccard's genetic distance between plants", marker systems may be arranged as follows: ISSR > RAPD > LP-PC > RGAP ≈ IRAP. For ISSR-markers, the percentage of polymorphic bands was 1.3-1.7 times higher than for the others, and the average genetic distance was 1.2-1.3 times higher. Different marker systems were ranked by the value of Nei's gene diversity and the Shannon's index as follows: ISSR > RAPD ≈ LP-PCR > RGAP ≈ IRAP, with the highest and the lowest values differing 1.4 times. Genetic population structure was investigated with program Structure 2.3. The data of all marker systems suggest that all genomes under study belonged to one population. The PCoA and cluster analyses based on genetic distances showed distinctions in clustering generated from different markers data and summarized data, as well as the lack of strong clusters. Mantel test revealed significant positive correlation between the matrices of genetic distances generated by the data of almost all marker systems. The strongest correlation was found between RGAP-and IRAP-markers (r = 0.452, p = 0.01) and between RGAP and ISSR (r = 0.430, p = 0.01). ISSR, RAPD and LP-PCR proved to be more effective for the study of I. pumila genetic diversity, nevertheless, joint use of different marker systems will provide a more comprehensive assessment of variation in different genomic regions.

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Section: Discussionmentioning

confidence: 96%

Efficiency of different PCR-based marker systems for assessment of Iris pumila genetic diversity

et al. 2013

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“…On the southern Mediterranean coast, it is recorded from Algeria, Egypt, Libya, Morocco and Tunisia (Turmel 1948;Hanifi, Kadik & Guittonneau 2007). In western Asia, it occurs on Cyprus and Crete, and in Georgia, Israel, Lebanon, Syria and Turkey (Chater 1968;Lakkis et al 1996;Jawdat et al 2010;USDA, ARS National Genetic Resources Program 2010;Akalin Urus ßak et al 2013).…”

Section: Geographical and Altitudinal Distributionmentioning

confidence: 99%

Biological Flora of the British Isles: Eryngium maritimum

Isermann

Rooney

2014

Journal of Ecology

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Summary1. This account presents information on all aspects of the biology of Eryngium maritimum L. (Sea Holly) that are relevant to understanding its ecological characteristics and behaviour. The main topics are presented within the standard framework of the Biological Flora of the British Isles: distribution, habitat, communities, responses to biotic factors, responses to environment, structure and physiology, phenology, floral and seed characters, herbivores and disease, history and conservation. 2. Eryngium maritimum is a native perennial hemicryptophyte, with a large taproot, spiny and leathery leaves and a pale bluish inflorescence. It has a more or less continuous distribution in suitable habitats along the coasts of Great Britain and Ireland up to about 55°N, but it is more scattered further north. On the west coast, it is found south of the Hebrides, and on the east coast, with some exceptions, south of Yorkshire. In Europe, it has a wide, but mainly southern temperate, European distribution along the coasts of the Atlantic Ocean, the Baltic, the Mediterranean, and the Black and Azov Seas. Its northern distribution limit is at c. 60°N. 3. Eryngium maritimum grows typically on sand and shingle beaches, foredunes and yellow dunes, as well as in semi-fixed grey dunes. Its habitats have full sunlight and are more or less dry. It occurs in many coastal plant communities from the beach inland, and because of its wide European distribution, it occurs with members of several different biogeographical species groups. 4. Protected from grazing by its spininess and sclerophylly, E. maritimum is nevertheless vulnerable to direct damage by trampling. It supports few insect herbivores, probably because of chemical defences. Historically, it has had a great number of medicinal uses. 5. Eryngium maritimum is unable to withstand competition from faster and more densely growing plant species. In many coastal regions, in both temperate and mediterranean parts of Europe, it is one of the rarest and most threatened plant species, mainly because of habitat loss and land-use changes.Key-words: climatic limitation, communities, conservation, ecophysiology, geographical and altitudinal distribution, germination, herbivory, mycorrhiza, reproductive biology, soils Sea Holly. Apiaceae subfamily Saniculoideae. A long-lived, perennial herb with a long, well-developed taproot and thick and fleshy rhizomes extending laterally from buried stems. Reproductive stems 0.15-0.60 m; lower part often root-like and buried in the sand; aerial part pale below but with bluish tinge above, erect, robust, terete or sulcate, glabrous, solid and widely branched above so that the plant forms an open cushion. Basal leaves several; lamina 4-10 9 15-20 cm, leathery, glabrous, intensely glaucous, subrotund in outline and palmately 3(À5) lobed; base truncate or cordate; venation palmate and reticulate, the veins thick, prominent; margin thick, cartilaginous, with large spinose teeth; petioles about as *Nomenclature of vascular plants follows Stace (2010) ...

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“…DNA was extracted from young leaves by the CTAB method (Murray, Thompson, 1980). ISSR and IRAP markers from various literature sources were chosen for DNA genotyping (Arzate-Fernandez et al, 2005;Jawdat et al, 2010;Krishna Parvathaneni et al, 2011;Yuying et al, 2011;Senkova et al, 2013;Suprun et al, 2014). A total of 32 ISSR markers and 13 IRAP markers were used.…”

Section: Methodsmentioning

confidence: 99%

Analysis of genetic relationships of genotypes of the genus Rosa L. from the collection of Nikita Botanical Gardens using ISSR and IRAP DNA markers

et al. 2020

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In connection with the development of breeding and the creation of new plant varieties, the problem of their genotyping and identification is becoming increasingly important, therefore the use of molecular methods to identify genetic originality and assess plant genetic diversity appears to be relevant. As part of the work performed, informative ISSR and IRAP DNA markers promising for the study of genetic diversity of the Rosa L. genus were sought and applied to analysis of genetic relationships among 26 accessions of the genus Rosa L. from the gene pool collection of Nikita Botanical Gardens. They included 18 cultivated varieties and 8 accessions of wild species. The species sample included representatives of two subgenera, Rosa and Platyrhodon. The subgenus Platyrhodon was represented by one accession of the species R. roxburghii Tratt. Cultivated roses were represented by varieties of garden groups hybrid tea, floribunda, and grandiflora. The tested markers included 32 ISSRs and 13 IRAPs. Five ISSR markers (UBC 824, ASSR29, 3A21, UBC 864, and UBC 843) and three IRAPs (TDK 2R, Сass1, and Сass2) were chosen as the most promising. They were used for genotyping the studied sample of genotypes. In general, they appeared to be suitable for further use in studying the genetic diversity of the genus Rosa L. The numbers of polymorphic fragments ranged from 12 to 31, averaging 19.25 fragments per marker. For markers UBC 864 and UBC 843, unique fingerprints were identified in each accession studied. The genetic relationships of the studied species and varieties of roses analyzed by the UPGMA, PCoA, and Bayesian methods performed on the basis of IRAP and ISSR genotyping are consistent with their taxonomic positions. The genotype of the species R. roxburghii of the subgenus Platyrhodon was determined genetically as the most distant. According to clustering methods, the representative of the species R. bengalensis did not stand out from the group of cultivated varieties. When assessing the level of genetic similarity among the cultivated varieties of garden roses, the most genetically isolated varieties were ‘Flamingo’, ‘Queen Elizabeth’, and ‘Kordes Sondermeldung’; for most of the other varieties, groups of the greatest genetic similarity were identified. This assessment reflects general trends in phylogenetic relationships, both among the studied species of the genus and among cultivated varieties.

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Molecular and ecological study of Eryngium species in Syria

Cited by 12 publications

References 12 publications

Efficiency of different PCR-based marker systems for assessment of Iris pumila genetic diversity

Efficiency of different PCR-based marker systems for assessment of Iris pumila genetic diversity

Biological Flora of the British Isles: Eryngium maritimum

Analysis of genetic relationships of genotypes of the genus Rosa L. from the collection of Nikita Botanical Gardens using ISSR and IRAP DNA markers

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