Molecular and Cytological Comparisons of Chromosomes 7el&lt;sub&gt;1&lt;/sub&gt;, 7el&lt;sub&gt;2&lt;/sub&gt;, 7E&lt;sup&gt;e&lt;/sup&gt;, and 7E&lt;sup&gt;i&lt;/sup&gt; Derived from &lt;b&gt;&lt;i&gt;Thinopyrum&lt;/i&gt;&lt;/b&gt;

Guo, Jun; He, Fang; Cai, Jinjin; Wang, Hongwei; Li, An-Fei; Wang, Honggang; Kong, Lingrang

doi:10.1159/000381838

Cited by 11 publications

(8 citation statements)

References 41 publications

(52 reference statements)

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“…Previously, we mapped the Fhb7 to the distal end of the 7EL (based on recombination between 7E1 and 7E2 in a common wheat background) using a recombinant inbred line (RIL) population from a cross between an FHBsusceptible substitution line (7E1/7D) and an FHB-resistant substitution line (7E2/7D) (13,21). For further mapping of this gene, we developed a segregation population derived from BC 6 F 1 with the same cross, in which FHB resistance was tracked as monogenic trait for validation of phenotypes.…”

Section: Map-based Cloning Of the Fusarium Resistance Gene Fhb7mentioning

confidence: 99%

Horizontal gene transfer of Fhb7 from fungus underlies Fusarium head blight resistance in wheat

Wang

Sun

et al. 2020

Science

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Fusarium head blight (FHB), a fungal disease caused by Fusarium species that produce food toxins, currently devastates wheat production worldwide, yet few resistance resources have been discovered in wheat germplasm. Here, we cloned the FHB resistance gene Fhb7 by assembling the genome of Thinopyrum elongatum, a species used in wheat distant hybridization breeding. Fhb7 encodes a glutathione S-transferase (GST) and confers broad resistance to Fusarium species by detoxifying trichothecenes through de-epoxidation. Fhb7 GST homologs are absent in plants, and our evidence supports that Th. elongatum has gained Fhb7 through horizontal gene transfer (HGT) from an endophytic Epichloë species. Fhb7 introgressions in wheat confers resistance to both FHB and crown rot in diverse wheat backgrounds without yield penalty, providing a solution for Fusarium resistance breeding.

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Section: Map-based Cloning Of the Fusarium Resistance Gene Fhb7mentioning

confidence: 99%

Horizontal gene transfer of Fhb7 from fungus underlies Fusarium head blight resistance in wheat

Wang

Sun

et al. 2020

Science

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460

358

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“…ponticum . This strong FHB resistance QTL was pyramided into bread wheat (Shen and Ohm, 2007; Zhang et al, 2011; Forte et al, 2014) and also durum wheat (Forte et al, 2014) by exploiting the close relatedness of 7el 2 with 7el 1 (Dvorak, 1975; Guo et al, 2015a), deriving from a different accession of the same species. The latter bear genes for effective rust resistances ( Lr19 and Sr25 ) and for yield-contributing traits (see Ceoloni et al, 2015 for a review).…”

Section: Introductionmentioning

confidence: 99%

Equipping Durum Wheat—Thinopyrum ponticum Recombinant Lines With a Thinopyrum elongatum Major QTL for Resistance to Fusarium Diseases Through a Cytogenetic Strategy

et al. 2019

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Prompted by recent changes in climate trends, cropping areas, and management practices, Fusarium head blight (FHB), a threatening disease of cereals worldwide, is also spreading in unusual environments, where bread wheat (BW) and durum wheat (DW) are largely cultivated. The scarcity of efficient resistance sources within adapted germplasm is particularly alarming for DW, mainly utilized for human consumption, which is therefore at high risk of kernel contamination by health-dangerous mycotoxins (e.g., deoxynivalenol = DON). To cope with this scenario, we looked outside the wheat primary gene pool and recently transferred an exceptionally effective FHB resistance QTL (Fhb-7EL) from Thinopyrum elongatum 7EL chromosome arm onto a Thinopyrum ponticum 7el1L arm segment, containing additional valuable genes (including Lr19 for leaf rust resistance and Yp for yellow pigment content), distally inserted onto 7DL of BW lines. Two such lines were crossed with two previously developed DW-Th. ponticum recombinants, having 7el1L distal portions on 7AL arms. Genomic in situ hybridization (GISH) analysis showed homologous pairing, which is enabled by 7el1L segments common to the BW and DW recombinant chromosomes, to occur with 42-78% frequency, depending on the shared 7el1L amount. Aided by 7EL/7el1L-linked markers, 7EL+7el1L tetraploid recombinant types were isolated in BC1 progenies to DW of all cross combinations. Homozygous 7EL+7el1L recombinant plants and null segregates selected in BC2F2 progenies were challenged by Fusarium graminearum spike inoculation to verify the Fhb-7EL efficacy in DW. Infection outcomes confirmed previous observations in BW, with >90% reduction of disease severity associated with Fhb-7EL presence vs. its absence. The same differential effect was detected on seed set and weight of inoculated spikes, with genotypes lacking Fhb-7EL having ∼80% reduction compared with unaffected values of Fhb-7EL carriers. In parallel, DON content in flour extracts of resistant recombinants averaged 0.67 ppm, a value >800 times lower than that of susceptible controls. Furthermore, as observed in BW, the same Fhb-7EL also provided the novel DW recombinants with resistance to Fusarium crown rot (∼60% symptom reduction) as from seedling infection with Fusarium culmorum. Through alien segment stacking, we succeeded in equipping DW with a very effective barrier against different Fusarium diseases and other positive attributes for crop security and safety.

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“…ponticum chromosomes 7E1 and 7E2 in hybrids, with frequencies of meiotic pairing rates of 19.85 and 2.52, respectively ( 20 ). The genetic relationships based on molecular markers also revealed that 7E was distant from 7E1 and 7E2 ( 20 ). It was reported that resynthesized polyploids and natural polyploids have undergone many genetic changes, including sequence deletion, rDNA loci changes, transposon activation and chromosomal rearrangement ( 21–27 ).…”

Section: Discussionmentioning

confidence: 99%

“…elongatum chromosome 7E paired occasionally with Th. ponticum chromosomes 7E1 and 7E2 in hybrids, with frequencies of meiotic pairing rates of 19.85 and 2.52, respectively ( 23 ). The genetic relationships based on molecular markers also revealed that 7E was distant from 7E1 and 7E2 ( 23 ).…”

Section: Discussionmentioning

confidence: 99%

“…All these findings suggest that 7E and 7E2 have difference on the DNA level. Fhb7 was mapped to the distal end of 7E2 between the XSdauK79 and XSdauK80 markers based on recombinations between 7E1 and 7E2 ( 11, 23 ). Although ~1.2 Mb region between the two markers was identified on the 7E chromosome, the DNA components between the two mapping intervals of 7E and 7E2 might be quite different.…”

Section: Discussionmentioning

confidence: 99%

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Alien chromatin other than the GST-encodingFhb7candidate confers Fusarium head blight resistance in wheat breeding

Shi

Yuan

et al. 2021

Preprint

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Fusarium head blight (FHB), caused by Fusarium species, seriously threaten global wheat production. Three wheat-Th.elongatum FHB resistant translocation lines have been developed and used for breeding. Transcriptomic analysis identified a derivative glutathione S-transferase transcript T26102, which was homologous to Fhb7 and induced dramatically by Fusarium graminearum. Homologs of Fhb7 were detected in several genera in Triticeae, including Thinopyrum, Elymus, Leymus, Pseudoroegeria and Roegeria. Several wheat-Thinopyrum translocation lines carrying Fhb7 remain susceptible to FHB, and transgenic plants overexpressing the T26102 on different backgrounds did not improve the FHB resistance. Taken as a whole, we show the application of the chromatin derived from diploid Thinopyrum elongatum successfully conferring wheat with high level FHB resistance independent of the Fhb7.

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Molecular and Cytological Comparisons of Chromosomes 7el<sub>1</sub>, 7el<sub>2</sub>, 7E<sup>e</sup>, and 7E<sup>i</sup> Derived from <b><i>Thinopyrum</i></b>

Cited by 11 publications

References 41 publications

Horizontal gene transfer of Fhb7 from fungus underlies Fusarium head blight resistance in wheat

Horizontal gene transfer of Fhb7 from fungus underlies Fusarium head blight resistance in wheat

Equipping Durum Wheat—Thinopyrum ponticum Recombinant Lines With a Thinopyrum elongatum Major QTL for Resistance to Fusarium Diseases Through a Cytogenetic Strategy

Alien chromatin other than the GST-encodingFhb7candidate confers Fusarium head blight resistance in wheat breeding

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