Molecular and cytological characterization of the global Musa germplasm collection provides insights into the treasure of banana diversity

Christelová, Pavla; Langhe, Edmond De; Hřibová, Eva; Čížková, Jana; Sardos, Julie; Hušáková, Markéta; houwe, Ines Van den; Sutanto, Agus; Kepler, A.K.; Swennen, Rony; Roux, Nicolás; Doležel, Jaroslav

doi:10.1007/s10531-016-1273-9

Cited by 106 publications

(167 citation statements)

References 66 publications

(89 reference statements)

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“…The figure shows the position of screened accessions (with a red dot and highlighted in yellow) on the dendogram and how they clustered with the core subsets identified by Christelová et al . (). The different blocks of accessions are differentiated by colour.…”

Section: Resultsmentioning

confidence: 99%

“…The homogenate was filtered through a 50 μm nylon filter into a sample tube and incubated for 1–5 min on ice. Otto II buffer (1 mL) containing the fluorescent dye 4‐6‐diamidino phenyl indole (DAPI) was added before ploidy measurement using Sysmex CyFlow flow cytometer equipped with UV excitation and detectors for DAPI fluorescence (Christelová et al ., ). Molecular characterization using SSR markers targeting 19 loci was conducted as described by Christelová et al .…”

Section: Methodsmentioning

confidence: 99%

“…Molecular characterization using SSR markers targeting 19 loci was conducted as described by Christelová et al . (, ). The SSR markers are motifs of 1–6 bp repeats tandemly arranged in the genomes of eukaryotic and prokaryotic organisms (Christelová et al ., ).…”

Section: Methodsmentioning

confidence: 99%

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Sources of resistance in Musa to Xanthomonas campestris pv. musacearum, the causal agent of banana xanthomonas wilt

et al. 2018

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It is claimed that, with the exception of Musa balbisiana, all banana varieties are susceptible to bacterial wilt caused by Xanthomonas campestris pv. musacearum (Xcm). Despite being resistant to Xcm infection, M. balbisiana is not preferred for breeding because it belongs to the BB genome subgroup, while most edible bananas are of the A genome. To identify potential sources of resistance to Xcm, 72 banana accessions representing the Musa genetic diversity were evaluated in an outdoor confined potted trial. The midribs of the youngest leaf of 3‐month‐old banana plants were inoculated with 108 CFU mL−1 of Xcm isolate USY13P, and symptom development assessed weekly for 4 months. Results confirmed that M. balbisiana genotypes are indeed resistant to Xcm. Varieties within the Musa acuminata subsp. zebrina (AA) set were further identified as potentially useful sources of Xcm resistance. These findings reveal the potential to develop banana and plantain varieties with tolerance to Xcm.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Sources of resistance in Musa to Xanthomonas campestris pv. musacearum, the causal agent of banana xanthomonas wilt

et al. 2018

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“…These results are similar to report by Samarasinghat et al (2010), where in the morphological and SSR clustering, AAB genotypes well as all other genotypes occurred in different groups. A study by Christelová et al 2016 used Molecular and Cytological systems to characterise global banana collections recommended more focus on molecular and morphological evaluation.…”

Section: Discussionmentioning

confidence: 99%

Unravelling genetic make-up of some Musa hybrids and selected Musa accessions using molecular and morphological characterization

Quain¹,

Agyeman²,

Okyere³

et al. 2018

Int. J. Genet. Mol. Biol.

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Plantains are important staples in sub-Saharan Africa, however, production has been saddled by several constraints including pests and diseases and lack of improved technologies. In regard to genetic study, morphological characterization has identified about 100 local accessions to exist in Ghana. However, limited molecular characterization has been done on plantains. Characterization based on morphologic characteristics alone may be limited since expression of quantitative traits is subjective to strong environmental influence. Alternatively, molecular characterization techniques are capable of identifying polymorphism represented by differences in DNA sequences. The objective of this research was therefore to characterize local accessions of plantain using morphological and molecular techniques. This study sampled 38 local accessions from two regions in Ghana for characterization, taking into account all known morphological characteristics. Genomic DNA was isolated for molecular characterization using 18 SSR markers and 10 random primers. The SSR markers revealed 52 alleles, random primers revealed 67 alleles and the 29 morphological traits (loci) had 79 attributes (alleles). Similarity matrices of SSR markers revealed that 26.5% of the plantain accessions were duplicates; however, random amplified polymorphic DNA (RAPDs) system and morphological marker systems did not reveal any duplication among genotypes. Dendrogram generated on bases of both SSR and RAPDs indicated that a French plantain accession (Apem 01) and a False Horn accession (Apantu 26) were the most distantly related collections. None of the molecular marker methods used clearly clustered various phenotypically known collections into their respective distinct groups, although to some extent, this was demonstrated by morphological data set.

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“…To date, the genomes of two banana progenitors, M. acuminata subsp. malaccensis (A genome) and M. balbisiana (B genome), and another wild banana, M. itinerans , have been sequenced with the resulting sequences revealing genome evolution peculiarities of Musaceae (D'Hont et al ., ; Davey et al ., ; Čížková et al ., ; Christelová et al ., ). This information could shed more light on Musa spp.…”

Section: Understanding the Host Response To Pseudocercospora Banana Pmentioning

confidence: 99%

Progress in understanding Pseudocercospora banana pathogens and the development of resistant Musa germplasm

et al. 2018

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Banana and plantain (Musa spp.) are important food crops in tropical and subtropical regions of the world where they generate millions of dollars annually to both subsistence farmers and exporters. Since 1902, Pseudocercospora banana pathogens, Pseudocercospora fijiensis, P. musae and P. eumusae, have emerged as major production constraints to banana and plantain. Despite concerted efforts to counter these pathogens, they have continued to negatively impact banana yield. In this review, the economic importance, distribution and the interactions between Pseudocercospora banana pathogens and Musa species are discussed. Interactions are further scrutinized in the light of an emerging climate change scenario and efforts towards the development of resistant banana germplasm are discussed. Finally, some of the opportunities and gaps in knowledge that could be exploited to further understanding of this ubiquitous pathosystem are highlighted.

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Molecular and cytological characterization of the global Musa germplasm collection provides insights into the treasure of banana diversity

Cited by 106 publications

References 66 publications

Sources of resistance in Musa to Xanthomonas campestris pv. musacearum, the causal agent of banana xanthomonas wilt

Sources of resistance in Musa to Xanthomonas campestris pv. musacearum, the causal agent of banana xanthomonas wilt

Unravelling genetic make-up of some Musa hybrids and selected Musa accessions using molecular and morphological characterization

Progress in understanding Pseudocercospora banana pathogens and the development of resistant Musa germplasm

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