Molecular- and Culture-based Comparison of the Effects of Antimicrobial Agents on Bacterial Survival in Infected Dentinal Tubules

Cook, Jerry L.; Nandakumar, Renu; Fouad, Ashraf F.

doi:10.1016/j.joen.2007.01.022

Cited by 38 publications

(46 citation statements)

References 39 publications

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“…Accordingly, the first and second tubes containing serial dilutions received a neutralizing solution to prevent transfer of irrigant residues into the culture medium, thus assuring that the irrigation solution would not interfere with bacterial growth for longer than the predetermined experimental period (20). Previous studies have demonstrated the efficacy of 2% CHX against E. faecalis (11,12), but the results in the literature are conflicting, probably because of methodological differences (11,18). We observed similar antibacterial effect for 2% CHX and 2.5% NaOCl.…”

Section: Discussionmentioning

confidence: 99%

“…The use of CHX as an endodontic irrigant has also been indicated due to its broad spectrum of antimicrobial activity and substantivity (10). The efficacy of 2% CHX against E. faecalis has been demonstrated both in vitro and in vivo (11,12). However, its main disadvantage, the lack of organic matter-dissolving ability (13), may compromise its cleaning effectiveness (14).…”

Section: Introductionmentioning

confidence: 99%

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Antibacterial effectiveness of peracetic acid and conventional endodontic irrigants

et al. 2011

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This study evaluated the in vitro antibacterial activity of conventional and experimental endodontic irrigants against Enterococcus faecalis. The following substances were evaluated by direct contact test: 2.5% sodium hypochlorite (NaOCl); 2% chlorhexidine (CHX); 1% peracetic acid. After different contact periods (30 s, 1, 3, and 10 min), a neutralizing agent was applied. Serial 10-fold dilutions were prepared and plated onto tryptic soy agar (TSA) and the number of colony-forming units per milliliter (CFU/mL) was determined. Sterile saline was used as a negative control. Both 2.5% NaOCl and 2% CHX eliminated E. faecalis after 30 s of contact. Peracetic acid reduced the bacterial counts by 86% after 3 min and completely eliminated E. faecalis after 10 min. These results allow us to conclude that 1% peracetic acid is effective against E. faecalis, despite its slower action compared with 2.5% NaOCl and 2% CHX.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antibacterial effectiveness of peracetic acid and conventional endodontic irrigants

et al. 2011

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“…Chlorhexidine is also very effective for root canal disinfection and it has a better effect on Candida albicans than sodium hypochlorite (8). Effect of 2% chlorhexidine on E. faecalis has been demonstrated in in vitro and in vivo conditions (9,10). The main dissadvantage is its poor organic structure removal (10), which reduces its ability to clean and remove debris from the root canal (11).…”

Section: Janković I Surmentioning

confidence: 99%

“…Istaknimo da se klorheksidin pokazao kao vrlo učinkovito sredstvo za dezinfekciju korijenskih kanala, a također bolje djeluje na bakteriju Candida albicans nego natrijev hipoklorit (8). Učinak 2-postotnog klorheksidina na Enterococus faecalis dokazan je u uvjetima in vitro i in vivo (9,10). Glavni mu je nedostatak to što loše uklanja organske tvari (11), što smanjuje njegovu sposobnost čišćenja i uklanjanja debrisa iz korijenskog kanala (12).…”

Section: Janković I Surunclassified

Antimicrobial Effectiveness of Intracanal Ozone Treatment

Janković

Klarić

Prskalo

et al. 2013

Acta Stomatol Croat

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“…Cook et al evaluated the quality of root canal filling with or without calcium hydroxide application prior to the root canal filling or 2% chlorhexidine on the persistence of bacterial infection on the dentinal tubules, they found that the use of 2% chlorhexidine followed by root canal filling was more effective in removing the bacterial infection especially E. faecalis than placement of calcium hydroxide or immediate canal filling [12]. Peters et al claimed that the number of positive gram bacteria increased in root canal system after dressing with calcium hydroxide [13].…”

Section: Antibacterial Effectmentioning

confidence: 99%

Calcium Hydroxide in Endodontics: An Overview

Ba-Hattab¹,

Al-Jamie²,

Aldreib³

et al. 2016

OJST

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Background: Calcium hydroxide has been used in dentistry since several decades. It has been used in a number of applications in the field of endodontics such as root resorption, intracanal medicament, and root canal sealers. Although this material exhibits several advantages, it also has some limitations. Objectives: To review the role of calcium hydroxide in the field of endodontics, focusing on its mechanism of action, antimicrobial effects, different applications, cytotoxicity or biocompatibility, and its removal from the root canals. Materials and Methods: An electronic search was done using different databases. Out of 2,664 articles, only 33 articles have been selected to be included in this review because they are directly related to the topic and matched the inclusion criteria of this review: "Language: English" and "Year: 2000-2016". Results: The antimicrobial effect of calcium hydroxide is controversial. Although some studies supported the effectiveness of calcium hydroxide against some bacteria others reported its limitation against fungal infection. Calcium hydroxide can be used effectively as intracanal medicament, root canal sealer, in weeping canals, for perforation management and root resorption. Conclusions: Despite the limitation of antimicrobial activity of calcium hydroxide, it is used effectively in a number of treatment modalities in endodontics. Due to its biological and therapeutical properties, calcium hydroxide is the material of choice for all pulp therapy. However, when using calcium hydroxide as a dressing material in root canal treatment caution should be taken to prevent the overextension of the paste beyond the tooth apex and avoid the harmful side effects.

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Molecular- and Culture-based Comparison of the Effects of Antimicrobial Agents on Bacterial Survival in Infected Dentinal Tubules

Cited by 38 publications

References 39 publications

Antibacterial effectiveness of peracetic acid and conventional endodontic irrigants

Antibacterial effectiveness of peracetic acid and conventional endodontic irrigants

Antimicrobial Effectiveness of Intracanal Ozone Treatment

Calcium Hydroxide in Endodontics: An Overview

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