Molecular and Crystal Structure of Spiculisporic Acid and Correlation with the Surface Activity

Ishigami, Yutaka; Zhang, Yuejun; Goto, Midori

doi:10.5650/jos.62.795

Cited by 2 publications

(1 citation statement)

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“…Spiculisporic acid (4,5-dicarboxy-4-pentadecanolide, SA) is a fatty acid-type biosurfactant (Ishigami et al 2013 ). The tricarboxylic-type surfactant structure has recently received attention as a commercially available biosurfactant and a new biomaterial owing to its safety for human health and the environment.…”

Section: Introductionmentioning

confidence: 99%

Production of spiculisporic acid by Talaromyces trachyspermus in fed-batch bioreactor culture

Moriwaki-Takano

Asada

Nakamura

2021

Bioresour. Bioprocess.

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Spiculisporic acid (SA) is a fatty acid-type biosurfactant with one lactone ring and two carboxyl groups. It has been used in metal removers and cosmetics, because of its low propensity to cause irritation to the skin, its anti-bacterial properties, and high surface activity. In the present study, we report an effective method for producing SA by selecting a high-producing strain and investigating the effective medium components, conditions, and environments for its culture. Among the 11 kinds of Talaromyces species, T. trachyspermus NBRC 32238 showed the highest production of a crystalline substance, which was determined to be SA using NMR. The strain was able to produce SA under acidic conditions from hexoses, pentoses, and disaccharides, with glucose and sucrose serving as the most appropriate substrates. Investigation of nitrogen sources and trace metal ions revealed meat extract and FeCl3 as components that promoted SA production. Upon comparing the two types of cultures with glucose in a baffle flask or aeration bioreactor, SA production was found to be slightly higher in the flask than in the reactor. In the bioreactor culture, sucrose was found to be an appropriate substrate for SA production, as compared to glucose, because with sucrose, the lag time until the start of SA production was shortened. Finally, fed-batch culture with sucrose resulted in 60 g/L of SA, with a total yield of 0.22 g SA/g sucrose and a productivity of 6.6 g/L/day.

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Section: Introductionmentioning

confidence: 99%