Molecular and clinical characterization of hypervirulent Klebsiella pneumoniae isolates from individuals with urinary tract infections

Li, Jun; Tang, Mingxi; Liu, Zhaojun; Xia, Fengjun; Min, Changhang; Hu, Yongmei; Wang, Haichen; Zou, Ming-Xiang

doi:10.3389/fcimb.2022.925440

Cited by 4 publications

(2 citation statements)

References 40 publications

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“…Here, we demonstrate a novel role for TerC as a fitness factor during a urinary tract infection. The identification of TerC as a bladder fitness factor using a hypervirulent Kp strain (NTUH-K2044) is noteworthy, as several recent reports have indicated that hvKp are an important cause of asymptomatic bacteriuria and UTIs ( 56 – 60 ). As hypervirulent strains become more prevalent and hypervirulent, antibiotic resistant strains emerge, and it is critical to identify compartment-specific fitness factors ( 61 , 62 ).…”

Section: Discussionmentioning

confidence: 99%

The Klebsiella pneumoniae ter Operon Enhances Stress Tolerance

et al. 2023

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Healthcare-acquired infections are a leading cause of disease in patients that are hospitalized or in long-term-care facilities. Klebsiella pneumoniae (Kp) is a leading cause of bacteremia, pneumonia, and urinary tract infections in these settings. Previous studies have established that the ter operon, a genetic locus that confers tellurite oxide (K 2 TeO 3 ) resistance, is associated with infection in colonized patients.

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Section: Discussionmentioning

confidence: 99%

The Klebsiella pneumoniae ter Operon Enhances Stress Tolerance

et al. 2023

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“…The identification of TerC as a bladder fitness factor using a hypervirulent Kp strain (NTUH-K2044) is noteworthy, as several recent reports have indicated that hvKp are an important cause of asymptomatic bacteriuria and UTI (56)(57)(58)(59)(60). As hypervirulent strains become more prevalent and hypervirulent, antibiotic resistant strains emerge, it is critical to identify compartment-specific fitness factors (61,62).…”

Section: Discussionmentioning

confidence: 99%

TheKlebsiella pneumoniae teroperon enhances stress tolerance

Mason

Vornhagen

Smith

et al. 2022

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Healthcare-acquired infections are a leading cause of disease in patients that are hospitalized or in long-term care facilities. Klebsiella pneumoniae (Kp) is a leading cause of bacteremia, pneumonia, and urinary tract infections in these settings. Previous studies have established that the ter operon, a genetic locus that confers tellurite oxide (K2TeO3) resistance, is associated with infection in colonized patients. Rather than enhancing fitness during infection, the ter operon increases Kp fitness during gut colonization; however, the biologically relevant function of this operon is unknown. First, using a murine model of urinary tract infection, we demonstrate a novel role for the ter operon protein TerC as a bladder fitness factor. To further characterize TerC, we explored a variety of functions, including resistance to metal-induced stress, resistance to ROS-induced stress, and growth on specific sugars, all of which were independent of TerC. Then, using well-defined experimental guidelines, we determined that TerC is necessary for tolerance to ofloxacin, polymyxin B, and cetylpyridinium chloride. We used an ordered transposon library constructed in a Kp strain lacking the ter operon to identify genes required to resist K2TeO3- and polymyxin B-induced stress, which suggested that K2TeO3-induced stress is experienced at the bacterial cell envelope. Finally, we confirmed that K2TeO3 disrupts the Kp cell envelope, though these effects are independent of ter. Collectively, the results from these studies indicate a novel role for the ter operon as stress tolerance factor, therefore explaining its role in enhancing fitness in the gut and bladder.

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