Molecular and cellular effects of contamination in aquatic ecosystems

Hampel, Miriam; Blasco, Julián; Segner, Helmut

doi:10.1007/s11356-015-5565-5

Cited by 28 publications

(9 citation statements)

References 35 publications

(28 reference statements)

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“…The prolonged and persistent exposure of living organisms to environmental contaminants may cause anomalies at the molecular level [70], where petroleum hydrocarbons are identified as carcinogenic and mutagenic pollutants [71]. Although DNA molecular markers are mostly used for the detection of variations in the studies of phylogeny, genetic diversity, genome mapping, gene tagging, evolution, and molecular ecology [72,73], DNA damage can be monitored accurately by using molecular markers [72].…”

Section: Discussionmentioning

confidence: 99%

Eco-physiological response and genotoxicity induced by crude petroleum oil in the potential phytoremediator Vinca rosea L

Hussein

Hegazy

Saber

et al. 2022

Journal of Genetic Engineering and Biotechnology

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Background Phytoremediation is determined as an emerging green technology suitable for the safe remediation and restoration of polluted terrestrial and aquatic environments. In this study, the assessment of an ornamental plant, Vinca rosea L., as a phytoremediator of crude oil in polluted soils was conducted. In an open greenhouse experiment, plants were raised in sandy-clayey soils treated with 1, 3, 5, and 7% oil by weight. The experiment was conducted over 5 months. Results Total petroleum hydrocarbon (TPH) degradation percentage by V. rosea after a 5-month growth period ranged from 86.83 ± 0.44% to 59.05% ± 0.45% in soil treated with 1 and 7%, respectively. Plants raised in polluted soils demonstrated a dramatic reduction in germination rates, in addition to growth inhibition outcomes shown from decreased plant height. An increase in branching was observed with an increase in oil pollution percentages. Moreover, the phytomass allocated to the leaves was higher, while the phytomass witnessed lower values for fine roots, flowering and fruiting when compared to the controls. Apart from the apparent morphological changes, there was a decrease in chlorophyll a/b ratio, which was inversely proportional to the oil pollution level. The contents of carotenoids, tannins, phenolics, flavonoids, and antioxidant capacity were elevated directly with an increase in oil pollution level. The start codon-targeted (SCoT) polymorphisms and inter-simple sequence repeat (ISSR) primers showed the molecular variations between the control and plants raised in polluted soils. The genetic similarity and genomic DNA stability were negatively affected by increased levels of crude oil pollution. Conclusions The ability of V. rosea to degrade TPH and balance the increased or decreased plant functional traits at the macro and micro levels of plant structure in response to crude oil pollution supports the use of the species for phytoremediation of crude oil-polluted sites. The genotoxic effects of crude oil on V. rosea still require further investigation. Further studies are required to demonstrate the mechanism of phenolic, flavonoid, and antioxidant compounds in the protection of plants against crude oil pollution stress. Testing different molecular markers and studying the differentially expressed genes will help understand the behavior of genetic polymorphism and stress-resistant genes in response to crude oil pollution.

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Section: Discussionmentioning

confidence: 99%

Eco-physiological response and genotoxicity induced by crude petroleum oil in the potential phytoremediator Vinca rosea L

Hussein

Hegazy

Saber

et al. 2022

Journal of Genetic Engineering and Biotechnology

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“…This is related to a paradigm change in ecotoxicology that is driven by mainly two issues: One issue is that ecotoxicology is confronted with a steadily growing number of chemicals and environmental samples to be tested. This can no longer be mastered by conventional in vivo testing approaches but necessitates the use of rapid and cost-effective technologies to screen, rank and prioritize the huge number of test agents and to alert for potential hazards [ 4 , 49 , 50 , 52 , 72 ]. Here, in vitro systems are the method of choice for the rapid profiling of the hazardous properties of chemicals and environmental samples.…”

Section: In Vitro Application Domains In Ecotoxicology: What Kind Of mentioning

confidence: 99%

In vitro or not in vitro: a short journey through a long history

2018

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The aim of ecotoxicology is to study toxic effects on constituents of ecosystems, with the protection goal being populations and communities rather than individual organisms. In this ecosystem perspective, the use of in vitro methodologies measuring cellular and subcellular endpoints at a first glance appears to be odd. Nevertheless, more recently in vitro approaches gained momentum in ecotoxicology. In this article, we will discuss important application domains of in vitro methods in ecotoxicology. One area is the use of in vitro assays to replace, reduce, and refine (3R) in vivo tests. Research in this field has focused mainly on the use of in vitro cytotoxicity assays with fish cells as non-animal alternative to the in vivo lethality test with fish and on in vitro biotransformation assays as part of an alternative testing strategy for bioaccumulation testing with fish. Lessons learned from this research include the importance of a critical evaluation of the sensitivity, specificity and exposure conditions of in vitro assays, as well as the availability of appropriate in vitro-in vivo extrapolation models. In addition to this classical 3R application, other application domains of in vitro assays in ecotoxicology include the screening and prioritization of chemical hazards, the categorization of chemicals according to their modes of action and the provision of mechanistic information for the pathway-based prediction of adverse outcomes. The applications discussed in this essay may highlight the potential of in vitro technologies to enhance the environmental hazard assessment of single chemicals and complex mixtures at a reduced need of animal testing.

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“…There are over 100 million substances registered in the Chemical Abstract Service, a significant portion of which are widely used in agriculture, industry and by individuals [ 4 ], and these substances are further concentrated in rivers and lakes. Due to possible toxicity to different inhabitants of water reservoirs, these chemicals should generally be tested for their biological effects at the cellular to ecosystem levels [ 5 ] to prevent or minimize negative impacts on the environment [ 6 ]. A number of environmental disasters, such as massive fish deaths in Louisiana in 1950s caused by uncontrolled use of the insecticide endrin in agriculture, exemplify the importance of preliminary ecotoxicological testing [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Microencapsulated fluorescent pH probe as implantable sensor for monitoring the physiological state of fish embryos

et al. 2017

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In vivo physiological measurement is a major challenge in modern science and technology, as is environment conservation at the global scale. Proper toxicological testing of widely produced mixtures of chemicals is a necessary step in the development of new products, allowing us to minimize the human impact on aquatic ecosystems. However, currently available bioassay-based techniques utilizing small aquatic organisms such as fish embryos for toxicity testing do not allow assessing in time the changes in physiological parameters in the same individual. In this study, we introduce microencapsulated fluorescent probes as a promising tool for in vivo monitoring of internal pH variation in zebrafish embryos. The pH alteration identified under stress conditions demonstrates the applicability of the microencapsulated fluorescent probes for the repeated analysis of the embryo’s physiological state. The proposed approach has strong potential to simultaneously measure a range of physiological characteristics using a set of specific fluorescent probes and to finally bring toxicological bioassays and related research fields to a new level of effectiveness and sensitivity.

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Molecular and cellular effects of contamination in aquatic ecosystems

Cited by 28 publications

References 35 publications

Eco-physiological response and genotoxicity induced by crude petroleum oil in the potential phytoremediator Vinca rosea L

Eco-physiological response and genotoxicity induced by crude petroleum oil in the potential phytoremediator Vinca rosea L

In vitro or not in vitro: a short journey through a long history

Microencapsulated fluorescent pH probe as implantable sensor for monitoring the physiological state of fish embryos

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