Molecular and Cellular Aspects of Mammalian Sperm Acrosomal Exocytosis

Spina, Florenza Antonella la; Stival, Cintia; Krapf, Darío; Buffone, Mariano G.

doi:10.1002/9781118881286.ch15

Cited by 4 publications

(4 citation statements)

References 216 publications

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“…Other studies used Septin4-null sperm or flagella modified with no annulus, in which the annulus has been replaced by a narrow section missing cortical material, resulting in a hairpin-like structure [ 9 , 27 , 28 ]. The annulus of the spermatozoa has long been thought to have two important putative functions: one is a diffusion barrier function, which is critical for fertilization by retaining proteins in diverse compartments of the sperm tail to the neck [ 29 , 30 ]. The second one could be on the morphological planner function, which directs flagellum growth and mitochondrial connection along the axoneme [ 31 ].…”

Section: Discussionmentioning

confidence: 99%

The correlation of Septin4 gene expression with sperm quality, DNA damage, and oxidative stress level in infertile patients

Jannatifar,

Piroozmanesh,

Jalalabadi

et al. 2023

Anat Cell Biol

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Septin4 belong to a family of polymerizing GTP-binding proteins that are required for many cellular functions, such as membrane compartmentalization, vesicular trafficking, mitosis, and cytoskeletal remodeling. Since, Septin4 is expressed specifically in the testis, we aimed to determine the association between Septin4 gene expression with sperm quality, DNA damage, and stress oxidative level in infertile patients. The present study included 60 semen samples that grouped into three groups: normozoospermia (n=20), asthenozoospermia (n=20), astheno-teratozoospermia (n=20). Initially, semen parameters were analyzed by using the World Health Organization protocol. The mRNA expression of Septin4 in sperm was examined using reverse transcription-polymerase chain reaction. Oxidative stress markers, i.e., total antioxidant capacity, superoxide dismutase, catalase, glutathione peroxidase, and malondialdehyde, were determined by ELISA kit. The current study showed a statistically significant highly positive correlation in Septin4 gene expression with sperm motility, normal morphology, viability, capacity, and sperm mitochondrial membrane potential (MMP). However, it showed significant negative correlation with sperm DNA fragmentation. Septin4 had a significant correlation with stress oxidative factor and antioxidant enzyme levels. In conclusion, Septin4 gene expression provides clinical useful information for the diagnosis of male infertility. It might be a marker for discrimination between fertile and infertile patients. The current study showed a statistically significant highly positive correlation in Septin4 gene expression with sperm motility, normal morphology, viability, capacity, and sperm MMP. However, it shows significant negative correlation with sperm DNA fragmentation. Septin4 had a significant correlation with stress oxidative factor and antioxidant enzyme levels.

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Section: Discussionmentioning

confidence: 99%

The correlation of Septin4 gene expression with sperm quality, DNA damage, and oxidative stress level in infertile patients

Jannatifar,

Piroozmanesh,

Jalalabadi

et al. 2023

Anat Cell Biol

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“…Other researchers worked with Septin-4-null sperm or flagella modified with no annulus, this structure has been replaced by thin segment missing cortical material, acts like an abnormal-flagella conferring a hairpinlike structure (56)(57)(58). Two major hypothetical utilities have long been ascribed to the annulus of the spermatozoa: one is a diffusion barrier function; it is a very essential function for the fertilization, detaining proteins to various compartments of the sperm tail to the neck (59,60). The second one is might be on morphological planner function given guidance to the growth of the flagellum and the association of the mitochondria along the axoneme.…”

Section: Discussionmentioning

confidence: 99%

Identification and Structure Prediction of Human Septin-4 as a Biomarker for Diagnosis of Asthenozoospermic Infertile Patients—Critical Finding Toward Personalized Medicine

et al. 2021

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Semen parameters are been found as a key factor to evaluate the count and morphology in the given semen sample. The deep knowledge of male infertility will unravel with semen parameters correlated with molecular and biochemical parameters. The current research study is to identify the motility associated protein and its structure through the in-silico approach. Semen samples were collected and initial analysis including semen parameters was analyzed by using the World Health Organization protocol. Semen biochemical parameters, namely, seminal plasma protein concentration, fructose content, and glucosidase content were calculated and evaluated for correlation. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) were carried out for identification of Septin-4 presence in the semen sample. Mascot search was done for protein conformation and in-silico characterization of Septin-4 by structural modeling in Iterative Threading Assembly Refinement (I-TASSER). Twenty-five nanoseconds molecular dynamics (MD) simulations results showed the stable nature of Septin-4 in the dynamic system. Overall, our results showed the presence of motility-associated protein in normospermia and control samples and not in the case of asthenospermia and oligoasthenospermia. Molecular techniques characterized the presence of Septin-4 and as a novel biomarker for infertility diagnosis.

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“…This could also affect sperm morphology. It is also concerned with changing the membrane surface of maturing spermatozoa including stabilization of the acrosome and its membrane during sperm maturation and the coating of spermatozoa with certain antigens, which play an important role in the development of fertilizing capacity of spermatozoa (Levinsky et al, 1983; La Spina et al, 2017). According to Samova et al (2016), BPA exposure causes the morphological abnormality in mice spermatozoa.…”

Section: Discussionmentioning

confidence: 99%

In vivo analysis of Bisphenol A induced dose-dependent adverse effects in cauda epididymis of mice

Samova

Doctor

Verma

2018

Interdisciplinary Toxicology

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Bisphenol A is widely used as a material for the production of epoxy resins and polycarbonate plastics. It contaminates various food stuffs by getting leached out from their container lining. Limited information is available on its effects on the male reproductive system. The aim of the present study was to evaluate the extent to which bisphenol A can affect the reproductive system by measuring biochemical and histological changes in the epididymis. Inbred Swiss strain male albino mice were orally administered 80, 120 and 240 mg/kg body weight/day of BPA for 45 days. After completion of treatment, the animals were sacrificed; cauda epididymis was isolated, weighed, used for biochemical and histopathological studies. The results revealed that BPA administered for 45 days caused significant (p<0.05) and dose-dependent reduction in epididymis weight. There was significant (p<0.05) increase in lipid peroxidation and the acid phosphatase activity. Dose dependent reduction in protein, sialic acid contents, as well as the activity of enzymatic antioxidants and mitochondrial enzymes was recorded compared to vehicle treated group. The effect was dose-dependent. Histopathological alteration was observed. This study concludes that BPA causes toxicity in epididymis of mice by generating free radicals, which may be a possible reason for reduction in sperm parameters.

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Molecular and Cellular Aspects of Mammalian Sperm Acrosomal Exocytosis

Cited by 4 publications

References 216 publications

The correlation of Septin4 gene expression with sperm quality, DNA damage, and oxidative stress level in infertile patients

The correlation of Septin4 gene expression with sperm quality, DNA damage, and oxidative stress level in infertile patients

Identification and Structure Prediction of Human Septin-4 as a Biomarker for Diagnosis of Asthenozoospermic Infertile Patients—Critical Finding Toward Personalized Medicine

In vivo analysis of Bisphenol A induced dose-dependent adverse effects in cauda epididymis of mice

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