Molecular and Biomechanical Characterization of Mineralized Tissue by Dental Pulp Cells on Titanium

Nakamura, Hiromi; Saruwatari, Lei; Aita, Hideki; Takeuchi, Kazuo; Ogawa, Takahiro

doi:10.1177/154405910508400606

Cited by 59 publications

(52 citation statements)

References 25 publications

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“…Immediately after the incisors were surgically extracted, the dental pulp was retrieved and minced pulp was digested with phosphate-buffered saline (PBS) containing 0.1% collagenase and 0.25% trypsin. 21 DPSCs were cultured with alpha modification of the Eagle's medium (a-MEM) (GIBCO) supplemented with 20% fetal bovine serum (GIBCO). DPSCs from passage 3 to 6 were used for all experiments.…”

Section: Isolation and Cell Culture Of Dpscsmentioning

confidence: 99%

Mechanical Stretch Increases the Proliferation While Inhibiting the Osteogenic Differentiation in Dental Pulp Stem Cells

Hara

Naruse

Ozawa

et al. 2013

Tissue Engineering Part A

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Dental pulp stem cells (DPSCs), which can differentiate into several types of cells, are subjected to mechanical stress by jaw movement and occlusal forces. In this study, we evaluated how the uniaxial mechanical stretch influences proliferation and differentiation of DPSCs. DPSCs were isolated and cultured from male SpragueDawley rats. Cultured DPSCs were identified by surface markers and the differentiation capabilities as adipocytes or osteoblasts. To examine the response to mechanical stress, uniaxial stretch was exposed to cultured DPSCs. We evaluated the impact of stretch on the intracellular signaling, proliferation, osteogenic differentiation, and gene expressions of DPSCs. Stretch increased the phosphorylation of Akt, ERK1/2, and p38 MAP kinase as well as the proliferation of DPSCs. The stretch-induced proliferation of DPSCs was abolished by the inhibition of the ERK pathway. On the other hand, stretch significantly decreased the osteogenic differentiation of DPSCs, but did not affect the adipogenic differentiation. We also confirmed mRNA expressions of osteocalcin and osteopontin were significantly suppressed by stretch. In conclusion, uniaxial stretch increased the proliferation of DPSCs, while suppressing osteogenic differentiation. These results suggest a crucial role of mechanical stretch in the preservation of DPSCs in dentin. Furthermore, mechanical stretch may be a useful tool for increasing the quantity of DPSCs in vitro for regenerative medicine.

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Section: Isolation and Cell Culture Of Dpscsmentioning

confidence: 99%

Mechanical Stretch Increases the Proliferation While Inhibiting the Osteogenic Differentiation in Dental Pulp Stem Cells

Hara

Naruse

Ozawa

et al. 2013

Tissue Engineering Part A

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“…Cells were then washed twice and re-suspended in complete medium (DMEM supplemented with 10% FBS, 1% antibiotic-antimycotic, 1% non-essential amino acids, 1% Na pyruvate). Dental pulp stromal cells were then cultured with ascorbic acid (50 μg/ml) and Na-β-glycerophosphate (10mM) (Sigma Aldrich, MO) with or without dexamethasone (10 −8 M) [22] to induce different degrees of differentiation as indicated in the result section. The cells were then cultured at 37°C in 5% CO 2 , and they were passaged and used in the experiments at 80% confluency.…”

Section: Dental Pulp Stromal Cell Culturesmentioning

confidence: 99%

N-acetylcysteine protects dental pulp stromal cells from HEMA-induced apoptosis by inducing differentiation of the cells

Paranjpe

Cacalano

Hume

et al. 2007

Free Radical Biology and Medicine

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Resin based materials are now widely used in dental restorations. While the use of these materials is aesthetically appealing in patients, they carry the risk of local and systemic adverse effects. The potential risks are direct damage to the cells and induction of immune-based hypersensitivity reactions. Dental Pulp Stromal Cells (DPSCs) and oral keratinocytes are the major cell types which may come in contact with dental resins such as 2-hydroxyethyl methacrylate (HEMA) after dental restorations. Here we show that N-acetyl cysteine (NAC) inhibits HEMA induced apoptotic cell death and restores the function of DPSCs and oral epithelial cells. NAC inhibits HEMA mediated toxicity through induction of differentiation in DPSCs since the genes for dentin sialoprotein (DSP), Osteopontin (OPN), Osteocalcin (OCN), and Alkaline Phosphatase (ALP) which are induced during differentiation are also induced by NAC. Unlike NAC, Vitamin E and C which known anti-oxidant compounds failed to prevent either HEMA mediated cell death or decrease in VEGF secretion by human DPSCs. More importantly, when added either alone or in combination with HEMA Vitamin E and Vitamin C did not increase the gene expression for OPN, and in addition Vitamin E inhibited the protective effect of NAC on DPSCs. NAC inhibited HEMA mediated decrease in NFκB activity thus, providing survival mechanism for the cells. Overall, the studies reported in this paper indicated that undifferentiated DPSCs have exquisite sensitivity to HEMA induced cell death, and their differentiation by NAC resulted in an increased NFκB activity which might have provided the basis for their increased protection from HEMA mediated functional loss and cell death.

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“…Besides that, DPSCs were effective in regenerating bone tissue, 14,15,16 hair follicles, 17 heart muscle, 18,19 neu rona l cel ls, 20 a nd cor nea l epit hel iu m. 21 Furthermore, these stem cells are immunoprivileged and have the ability to modulate immune and inflammatory responses.…”

Section: Introductionmentioning

confidence: 99%

Culture of human dental pulp cells at variable times post-tooth extraction

Araújo

Oliveira²,

et al. 2018

Braz. oral res.

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The aim of this study was to investigate the viability of human dental pulp cells from extracted teeth kept at standard room temperature and atmospheric pressure for different periods of time. Twenty-one healthy permanent teeth were used. They were divided into five groups according to the expected time from extraction to processing. One group was tested immediately after extraction; the other groups were each tested at one of the following time points: 30 minutes, 1 hour, 2 hours, and 5 hours post-extraction. Cell morphology was analysed by light microscopy; cell proliferation was analysed using MTT assay and by counting the viable cells in a haemocytometer. Similar results were observed in all groups (p < 0.05). A delay of up to five hours for tooth processing and tissue collection does not preclude the establishment of dental pulp cell cultures, affect the morphology of these cells, or reduce their proliferative potential.

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Molecular and Biomechanical Characterization of Mineralized Tissue by Dental Pulp Cells on Titanium

Cited by 59 publications

References 25 publications

Mechanical Stretch Increases the Proliferation While Inhibiting the Osteogenic Differentiation in Dental Pulp Stem Cells

Mechanical Stretch Increases the Proliferation While Inhibiting the Osteogenic Differentiation in Dental Pulp Stem Cells

N-acetylcysteine protects dental pulp stromal cells from HEMA-induced apoptosis by inducing differentiation of the cells

Culture of human dental pulp cells at variable times post-tooth extraction

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