Molecular and biological characterization of a new mulberry idaeovirus

Gürcan, Kahraman; Turan, Sibel; Teber, S.; Kılınçer, İlyas; Uz, İbrahim; Tamisier, Lucie; Massart, Sébastien; Çağlayan, Kadriye

doi:10.1016/j.virusres.2021.198411

Cited by 6 publications

(3 citation statements)

References 26 publications

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“…The genus Ideovirus includes five proposed species: citrus idaeovirus (Derrick et al, 2006), privet leaf blotch-associated virus (Navarro et al, 2017), blackcurrant leaf chlorosis-associated virus (James & Phelan, 2017), green Sichuan pepper-idaeovirus (Cao et al, 2019), and black mulberry idaeovirus (Gürcan et al, 2021). Raspberry bushy dwarf virus (RBDV), a seed and pollen-borne Idaeovirus (Mayoviridae), infects mainly Rubus species.…”

Section: Introductionmentioning

confidence: 99%

Incidence and genetic diversity of raspberry bushy dwarf virus (RBDV) in Rubus spp. in Turkey

Çağlayan

Ördek

Gazel

et al. 2023

Annals of Applied Biology

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Raspberry bushy dwarf virus (RBDV), recently renamed to Idaeovirus rubi, is one of the most common viruses infecting Rubus species worldwide but there is still a limited number of genome sequences available in the GenBank database and the majority of the sequences include partial sequences of RNA‐1 and RNA‐2. The distribution and incidence of RBDV in main raspberry and blackberry growing provinces in Turkey were monitored during 2015–2019 and 537 Rubus spp. samples were tested by both DAS‐ELISA and RT‐PCR. Among the tested samples, 36 samples tested positive for RBDV by DAS‐ELISA and 67 samples by RT‐PCR. There was relatively low nucleotide diversity among the Turkish isolates. Turkish isolates shared 93%–97.7%, 84.3%–98.9%, and 85%–99.2% nucleotide sequence identities with available sequences in the GenBank, in partial RNA‐1, movement protein (MP) and coat protein (CP) genes, respectively. In the phylogenetic tree constructed for RNA‐1, MP, and CP sequences, all Turkish raspberry isolates were clustered in a distinct clade. However, the blackberry isolates showed considerable variation in nucleotide sequences and were placed in three distinct groups. The divergent blackberry isolates showed high variability in MP (84.5%–89.3%) and CP (85.5%–89.7%) regions and were placed in a distinct group. The rest of blackberry isolates clustered together with sweet cherry RBDV isolates adjacent to the grapevine clade or together with raspberry isolates. The comparative analysis conducted on three RNA segments of RBDV highlighted the high sequence diversity of Turkish RBDV isolates. This study also emphasizes the importance of regular monitoring of RBDV infections in Turkey, with special regard to those Rubus spp. and grapevine accessions employed in conservation and selection programmes. In particular, the presence of new RBDV genetic variants and infection of Rubus species must be taken into account to choose a correct detection protocol and management strategy.

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Section: Introductionmentioning

confidence: 99%

Incidence and genetic diversity of raspberry bushy dwarf virus (RBDV) in Rubus spp. in Turkey

Çağlayan

Ördek

Gazel

et al. 2023

Annals of Applied Biology

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“…Mulberry crinkle leaf virus (MCLV) [ 7 ], mulberry mosaic dwarf-associated virus (MMDaV) [ 8 ], mulberry cryptic virus 1 (MuCV1) [ 9 ], and citrus leaf blotch virus-ML (CLBV-ML) [ 10 ] all cause mosaic symptoms in mulberry trees. Mulberry badnavirus 1 (MBV1) [ 11 ] affects both leaf and fruit development. In contrast, there are also some viruses present in mulberry trees that do not cause significant damage due to their low pathogenic ability and low accumulation, such as Citrus leaf blotch virus isolate mulberry alba 2 (CLBV-ML2) and Mulberry-associated virga-like virus (MaVLV) [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Identification and Characterization of a Novel Quanzhou Mulberry Virus from Mulberry (Morus alba)

Jia

Chen

et al. 2023

Viruses

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In this study, we discovered a new virus named Quanzhou mulberry virus (QMV), which was identified from the leaves of an ancient mulberry tree. This tree is over 1300 years old and is located at Fujian Kaiyuan Temple, a renowned cultural heritage site in China. We obtained the complete genome sequence of QMV using RNA sequencing followed by rapid amplification of complementary DNA ends (RACE). The QMV genome is 9256 nucleotides (nt) long and encodes five open reading frames (ORFs). Its virion was made of icosahedral particles. Phylogenetic analysis suggests that it belongs to the unclassified Riboviria. An infectious clone for QMV was generated and agroinfiltrated into Nicotiana benthamiana and mulberry, resulting in no visible disease symptoms. However, systemic movement of the virus was only observed in mulberry seedlings, suggesting that it has a host-specific pattern of movement. Our findings provide a valuable reference for further studies on QMV and related viruses, contributing to the understanding of viral evolution and biodiversity in mulberry.

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“…Mulberry mosaic dwarf-associated virus (MMDaV) caused mosaic and dwarfing symptoms in mulberry trees [13], and mulberry cryptic virus 1 (MuCV1) exhibited yellow vein symptoms [14]. The symptoms caused by mulberry badnavirus 1 (MBV-1) include mosaic structure, deformation, vein clearing and necrosis on the leaves and deformation, crumbling, and scabs on the fruits [15]. Citrus leaf blotch virus-ML (CLBV-ML) caused symptoms of chlorotic leaf spots and occasionally witches' broom [16].…”

Section: Introductionmentioning

confidence: 99%

Identification of Three Viruses Infecting Mulberry Varieties

Chen

Liu

et al. 2022

Viruses

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Viruses-mediated genome editing in plants is a powerful strategy to develop plant cultivars with important and novel agricultural traits. Mulberry alba is an important economic tree species that has been cultivated in China for more than 5000 years. So far, only a few viruses have been identified from mulberry trees, and their application potential is largely unknown. Therefore, mining more virus resources from the mulberry tree can pave the way for the establishment of useful engineering tools. In this study, eight old mulberry plants were gathered in seven geographic areas for virome analysis. Based on transcriptome analysis, we discovered three viruses associated with mulberries: Citrus leaf blotch virus isolate mulberry alba 2 (CLBV-ML2), Mulberry-associated virga-like virus (MaVLV), and Mulberry-associated narna-like virus (MaNLV). The genome of CLBV-ML2 was completely sequenced and exhibited high homology with Citriviruses, considered to be members of the genus Citrivirus, while the genomes of MaVLV and MaNLV were nearly completed lacking the 5′ and 3′ termini sequences. We tentatively consider MaVLV to be members of the family Virgaviridae and MaNLV to be members of the genus Narnavirus based on the results of phylogenetic trees. The infection experiments showed that CLBV-ML2 could be detected in the inoculated seedlings of both N. benthamiana and Morus alba, while MaVLV could only be detected in N. benthamiana. All of the infected seedlings did not show obvious symptoms.

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Molecular and biological characterization of a new mulberry idaeovirus

Cited by 6 publications

References 26 publications

Incidence and genetic diversity of raspberry bushy dwarf virus (RBDV) in Rubus spp. in Turkey

Incidence and genetic diversity of raspberry bushy dwarf virus (RBDV) in Rubus spp. in Turkey

Identification and Characterization of a Novel Quanzhou Mulberry Virus from Mulberry (Morus alba)

Identification of Three Viruses Infecting Mulberry Varieties

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